Abstract:
:Long chain fatty acids (LCFAs) appear to be powerful stimulants for small bowel adaptation in patients with short bowel syndrome (SBS). However, the dietary lipid content may alter intestinal lipid transport. The aim of this study was to investigate the effects of a high fat diet (HFD) on in vivo lipid absorption and molecular and cellular mechanisms of LCFAs uptake by the remaining bowel. Male Sprague-Dawley rats (240-280) were randomly assigned to one of three groups: sham rats fed normal chow (sham-NC), SBS rats fed NC (SBS-NC) and SBS rats fed HFD (SBS-HFD). SBS rats underwent a 75% small bowel resection. Rats were sacrificed on day 3 or 14. Body weight, fat intake and fat clearance (total fecal fat) were measured twice a week. Fat absorbability was calculated as intake minus clearance and was expressed as percent of intake. Total RNA from the mucosa of duodenum, jejunum and ileum was extracted using TRIZOL Reagent. Northern blot analysis was performed to determine FAT/CD36 mRNA levels. Enterocyte LCFA transport was measured on day 14. LCFA uptake was determined by measuring cellular [3H]-oleate uptake over time (4-120 s). Mean (+/-SE) FAT/CD36 mRNA levels and oleate uptake kinetic parameters were analyzed using ANOVA. Fat absorbability diminished after bowel resection, suggesting fat malabsorption. Remaining bowel in SBS-NC rats responded by an increase in FAT/CD36 mRNA levels in the duodenum and ileum on day 3, and the duodenum and jejunum on day 14 compared to sham-NC animals, and was accompanied by an increase in enterocyte LCFA transport in all segments. Exposure to a HFD for 14 days resulted in significantly increased fat absorbability after 3 days compared to SBS-NC rats. However, FAT/CD36 mRNA levels (vs. SBS-NC) decreased in all segments on day 3. On day 14, FAT/CD36 mRNA levels were decreased in the duodenum and ileum and were accompanied by reduced oleate uptake by isolated enterocytes in the ileum (vs. SBS-NC). In a rat model of SBS, early high fat diet increased lipid absorptive capacity of the intestinal remnant as seen by increased fat absorbability. The main mechanisms of this effect may be an acceleration of structural intestinal adaptation resulting in an increased number of enterocytes. However, at molecular and cellular levels HFD decreased mucosal FAT/CD36 mRNA levels and oleic acid uptake by isolated enterocytes.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Sukhotnik I,Gork AS,Chen M,Drongowski RA,Coran AG,Harmon CMdoi
10.1007/s00383-003-1016-3subject
Has Abstractpub_date
2003-07-01 00:00:00pages
385-90issue
5eissn
0179-0358issn
1437-9813journal_volume
19pub_type
杂志文章abstract::This study describes a subset of patients with posterior urethral valves (PUV) who presented late in childhood. The objective was to identify factors that lead to back-pressure effects on the upper tracts, which persist in spite of adequate valve ablation in some patients, and seek factors that may preserve the upper ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050384
更新日期:1998-09-01 00:00:00
abstract:PURPOSE:Congenital hyperinsulinism is a rare disease, and the newly developed 18 fluoro-L-dihydroxyphenylalanine-positron emission tomography (18F-DOPA PET) examination can detect hyperplastic lesions. Our purpose was to report the results of a nationwide survey on surgical treatment of congenital hyperinsulinism in Ja...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4315-4
更新日期:2018-10-01 00:00:00
abstract::Complete transposition of the penis and scrotum, or prepenile scrotum, is an uncommon congenital malformation. Concomitant genitourinary abnormalities, often life-threatening in nature, are frequently seen, and major malformations involving other organ systems may also be present. We report a newborn in whom complete ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050650
更新日期:1999-01-01 00:00:00
abstract::It is widely accepted that there are degenerative changes and decreased spermatogenesis in the contralateral descended testis (CDT) in unilateral undescended testis (UUDT). While some investigators have postulated that the mechanism may be related to primary (congenital) or secondary (autoimmune, vascular, and neural)...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000532
更新日期:2001-07-01 00:00:00
abstract:PURPOSE:The goal of this study was to determine the degree of consensus in the management of spinal cord tethering (TC) in patients with anorectal malformation (ARM) in a large cohort of European pediatric centers. METHODS:A survey was sent to pediatric surgeons (one per center) members of the ARM-Net Consortium. RES...
