Abstract：
:Carbocysteine lysine salt monohydrate (SCMC-Lys) is a well-known mucoactive drug whose therapeutic efficacy is commonly related to the ability of SCMC-Lys to replace fucomucins by sialomucins. The aim of this study was to determine if SCMC-Lys could exert an anti-oxidant action by scavenging reactive oxygen intermediates (ROIs). Our results show that SCMC-Lys proved effective as a selective scavenger of hypochlorous acid (HOCl) and hydroxyl radical (OH.), this effect being related to the reactivity of the SCMC tioether group. The scavenger activity of SCMC-Lys was observed in free cellular system as well as in activated human polymorphonuclear neutrophils (PMNs). SCMC-Lys scavenger activity on HOCl was paralleled by a powerful protection from HOCl-mediated inactivation of alpha1-antitripsin (alpha1-AT) inhibitor, the main serum protease inhibitor. Production of interleukin-(IL-)8, a major mediator of PMN recruitment in inflammatory diseases, is known to be mediated by intracellular OH. SCMC-Lys significantly reduced IL-8 production on stimulated human peripheral blood mononuclear cells (PBMCs) in the same range of concentrations affecting OH. activity. It is concluded that SCMC-Lys could exert, in addition to its mucoactive capacity, an anti-oxidant action, thus contributing to the therapeutic efficacy of SCMC-Lys.
journal_name
Eur Cytokine Netwjournal_title
European cytokine networkauthors
Brandolini L,Allegretti M,Berdini V,Cervellera MN,Mascagni P,Rinaldi M,Melillo G,Ghezzi P,Mengozzi M,Bertini Rsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
20-6issue
1eissn
1148-5493issn
1952-4005journal_volume
14pub_type
杂志文章abstract：:Recently there has been great interest in the ex vivo expansion and/or purging of human bone marrow cells prior to transplantation in order to obtain a long-lasting restoration of normal hematopoiesis and freedom from relapse. Long-term human bone marrow cultures (LTHBMC) represent the best available approximation of ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Dendritic cells (DCs) are professional, antigen-presenting cells, which induce and regulate T cell reactivity. DCs are crucial in innate and adaptive immune responses, and are also involved in central and peripheral tolerance induction. Tolerance can be mediated by immature and semi-mature DCs expressing low levels of...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2007.0089
更新日期：2007-06-01 00:00:00
abstract：:Leptin may support the proliferation and hinder the apoptosis of tumor cells. Although leptin expression has been studied in several human tumors, its potential clinical significance remains uncertain in patients with gastric carcinoma. Furthermore, the majority of available findings have been determined from preclini...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2018.0408
更新日期：2018-06-01 00:00:00
abstract：:Dendritic cells (DC) can be obtained from peripheral blood mononuclear cells (PBMC) by in vitro culture with IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-13 shares properties with IL-4 but its receptor does not involve the common gamma chain present in the receptor complex of IL-4 and other c...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:No biological parameter is currently available as a specific marker of multiple sclerosis (MS) activity. The aim of this study was to determine whether an evolution of the neurological disability is associated with a modified profile of cytokine production. Clinical disease activity was quantitated by the Kurtzke's ex...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：:We have previously shown that intrathymic (i.t.) injection of staphylococcal enterotoxin B (SEB) to mice induces both T cell clonal deletion and IL-2-dependent anergy. In the present study, we have used a quantitative RT-PCR to demonstrate that i.t. administration of SEB induced a significant decrease in the levels of...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:Fetal bovine sera (FBS) from several commercial suppliers were fractionated by gel filtration. Interleukin-1 (IL-1) activity was bioassayed using the IL-1-specific murine T cell line D10(N4)M. All the sera examined contained IL-1-like activity, with molecular weights (M(r)) of 30 kDa and 15-10 kDa. Under isoelectric f...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:In a recent phase I study of inhalative, human natural interleukin-2 (hnIL-2) treatment of pulmonary metastases from previously resected solid tumors (mainly renal carcinoma), we have reported that this treatment resulted in an increased accessory function of alveolar macrophages (AM) [1]. Encouraged by these data, we...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：:Erythroid nuclear cells have been shown to exert regulatory effects on immunopoiesis. We have reported that some of these influences might be mediated via soluble factors secreted by nuclear erythroid cells. In this report we describe our estimate of the cytokine gene expression in cells isolated from individual eryth...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Autoimmune hepatitis (AIH) is a chronic hepatitis of unknown etiology and several cytokines have been implicated in its pathogenesis and onset. Our objective was to determine the profile of pro and anti-inflammatory cytokines, including IL-1β, IL-6, IL-8, IL-23, IFN-, TNF-α, IL-10 in autoimmune hepatitis and their ass...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2020.0450
更新日期：2020-12-03 00:00:00
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journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:Suggested mechanisms for the systemic, circulating cytokinemia observed during heavy physical exertion include inflammation and energy demand. We compared cytokine levels and examined the underlying physiological mechanisms between a long-distance triathlon and a 100-km run, two endurance races of similar duration but...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:We have previously reported that breast cancer patients and some healthy subjects show positive T-cell-mediated immune responses to a semi-purified mouse mammary tumour viral pool (MMTV). We have now used Western blotting to analyse the specificity of the response and to determine the target polypeptides. Two types of...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Interaction between the CD40 ligand and its cognate receptor is known to affect various aspects of B-cell biology. Less is known about the biological consequences of B-cell signaling through tumor necrosis factor alpha (TNF-alpha) and its two receptors. We have used Ramos germinal center (GC)-derived Burkitt's lymphom...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:IL-1beta is suspected to be involved in the diarrhea that always accompanies inflammatory bowel disease. This work was aimed at studying the in vivo effect of IL-1beta on the net absorption of fluid, Na(+) and Cl(-) from the rat colon, and at delineating its mechanism of action. Rats were injected i.p. with IL-1beta (...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：:Interleukin-1 (IL-1) has been implicated in the pathogenesis of rheumatoid arthritis (RA). IL-1alpha gene polymorphism was analysed for the exon V and promoter region in 51 patients with destructive and 47 with non-destructive RA, as well as in 94 controls. The two biallelic polymorphisms in the promoter region and th...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Adoptive immunotherapy with immune effector cells has proved to be potent for treatment of tumors, however neither the attendant criteria for potential clinical efficacy of the injected cells, nor the method to prepare these cells are presently well established. Our procedure of collecting lymphocytes from biological ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:Atopic dermatitis (AD) is an inflammatory disorder of the skin characterized by an impaired immune response. Several effector T cell subsets, such as pro-inflammatory cells like Th9, Th17 and Th22 cells, expressing high levels of IL-9, IL-17 and IL-22, together with the anti-inflammatory, immuno-modulating Treg cells ...
