Abstract：
:We have investigated the value of a gene therapy approach for neuroblastoma (NB), based on retroviral transduction of the IL-1beta or TNF-alpha cytokine genes into human NB lines. Secretion of the corresponding cytokine, was demonstrated in all lines, although with considerable quantitative variations. Cytokine gene expression significantly reduced the proliferation index (p = 0.0001); this effect was associated with either terminal neuronal (one TNF-alpha line) or fibroblast-like differentiation (two IL-1beta lines), leading to growth arrest after a few weeks. Cell surface levels of CD54 and HLA class II remained unaffected, but HLA class I (p < 0.001) and CD58 expression (p = 0.01) increased on SKNSH after TNF-alpha gene transfer. Mononuclear cells from normal allogeneic donors cocultured with both IL-1beta (p < 0.001) and TNF-alpha lines (p < 0.01), showed a significant increase in the proportion of activated T cells (CD3+DR+); however, their cytotoxicity and proliferation rate remained unchanged. Immunotherapy of neuroblastoma will require identification of transduced lines in which cytokine secretion induces phenotypic changes in such a way as to augment their likely immunomodulatory properties without impeding cell growth. Because of the limited efficacy of IL-1beta or TNF-alpha gene transfer alone, further studies should focus on combination with other immunomodulatory agents, to improve their potential efficacy in neuroblastoma.
journal_name
Eur Cytokine Netwjournal_title
European cytokine networkauthors
Coze C,Leimig T,Jimeno MT,Mannoni Psubject
Has Abstractpub_date
2001-03-01 00:00:00pages
78-86issue
1eissn
1148-5493issn
1952-4005journal_volume
12pub_type
杂志文章abstract：:Leishmania parasites persist in their vertebrate host after the treatment-induced clinical cure and in the asymptomatic infection. They confer resistance to reinfection but represent a risk of occurrence of acute leishmaniosis in immunosuppressed conditions. We examined the effects of prolonged dexamethasone administr...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:In this study we examined the cellular origin and the expression levels of interleukin-18 (IL-18), IL-18 receptor (IL-18R) and IL-1beta-converting enzyme (ICE), which activates pro-IL-18, during normal maturation of murine testis. The levels of IL-18, IL-18R and ICE were significantly higher in testicular tissues and ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2008.0116
更新日期：2008-03-01 00:00:00
abstract：:Thrombopoietin (TPO), the primary physiological regulator of platelet production, was initially thought to be a lineage-specific factor acting predominantly on megakaryocytopoiesis. Detailed studies establish that this cytokine mediates biological effects on a broad spectrum of hematopoietic progenitor cells, includin...
journal_title：European cytokine network
pub_type： 杂志文章,评审
doi：
更新日期：1998-09-01 00:00:00
abstract：:Dendritic cells (DC) can be obtained from peripheral blood mononuclear cells (PBMC) by in vitro culture with IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-13 shares properties with IL-4 but its receptor does not involve the common gamma chain present in the receptor complex of IL-4 and other c...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:A panel of monoclonal antibodies against the soluble IL-6 receptor was used to search for linear epitopes by a Pepscan analysis. Two such epitopes were found and the corresponding peptides were synthesized chemically. The peptides were active to inhibit the IL-6 dependent growth of human multiple myeloma cell line and...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:The effects of IL-3, GM-CSF and IL-5 on the expression of CD23 (Fc epsilon RII), CD25 (IL-2R/p55) and CD4 on an eosinophilic cell line (EoL-3) were investigated by flow cytometry. A separate incubation with IL-3, GM-CSF or IL-5 alone, did not induce the expression of CD23, CD25, or CD4. However, a sequential incubatio...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Cells sensitive to the cytocidal effect of tumor necrosis factor (TNF) were protected against this effect when growth in the presence of elevated concentrations of tryptophan. Several other indole derivatives also provided protection against TNF cytotoxicity. Most effective were indole itself and its monomethyl deriva...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：AIM:The aim of this study was to investigate the relationship between four circulating adipocytokines (apelin, vaspin, visfatin, adiponectin) and markers of insulin sensitivity, in the context of polycystic ovary syndrome (PCOS) in adolescents. SUBJECTS AND METHODS:48, obese, adolescent girls (mean age: 15.6±3.4 years...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2011.0279
更新日期：2011-03-01 00:00:00
abstract：:Members of the Toll-like receptor family are essential components of the innate immune system. Herein we report the molecular cloning and characterization of three novel human Toll-like receptors (hTLRs) designated hTLR7, hTLR8, and hTLR9. Human TLR7-9, like the previously described members hTLR1-6 contain an ectodoma...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:The nucleotide sequences containing the rat interleukin-12 p40 gene was determined. Sequencing revealed the presence of six exons and five introns. Analysis of the 5' non-coding region showed the presence of several possible sites involved in cytokine gene regulation at the transcriptional level. Comparison of the ded...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Incubation of murine C2C12 myotubes with tumour necrosis factor-alpha (TNF-alpha) leads to significant changes in protein content and turnover, suggesting that the cytokine exerts direct effects in skeletal muscle. The effects of the cytokine on protein content show a clear bimodal behaviour. At low concentrations (1 ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:The purpose of this study was to evaluate T-cell immunity markers using serial post-transplantation monitoring of cytokine-producing cells during the first post-transplant months for the prediction of acute rejection and potentially chronic rejection of kidney allograft. We followed 57 kidney allograft recipients for ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2017.0397
更新日期：2017-09-01 00:00:00
abstract：:Interleukin-6 (IL-6) is a pleiotropic cytokine which regulates the immune response, the acute-phase response, hematopoiesis and body energy balance. Genetic polymorphism at -174 position of IL-6 promoter has been recently reported to be linked with insulin resistance, however, with conflicting results. The C allele at...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2004-04-01 00:00:00
abstract：:Toll-like receptor (TLR)-4 is an important pattern recognition receptor for Candida albicans, playing a role in innate host defense. We investigated whether there is an association between the TLR4 Asp299Gly or TLR4 Thr399Ile polymorphism, and the occurrence of Candida bloodstream infection. We performed a case-contro...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2006-03-01 00:00:00
abstract：:Using the murine model of type II collagen-induced arthritis (CIA), we studied its evolution over time by histopathological, immunohistochemical and clinical evaluations. The first clinical symptoms appeared 28 days post-inoculation (dpi), with bovine type II collagen, with an average arthritic index of 1.00 +/- 0.48 ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2007.0099
更新日期：2007-09-01 00:00:00
abstract：:The prevalence of obesity has recently increased dramatically and has contributed to the increasing prevalence of various pathological conditions, including type 2 diabetes mellitus, nonalcoholic fatty liver disease, asthma, various types of cancer, cardiovascular and neurodegenerative diseases, and others. Accumulati...
