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Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle.

Abstract：

:Plasmid pRSVL persisted and expressed luciferase for at least 19 months in mouse skeletal muscle after intramuscular injection. Other injected plasmids also stably expressed long-term suggesting that any plasmid DNA could stably persist and express in muscle. Plasmid DNA was demonstrated by quantitative PCR in some of the muscle DNA samples for at least 19 months after injection. The methylation pattern of the plasmid DNA remained in its bacterial form indicating that the foreign DNA did not replicate in the muscle cells. The electroporation of total cellular DNA from injected muscles into bacteria indicated that the plasmid DNA was extrachromosomal. Chromosomal integration of plasmid DNA was searched for by electroporating the injected muscle DNA into bacteria after restriction enzyme digestion and ligation. No plasmids containing plasmid/chromosome junctions were observed in over 1800 colonies examined. Lack of integration increases the theoretical safety of this gene transfer technique. Long-term stability of plasmid DNA in muscle indicates that muscle is an attractive target tissue for the introduction of extrachromosomal plasmid or viral DNA for the purpose of gene therapy.

journal_name

Hum Mol Genet

journal_title

Human molecular genetics

authors

Wolff JA,Ludtke JJ,Acsadi G,Williams P,Jani A

doi

10.1093/hmg/1.6.363

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

363-9

issue

6

eissn

0964-6906

issn

1460-2083

journal_volume

1

pub_type

杂志文章

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