journal_title:Pediatric surgery international
pub_type: 杂志文章,多中心研究
doi:10.1007/s00383-017-4105-4
更新日期:2017-08-01 00:00:00
abstract:PURPOSE:Megacystis microcolon intestinal hypoperistalsis syndrome (MMIHS) is a rare and the most severe form of functional intestinal obstruction in the newborn. This congenital condition is associated with non-obstructed urinary bladder, microcolon and decreased or absent intestinal peristalsis. This study was designe...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-011-2954-9
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:This study reviews the results of our previously described modification of the minimally invasive (Nuss) procedure for correction of pectus excavatum. It utilizes a subxyphoid incision with central fixation to maximize safe bar passage and minimize bar displacement. METHODS:Consecutive patients corrected wi...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-017-4195-z
更新日期:2018-01-01 00:00:00
abstract::Recurrent or chronic abdominal pain can be a challenging problem when conventional diagnostic studies fail to identify the cause. It is estimated that up to one-third of children suffer from abdominal pain, and in this population recurrent pain can be even more challenging. Although recurrent right lower quadrant (RLQ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1632-1
更新日期:2006-03-01 00:00:00
abstract:PURPOSE:This study aimed to evaluate the mid-term outcomes of single-stage modified anterior sagittal anorectoplasty (ASARP) for anorectal malformation with vestibular fistula. METHOD:Twenty-six patients with congenital imperforate anus and vestibular fistula underwent single-stage modified sphincter-saving ASARP betw...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3749-1
更新日期:2015-09-01 00:00:00
abstract::Interposing vascularized flaps between urethra and skin sutures is recommended in hypospadias surgery to decrease the rate of urethrocutaneous fistula. For this purpose nine patients with failed results after at least one hypospadias surgery and with insufficient foreskin were included in the study. Tubularized incise...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-0989-2
更新日期:2003-11-01 00:00:00
abstract::Our case report details the clinical course and management of a neonate with acute life-threatening respiratory distress presenting soon after birth. She needed intubation and ventilation. A pericardial cyst was diagnosed after a CT scan and 2D echo investigation. As a temporizing procedure the fluid was tapped and th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-0970-0
更新日期:2003-11-01 00:00:00
abstract::Thyroglossal duct cyst (TGDC) disease, one of the most common developmental neck lesion in the pediatric population, often presents as infected neck mass. The authors reviewed their experience in the management of inflamed TGDC cases, in order to suggest the most efficient approach of these patients regarding the idea...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1562-y
更新日期:2005-12-01 00:00:00
abstract::Adrenal cysts (AC) are rare, particularly in the newborn period. Almost all ACs in newborns were diagnosed on exploratory laparotomy or at autopsy. We report a newborn with an AC that was diagnosed after an exploratory laparotomy due to its rare presentation. This case shows that ACs should be considered in the differ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0759-6
更新日期:2003-06-01 00:00:00
abstract::Congenital pouch colon, a variant of anorectal malformation, is a rare anomaly with a high incidence in North India and although the anatomy and diagnostic features have been well described, the surgical treatment continues to be challenging. This report describes the complications following a less preferred but often...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1623-2
更新日期:2006-05-01 00:00:00
abstract::When presentation is delayed, intussusceptions may be difficult to reduce using standard enema regimens. Our endeavour to minimize the need for surgery in an environment where failed reductions are common has led to the development of an aggressive, non-operative method of reducing intussusceptions. One hundred and si...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-1099-x
更新日期:2004-03-01 00:00:00
abstract:INTRODUCTION:Recent advances in laparoscopic surgery as well as increasing experience with these techniques have led to the selection of laparoscopic surgery for hemi/partial nephroureterectomy in children with a non/poorly functioning moiety in a duplex kidney. There is very little data on the long term follow-up of s...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2883-7
更新日期:2011-07-01 00:00:00
abstract::Congenital pyloric atresia (CPA) is a very rare condition that was first described by Calder in 1749. Commonly, CPA occurs as an isolated lesion, which has an excellent prognosis, but it can also be seen in association with other malformations, which can have a negative impact on the final outcome. The medical records...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-1903-0
更新日期:2007-06-01 00:00:00
