Abstract:
:The polymerase chain reaction (PCR) allows the detection of minimal amounts of nucleic sequences and has been successfully used to test for the chronic myeloid leukemia-specific bcr/abl transcripts. We studied blood samples from 17 patients who had undergone allogeneic bone marrow transplantation for CML, using a modified polymerase chain reaction-based assay for the detection of leukemic mRNA. This nested PCR technique was found to be highly sensitive, detecting the chimeric bcr/abl transcript in 16 of 17 patients including several long-term survivors. Cytogenetic techniques failed to detect Ph mitoses. The clinical significance of the persisting bcr/abl transcript for long periods following BMT is poorly understood and remains to be elucidated by further studies.
journal_name
Leukemiajournal_title
Leukemiaauthors
Pignon JM,Henni T,Amselem S,Vidaud M,Duquesnoy P,Vernant JP,Kuentz M,Cordonnier C,Rochant H,Goossens Msubject
Has Abstractpub_date
1990-02-01 00:00:00pages
83-6issue
2eissn
0887-6924issn
1476-5551journal_volume
4pub_type
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