Abstract:
:We have developed a computer program that aligns spliced sequences to genomic sequences, using local alignment algorithms and heuristics to put together a global spliced alignment. Spidey can produce reliable alignments quickly, even when confronted with noise from alternative splicing, polymorphisms, sequencing errors, or evolutionary divergence. We show how Spidey was used to align reference sequences to known genomic sequences and then to the draft human genome, to align mRNAs to gene clusters, and to align mouse mRNAs to human genomic sequence. We compared Spidey to two other spliced alignment programs; Spidey generally performed quite well in a very reasonable amount of time.
journal_name
Genome Resjournal_title
Genome researchauthors
Wheelan SJ,Church DM,Ostell JMdoi
10.1101/gr.195301subject
Has Abstractpub_date
2001-11-01 00:00:00pages
1952-7issue
11eissn
1088-9051issn
1549-5469journal_volume
11pub_type
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