abstract:INTRODUCTION:This review aims to (1) assess the breadth of pediatric orthopaedic research in low- and middle-income countries (LMICs) and (2) determine the impact of academic collaboration (an LMIC and a non-LMIC investigator) in published LMIC research. METHODS:Pediatric orthopaedic clinical studies conducted in LMIC...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-018-4412-4
更新日期:2019-03-01 00:00:00
abstract:PURPOSE:The purpose of this study was to evaluate the applicability of the National Institute for Health and Clinical Excellence (NICE) guidelines for imaging studies in children under the age of three with first urinary tract infection (UTI). METHODS:In our cohort of 112 patients, we gathered data regarding the occur...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3257-5
更新日期:2013-03-01 00:00:00
abstract::A 7-year-old female with a type I choledochal cyst underwent an exploratory laparotomy. A Roux-en-Y choledochojejunostomy was done following cyst excision. The patient was re-explored 8 days later for an anastomotic bile leak and high fever. A round-worm was found obstructing the jejunal lumen at the porta hepatis, re...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050394
更新日期:1998-09-01 00:00:00
abstract::An unrecognised right aortic arch (RAA) found at thoracotomy may complicate the repair of oesophageal atresia (OA) and tracheo-oesophageal fistula (TOF). This paper analyses the patient characteristics, peri-operative management, and outcome of 16 infants with a RAA, and proposes management guidelines. Between 1948 an...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050505
更新日期:1999-01-01 00:00:00
abstract::Umbilical hernias occur frequently in children but complications are very rare and thus surgery is not routinely indicated. In this literature review, we report 19 cases of spontaneous evisceration of abdominal contents through umbilical hernias. Precipitating causes included umbilical ulceration or sepsis, crying, re...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-012-3076-8
更新日期:2012-05-01 00:00:00
abstract::Necrotizing fasciitis (NF) is a potentially life-threatening infection of soft tissues. It is characterized by rapid spread of inflammation and infection with widespread necrosis of fascia, subcutaneous tissues, and overlying skin. NF is usually reported in adults with preexisting medical conditions or compromised imm...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1481-y
更新日期:2005-08-01 00:00:00
abstract::This review gives an overview of the disease spectrum of congenital diaphragmatic hernia (CDH). Etiological factors, prenatal predictors of survival, new treatment strategies and long-term morbidity are described. Early recognition of problems and improvement of treatment strategies in CDH patients may increase surviv...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-009-2425-8
更新日期:2009-09-01 00:00:00
abstract::Liver transplantation (LT) for malignancy has had disappointing long-term results due to tumour recurrence. Ex-vivo dissection and auto-transplantation have had poor results when the tumor was obstructing bile ducts. Advances in liver surgery have made extensive liver resection safer, but cases of "unresectable" tumou...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000531
更新日期:2001-07-01 00:00:00
abstract:PURPOSE:Sacrococcygeal teratoma (SCT) is the most common congenital neoplasm in neonates. We wished to assess the long-term functional outcome of children undergoing SCT resection. METHODS:Records of neonates diagnosed with SCT from two surgeons' practices, and operated on between 1970 and 2006, were retrospectively r...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2322-1
更新日期:2009-03-01 00:00:00
abstract:PURPOSE:The Nuss procedure is a minimally invasive procedure for the correction of pectus excavatum. It involves insertion of a substernal metal bar. A feared complication of any implantation procedure is infection, which often requires removal of the implanted device. This report describes the authors' experience with...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3145-z
更新日期:2012-09-01 00:00:00
abstract::A case of retrocecal appendicitis is described in a 6-year-old male child who presented with thigh abscess. The presence of a positive psoas stretch test, feculent discharge, an enteric growth on bacteriological examination, and intraabdominal fluid collection on abdominal ultrasound provided clues to the presence of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1356-7
更新日期:2005-04-01 00:00:00
abstract:PURPOSE:To compare open repair (OR) with thoracoscopic repair (TR) for congenital diaphragmatic hernia (CDH) in neonates. METHODS:Twenty-four neonatal CDH cases diagnosed prenatally or within 6 h of birth at our institute from 2002 to 2012 with mild pulmonary hypertension managed without inhaled nitric oxide, were stu...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3382-9
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND:Variation exists in the diagnostic testing for gastroesophageal reflux (GER) in infants and in the application of surgical therapy. There has been an increase in antireflux surgery (ARS) since the development of laparoscopy but the outcomes in high-risk infants is unclear. This study examines the results of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4281-x
更新日期:2018-07-01 00:00:00