Abstract:
PURPOSE:To determine the temperature-time threshold of local cell death in vivo for thermal therapy in a prostate cancer animal model and to use this value as a benchmark to quantify global tissue injury. MATERIALS AND METHODS:Two studies were designed in the Dunning AT-1 rat prostate tumor hind limb model. For both studies, a wet electrode radiofrequency (RF) probe was used to deliver 40 W of energy for 18 to 62 seconds after a 30-second infusion of hypertonic saline/Hypaque through the RF antenna. Thermal history measurements were obtained in tumors from at least two Fluoroptic probes placed radially 5 mm from the axis of a RF probe and 10 mm below the surface of the tissue. In study 1, the thermal history required for irreversible cell injury was experimentally determined by comparing the predicted injury accumulation (omega) with cell viability at the fluoroptic probe locations using an in vivo-in vitro assay. The omega value was calculated from the measured thermal histories using an Arrhenius damage model. In study 2, RF energy was applied for 40 seconds in all cases. At 1, 3, and 7 days after thermal therapy, triphenyltetrazolium chloride dye (TTC) and histologic analyses were performed to assess global tissue injury within a 5-mm radius from the axis of the RF probe. RESULTS:Study 1 showed that cell survival dropped to 0 for 0.42 < omega < 0.7. This result was the basis for selection of 40 seconds of RF thermal therapy in study 2, which yielded omegaave = 0.5 in the tissue 5 mm from the probe axis. Both TTC and histology analysis showed that sham-treated tissue was not irreversibly injured. However, there was an inherent heterogeneity present in the tumor that accounted for as much as 15% necrosis in control or sham-treated tissue. In contrast, at 1, 3, and 7 days after therapy, significantly less enzyme activity was observed by TCC in thermally treated tissue compared with sham-treated tissue (35 v 85%; P < 0.001). Histologic analysis of thermally treated tissues revealed a gradual increase in the percent of coagulative necrosis (47%-70%) with a concomitant decrease in the percentage of shocked cells (53%-28%). At day 7, <3% viability was observed in treated tumors compared with 90% viability in sham-treated tissue. CONCLUSION:The threshold of cellular injury in vivo corresponded to omega > 0.7 (> or =48 degrees C for 40 seconds). Global tissue injury could be conservatively predicted on the basis of local thermal histories during therapy.
journal_name
J Endouroljournal_title
Journal of endourologyauthors
Bhowmick S,Swanlund DJ,Coad JE,Lulloff L,Hoey MF,Bischof JCdoi
10.1089/089277901750426436subject
Has Abstractpub_date
2001-08-01 00:00:00pages
629-40issue
6eissn
0892-7790issn
1557-900Xjournal_volume
15pub_type
杂志文章abstract:INTRODUCTION:Morcellation of the adenoma after laser enucleation of the prostate (LEP) is both time-consuming and prone to complications. We have designed a novel polyethylene sack (ProSac) to improve the morcellation process following LEP. Both silicone and cadaver models were utilized to evaluate the safety and effic...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2018.0618
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:To determine whether or not temporary drainage is necessary immediately following laparoscopic (lap) and robot-assisted (rob) pyeloplasty (PP). PATIENTS AND METHODS:Of 99 patients undergoing lap PP (n = 23) or rob PP (n = 76) for treatment of ureteropelvic junction obstruction (UPJO), 52 had no drainage, 47 ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2016.0655
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND AND PURPOSE:The gold standard treatment for upper-tract transitional-cell carcinoma is radical nephroureterectomy, but management of the distal ureter is not standardized. Two treatment options to detach the distal ureter are open cystotomy (OC) and excision of a bladder cuff or transurethral incision of the...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2006.0374
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Radiation exposure during medical procedures continues to be an increasing concern for physicians and patients. We determined organ-specific dose rates and calculated effective dose rates during right and left percutaneous nephrolithotomy (PCNL) using a validated phantom model. MATERIALS AND MET...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2011.0178
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND AND PURPOSE:To assess the feasibility and efficacy of percutaneous nephrolithotomy (PCNL) in patients with spinal deformities. PATIENTS AND METHODS:Between 1999 and 2008, eight patients (nine renal units) with spinal deformities underwent 10 PCNLs. Mean stone burden was 372 mm(2) (160-840 mm(2)). Preoperati...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2010.0095
更新日期:2010-07-01 00:00:00
abstract:PURPOSE:To construct, train, and apply an artificial neural network (ANN) system for prediction of different outcome variables of percutaneous nephrolithotomy (PCNL). We calculated predictive accuracy, sensitivity, and precision for each outcome variable. METHODS:During the study period, all adult patients who underwe...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2016.0791
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND AND PURPOSE:As life expectancy continues to increase, we will be faced with the need to counsel older patients on the risks and benefits of undergoing surgery. It is clear that laparoscopic renal surgery has significant benefits over open renal surgery. However, contemporary data on whether these benefits ca...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2007.9885
更新日期:2007-11-01 00:00:00
abstract:PURPOSE:To enable all personnel involved with laparoscopy to obtain cheap and ready practice in a variety of situations. MATERIALS AND METHODS:A number of components are required to practice laparoscopic techniques: a cavity, lighting, video imaging, instruments, operating sutures, etc. and operative tissue. Alternati...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2005.19.748
更新日期:2005-07-01 00:00:00
abstract:INTRODUCTION:The Endockscope combines a smartphone, a battery powered flashlight and a fiberoptic cystoscope allowing for mobile videocystoscopy. We compared conventional videocystoscopy with the Endockscope paired with next generation smartphones in an ex-vivo porcine bladder to evaluate its image quality. MATERIALS ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2018.0275
更新日期:2018-08-01 00:00:00
abstract:: Objective: To evaluate whether reducing the dose of fluoroscopy to ¼ of standard dose during unilateral ureteroscopy for ureteral stone treatment would impact in a reduction of total radiation emitted and whether this strategy would impact operation time, stone-free rate, ...
