Abstract：
OBJECTIVE:In heart failure (HF), the generation of reactive oxygen species (ROS) is enhanced. It was shown that failing cardiac myocytes are more susceptible to ROS-induced damage, possibly due to increased expression of the sarcolemmal Na-Ca exchanger (NCX). METHODS:We investigated the consequences of increased expression levels of NCX in adult rabbit ventricular cardiomyocytes (via adenovirus-mediated gene transfer, Ad-NCX1-GFP) with respect to tolerance towards ROS. After 48-h incubation, cells were monitored for morphological changes on an inverted microscope. ROS were generated via hydrogen peroxide (H(2)O(2)) (100 micromol/l) and Fe(3+)/nitrilotriacetate (Fe(3+)/NTA, 100/200 micromol/l) for 4 min and cell morphology was followed over 30 min. [Na(+)](i) and [Ca(2+)](i) in native cells were measured using SBFI-AM and Indo1-AM, respectively. RESULTS:In native myocytes, exposure to ROS induced hypercontracture. This was accompanied by a 1.3-fold increase in diastolic Indo1 fluorescence ratio (P<0.05). Overexpression of NCX significantly enhanced development of hypercontracture. After 15 min, the percentage of cells that had undergone hypercontracture (F(hyper)) was 85+/-4% vs. only 44+/-10% in control cells (P<0.05). Inhibition of NCX-mediated Ca(2+) entry with KB-R7943 (5 micromol/l) reduced F(hyper) to 33+/-11% (P<0.05). [Na(+)](i) was increased 2.9-fold 1 min prior to hypercontracture (P<0.05). CONCLUSIONS:ROS-induced hypercontracture is due to Ca(2+) entry via NCX which could be triggered by a concomitant substantial increase in [Na(+)](i). Elevated NCX levels predispose to ROS-induced injury, a mechanism likely contributing to myocyte dysfunction and death in heart failure.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Wagner S,Seidler T,Picht E,Maier LS,Kazanski V,Teucher N,Schillinger W,Pieske B,Isenberg G,Hasenfuss G,Kögler Hdoi
10.1016/j.cardiores.2003.08.006subject
Has Abstractpub_date
2003-11-01 00:00:00pages
404-12issue
2eissn
0008-6363issn
1755-3245pii
S0008636303005716journal_volume
60pub_type
杂志文章abstract：AIMS:The discovery of a specific prorenin receptor (PRR) suggests a biological function of prorenin that is independent of angiotensin I production. In the present study, we investigated the role of PRR on smooth muscle cell (SMC) migration. METHODS AND RESULTS:PRR was expressed in human mammary arteries and in cultur...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvs204
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:Anisotropic conduction characteristics, which may be expressed as the ratio of conduction velocities in the longitudinal (Vlong) and transverse (Vtrans) fibre directions, have been shown to stabilise reentry and favour the induction of sustained, uniform ventricular tachycardia. The aim of this study was to i...
journal_title：Cardiovascular research
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doi：10.1016/s0008-6363(96)88593-2
更新日期：1995-03-01 00:00:00
abstract：:Microelectrode recordings of skin nerve sympathetic activity were made in the median nerve supplying the right hand of nine patients with Raynaud's phenomenon and 10 control subjects. With subjects warmed up (finger temperature 33 to 34 degrees C) different manoeuvres were used to evoke strong, single sympathetic burs...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/19.5.249
更新日期：1985-05-01 00:00:00
abstract：AIMS:Hyperhomocysteinaemia is an independent risk factor for atherosclerotic vascular disease and is associated with vascular endothelial dysfunction. Homocysteine modulates cellular methylation reactions. P66shc is a protein that promotes oxidative stress whose expression is governed by promoter methylation. We asked ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvr250
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:Stretch-activated cation channels (SAC) have been suggested to act as endothelial mechanosensors for hemodynamic forces. Ca(2+) influx through SAC could induce an intracellular Ca(2+) signal stimulating Ca(2+)-dependent synthesis of vasodilators like NO, prostacyclin, or EDHF. In the present study we tested w...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(01)00476-x
更新日期：2002-01-01 00:00:00
abstract：AIMS:Peripheral chemoreflex sensitivity is potentiated in both clinical and experimental chronic heart failure (CHF). NADPH oxidase-derived superoxide mediates angiotensin II (Ang II)-enhanced carotid body (CB) chemoreceptor sensitivity in CHF rabbits, and tempol, the superoxide dismutase (SOD) mimetic, inhibits this A...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvn350
更新日期：2009-03-01 00:00:00
abstract：OBJECTIVES:Plasma endothelin (ET) concentrations are increased in heart failure. The aims of the present study were to investigate to what extent cardiac ET mRNA expression is induced in ischemic heart failure and whether there may be compensatory downregulation of myocardial mRNA levels for the ETA and ETB receptor su...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(96)00266-0
更新日期：1997-03-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/28.9.1326
更新日期：1994-09-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2006.11.001
更新日期：2007-02-01 00:00:00
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journal_title：Cardiovascular research
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doi：10.1093/cvr/cvn228
更新日期：2008-12-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/27.9.1555
更新日期：1993-09-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvu209
更新日期：2014-11-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/13.7.370
更新日期：1979-07-01 00:00:00
abstract：OBJECTIVE:To determine the effect of Na(+)-H+ exchange blockade on ischemic rigor contracture and reperfusion-induced hypercontracture. METHODS:Thirty-six pigs were submitted to 55 min of coronary occlusion and 5 h reperfusion. Myocardial segment length analysis with ultrasonic microcrystals was used to detect ischemi...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(97)00106-5
更新日期：1997-07-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/27.7.1359
更新日期：1993-07-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/cvs303
更新日期：2013-03-15 00:00:00
abstract：OBJECTIVE:A previous study from this laboratory showed that elevation of endogenous angiotensin II (Ang II) and upregulation of the angiotensin II type 1 (AT(1)) receptor in the carotid body (CB) are involved in the enhanced peripheral chemoreceptor sensitivity in rabbits with chronic heart failure (CHF). NADPH oxidase...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2007.04.006
更新日期：2007-08-01 00:00:00
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doi：10.1093/cvr/cvz345
更新日期：2021-01-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/20.5.379
更新日期：1986-05-01 00:00:00
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journal_title：Cardiovascular research
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doi：10.1093/cvr/9.4.538
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更新日期：2011-01-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2007.06.016
更新日期：2007-09-01 00:00:00
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journal_title：Cardiovascular research
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doi：10.1016/j.cardiores.2007.02.023
更新日期：2007-05-01 00:00:00
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journal_title：Cardiovascular research
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doi：10.1093/cvr/cvp213
更新日期：2009-11-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvp055
更新日期：2009-07-15 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/s0008-6363(01)00288-7
更新日期：2001-08-15 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/11.6.568
更新日期：1977-11-01 00:00:00
abstract：OBJECTIVE:The aim was to investigate the influence of coronary occlusion on myocardial interstitial noradrenaline levels in the ischaemic and non-ischaemic regions, using the dialysis technique. METHODS:In nine anaesthetised cats, regional ischaemia was produced by occlusion of the left anterior descending coronary ar...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/27.5.817
更新日期：1993-05-01 00:00:00