journal_title：European cytokine network
pub_type： 杂志文章,评审
doi：10.1684/ecn.2013.0333
更新日期：2013-03-01 00:00:00
abstract：:We investigated the serum concentration of the tumour necrosis factor (TNF) family ligands (TNF-alpha and TNF-beta) and their soluble receptors (sTNF-R p55 and sTNF-R p75) in 66 patients with rheumatoid arthritis (RA) and 14 healthy subjects as a control group, using an enzyme-linked immunosorbent assay (ELISA). We ex...
journal_title：European cytokine network
pub_type： 临床试验,杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:The clinical evaluation and management of impacted third molars remain challenging. The aim of the present study was to investigate possible associations between follicular tissue cytokines and radiographic manifestations of impacted third molar. The population included 72 patients who underwent surgical extraction of...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2011.0286
更新日期：2011-06-01 00:00:00
abstract：:We have investigated the value of a gene therapy approach for neuroblastoma (NB), based on retroviral transduction of the IL-1beta or TNF-alpha cytokine genes into human NB lines. Secretion of the corresponding cytokine, was demonstrated in all lines, although with considerable quantitative variations. Cytokine gene e...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:TNF-alpha and TNF-beta are both involved in inflammation which regulates homeostasis in adults. We examined the mRNA expression of TNF-alpha and TNF-beta in murine embryonal carcinoma (EC) cell lines (PCC3, PCC4, ECA2 and F9) and trophoblast cell line (PL/B6) using a combined system of reverse transcription and polyme...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
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journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:The immune effector response to Plasmodium falciparum infection involves a finely-tuned interplay between different cell types and cytokines. However, the processes by which they mediate the development of clinical immunity, in areas of different endemicity, are poorly understood. We analyzed circulating levels of pro...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2010.0208
更新日期：2010-12-01 00:00:00
abstract：:A murine pulmonary infection model using Mycobacterium bovis-BCG was used to study the development of Th1 and Th2 type responses in mice lacking a functional IFN-gamma receptor (IFN-gamma R-/-). Strikingly, the IFN-gamma R-/- mice maintained the Th1 response and developed a profound M. bovis-BCG, specific Th2 type imm...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:Interleukin-6 (IL-6) is a pleiotropic cytokine which regulates the immune response, the acute-phase response, hematopoiesis and body energy balance. Genetic polymorphism at -174 position of IL-6 promoter has been recently reported to be linked with insulin resistance, however, with conflicting results. The C allele at...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2004-04-01 00:00:00
abstract：:Anticancer immunotherapy with cytokines is often limited by the occurrence of severe toxicity, particularly in older age groups, which are characterized by a reduced tolerance to antineoplastic therapies. We, and others, have recently demonstrated the efficacy of pulsing procedures with IL-2 as a new therapeutic strat...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Recent studies have shown that the T cell-derived cytokine, interleukin-17 (IL-17), stimulates hematopoiesis, specifically granulopoiesis inducing expansion of committed and immature progenitors in bone marrow. Our previous results pointed to its role in erythropoiesis too, demonstrating significant stimulation of BFU...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
abstract：OBJECTIVE:The effects of statins (3-hydroxy-3-methylglutaryl coenzyme A reductase-HMGR-inhibitors) on the inflammatory response remain unclear. HMGR is implicated in the mevalonate pathway, directly upstream of cholesterol biosynthesis. We studied the impairment by this pathway of cytokine production by peripheral bloo...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2009.0162
更新日期：2009-09-01 00:00:00
abstract：:RANK, RANKL, and OPG have well established regulatory effects on bone metabolism. RANK is expressed at very high levels on osteoclastic precursors and on mature osteoclasts, and is required for differentiation and activation of the osteoclast. The ligand, RANKL binds to its receptor RANK to induce bone resorption. RAN...
journal_title：European cytokine network
pub_type： 杂志文章,评审
doi：
更新日期：2002-04-01 00:00:00
