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Analysis of deoxyribonucleic acid replication in human X chromosomes by fluorescence microscopy.

Abstract：

:The genetically inactive, late-replicating human female X chromosome can be effectively distinguished from its more active, earlier-replicating homologue, when cells are grown according to the appropriate BrdU-33258 Hoechst protocol. Results obtained from a fluorescence analysis of DNA replication in X chromosomes are consistent with those from previous autoradiographic studies, but reflect additional sensitivity and resolution offered by the BrdU-Hoechst methodology. Both qualitative and quantitative differences in 33258 Hoechst fluorescence intensity, reflecting alterations in replication kinetics, can be detected between the two X chromosomes in female cells. The pattern of replication in the single X chromosome in male cells is indistinguishable from that of the early female X. Intercellular fluctuations in the distribution of regions replicating early or late in S phase, particularly with reference to the late female X, can be localized to structural bands, suggesting multifocal control of DNA synthesis in X chromosomes.

journal_name

Am J Hum Genet

journal_title

American journal of human genetics

authors

Willard HF,Latt SA

subject

Has Abstract

pub_date

1976-05-01 00:00:00

pages

213-27

issue

3

eissn

0002-9297

issn

1537-6605

journal_volume

28

pub_type

杂志文章

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