Abstract:
:In-gel competitive reassociation (IGCR) is a method of differential subtraction to enrich polymorphic DNA restriction fragments between two DNA samples without probes or specific sequence information. Here, we show that by combining IGCR and expressed sequence tags (EST) array hybridization, polymorphic DNA fragments associated with genes in complex higher organisms (Arabidopsis thaliana) can be effectively screened, demonstrating that this procedure offers a simple and efficient way to obtain gene-associated polymorphic DNA markers.
journal_name
Genome Resjournal_title
Genome researchauthors
Gotoh K,Oishi Mdoi
10.1101/gr.434103subject
Has Abstractpub_date
2003-03-01 00:00:00pages
492-5issue
3eissn
1088-9051issn
1549-5469journal_volume
13pub_type
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