journal_title：European cytokine network
pub_type： 杂志文章,评审
doi：10.1684/ecn.2018.0415
更新日期：2018-09-01 00:00:00
abstract：:Patients with hypogammaglobulinemia have recurrent infections and fail to produce protective antibodies. In order for B cells to mature into antibody producing cells, several other cell types such as macrophages and helper T lymphocytes must be involved. They secrete several mediators such as interleukin-1 (IL-1), IL-...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:IL-1beta is suspected to be involved in the diarrhea that always accompanies inflammatory bowel disease. This work was aimed at studying the in vivo effect of IL-1beta on the net absorption of fluid, Na(+) and Cl(-) from the rat colon, and at delineating its mechanism of action. Rats were injected i.p. with IL-1beta (...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：:In order to examine the specificity of cytokine production during coronary artery bypass graft (CABG) surgery, we serially measured serum levels of monocyte chemoattractant protein-1 (MCP-1), interleukin-8 (IL-8), interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta) and tumor necrosis factor (TNF), in twenty patients ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:TNF-alpha and TNF-beta are both involved in inflammation which regulates homeostasis in adults. We examined the mRNA expression of TNF-alpha and TNF-beta in murine embryonal carcinoma (EC) cell lines (PCC3, PCC4, ECA2 and F9) and trophoblast cell line (PL/B6) using a combined system of reverse transcription and polyme...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:The clinical evaluation and management of impacted third molars remain challenging. The aim of the present study was to investigate possible associations between follicular tissue cytokines and radiographic manifestations of impacted third molar. The population included 72 patients who underwent surgical extraction of...
journal_title：European cytokine network
pub_type： 杂志文章
doi：10.1684/ecn.2011.0286
更新日期：2011-06-01 00:00:00
abstract：:Recently there has been great interest in the ex vivo expansion and/or purging of human bone marrow cells prior to transplantation in order to obtain a long-lasting restoration of normal hematopoiesis and freedom from relapse. Long-term human bone marrow cultures (LTHBMC) represent the best available approximation of ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Oncostatin M (OSM) is known to inhibit the growth of melanocytes and early-stage melanomas, but this ability is lost with melanoma progression. The biological effects of OSM involve the activation of Janus kinases (Jak) and signal transducer and activator of transcription (STAT) factors. Since SOCS (suppressor of cyto...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:A major role of TNF in pulmonary fibrosis is supported by the following evidences obtained from pulmonary fibrosis induced in mice by bleomycin or silica particles; 1) these diseases are associated with a marked and lasting increase of the TNF mRNA within the lung, 2) they can be prevented by a treatment with anti TNF...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Adoptive immunotherapy with immune effector cells has proved to be potent for treatment of tumors, however neither the attendant criteria for potential clinical efficacy of the injected cells, nor the method to prepare these cells are presently well established. Our procedure of collecting lymphocytes from biological ...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:We have previously reported that breast cancer patients and some healthy subjects show positive T-cell-mediated immune responses to a semi-purified mouse mammary tumour viral pool (MMTV). We have now used Western blotting to analyse the specificity of the response and to determine the target polypeptides. Two types of...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Multiple studies have shown that the cytokine leukemia inhibitory factor (LIF) is protective of the myocardium in the acute stress of ischemia-reperfusion. All three major intracellular signaling pathways that are activated by LIF in cardiac myocytes have been linked to actions that protect against oxidative stress an...
journal_title：European cytokine network
pub_type： 杂志文章,评审
doi：10.1684/ecn.2013.0335
更新日期：2013-03-01 00:00:00
abstract：:Interleukin-6 (IL-6) is used as a growth factor by various tumor cells. It binds to a gp80 specific receptor (IL-6R) and then to a gp130 transducing chain. Both receptor chains are released as soluble functional proteins which circulate in biological fluids. With a view to studying the physiological role of these solu...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is a systemic, autoimmune disorder. Monocyte chemoattractant protein 1 (MCP-1), a chemokine involved in the recruitment and migration of monocytes/macrophages, has been shown to be increased in the plasma of SLE patients. The aim of our study was to evaluate the possible association ...
journal_title：European cytokine network
pub_type： 杂志文章,多中心研究
doi：10.1684/ecn.2012.0297
更新日期：2012-03-01 00:00:00
abstract：:Tumor growth and invasion are not only the result of malignant transformation but are also dependent on environmental influences from surrounding stroma, extracellular matrix (ECM), local cytokines and systemic hormones. We have investigated the influence of ECM components on three human breast cancer cell lines of di...
journal_title：European cytokine network
pub_type： 杂志文章
doi：
更新日期：2002-04-01 00:00:00
