Abstract:
:Trace Recalling is a novel method for deconvoluting double traces that result from simultaneously sequencing two DNA templates. Trace Recalling identifies up to two bases at each position of such a trace. The resulting ambiguity sequence is aligned to the genome, identifying one template sequence. A second template sequence is then inferred from this alignment. This technique makes possible many exciting biological applications. Here we present two such applications, alternate splice finding and elucidation of multiple insertion sites in a random insertional mutagenesis library. Our results demonstrate that RT-PCR followed by Trace Recalling is a more efficient and cost effective way to find alternate splices than traditional methods. We also present a method for mapping double-insertion events in a random insertional-mutagenesis library.
journal_name
Genome Resjournal_title
Genome researchauthors
Tenney AE,Wu JQ,Langton L,Klueh P,Quatrano R,Brent MRdoi
10.1101/gr.5661407subject
Has Abstractpub_date
2007-02-01 00:00:00pages
212-8issue
2eissn
1088-9051issn
1549-5469pii
gr.5661407journal_volume
17pub_type
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