journal_title:Journal of endourology
pub_type: 杂志文章,随机对照试验
doi:10.1089/end.2018.0722
更新日期:2019-07-01 00:00:00
abstract:INTRODUCTION:Open nephrectomy is associated with significant morbidity. For several years, minimally invasive alternatives have been developed such as laparoscopic nephrectomy or transarterial renal ablation. This paper focuses on the different principles of vaso-occlusion and further improvements of the technique such...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2007.9826
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Conical blunt trocar insertion may eliminate the need for fascial closure (FC) in transperitoneal laparoscopic renal surgery. This concept applies to 12-mm blunt trocar placement through muscular parts of the abdominal wall, relying on muscle splitting and eventual muscle retraction when the troc...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/089277902753483718
更新日期:2002-02-01 00:00:00
abstract:INTRODUCTION:Ureteroscopic laser lithotripsy is becoming the most commonly utilized treatment for patients with urinary calculi. The Holmium:YAG (yttrium aluminum garnet) laser is integral to the operation and is the preferred flexible intracorporeal lithotrite. In recent years, there has been increasing interest in ex...
journal_title:Journal of endourology
pub_type: 杂志文章,评审
doi:10.1089/end.2017.0641
更新日期:2018-01-01 00:00:00
abstract:INTRODUCTION:The da Vinci surgical platform is becoming increasingly available and utilized. Due to the lack of haptic feedback, visual cues are necessary to estimate grip forces and tissue tensions during surgery. We directly measured the grip forces of robotic EndoWrist instruments using the three available da Vinci ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2010.0306
更新日期:2011-03-01 00:00:00
abstract:INTRODUCTION:The gold standard treatment for upper tract urothelial carcinoma (UTUC) is radical nephroureterectomy (RNU). The role of endoscopic resection is limited to low-risk patients. In this study, we present our 30-year experience in the endoscopic management of UTUC. METHODS:In this retrospective study, we iden...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2015.0248
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Recent advances in endoscopy have enabled microscopic imaging of live bladdercancer cells in situ. The newly developed Endocystoscopy system (Olympus Medical Systems Co., Tokyo, Japan) is a flexible digital endoscope that has more than a 450-fold magnifying power on a video monitor. PATIENTS AND...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2006.20.698
更新日期:2006-09-01 00:00:00
abstract::Laser systems used for prostatectomy may be right-angle (sidefiring) devices, which are either refractive or reflective, or contact devices. The tissue physics of these systems differ, and as a result, there are very real differences in the optimal exposure techniques. An understanding of the device being used is esse...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.1995.9.85
更新日期:1995-04-01 00:00:00
abstract:PURPOSE:We evaluated whether epigallocatechin gallate (EGCG), a main constituent of green tea polyphenols, could protect against cellular toxicity by oxalate and whether green tea supplementation attenuates the development of nephrolithiasis in an animal model. MATERIALS AND METHODS:Cells of the NRK-52E line were incu...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2006.20.356
更新日期:2006-05-01 00:00:00
abstract::A trichobezoar with a calculus in the center was diagnosed in a young woman on long-term urethral catheter drainage. Urologists need to be aware of this potentially preventable complication to save patients from significant morbidity. ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/0892779041271562
更新日期:2004-06-01 00:00:00
abstract:PURPOSE:To review the outcome of transperitoneal laparoscopic dismembered pyeloplasty (LDP) in our institution. PATIENTS AND METHODS:From April 2001 to April 2005, 36 men and 34 women underwent LDP for symptomatic and radiologically proved ureteropelvic junction (UPJ) obstruction. A successful outcome was defined clin...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2006.20.1050
更新日期:2006-12-01 00:00:00
abstract:INTRODUCTION:Prestenting of the ureter is commonly performed to allow for passive dilation and better access to the urinary system during subsequent procedures. There is no level 1 evidence on the duration of prestenting and EAU guidelines suggest a 1-2 weeks duration. MATERIALS AND METHODS:Our primary aim is to inves...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2018.0872
更新日期:2019-04-01 00:00:00
abstract:PURPOSE:To define possible predictive factors for success and complications for ureteroscopic holmium laser lithotripsy procedures. PATIENTS AND METHODS:All 105 ureteroscopic holmium laser lithotripsy procedures performed between 1996 and 2005 were analyzed. Data recorded were sex, age, stone size, stone location, com...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2007.0299
更新日期:2008-02-01 00:00:00
abstract:: Introduction: There has been a surge in robotic surgery research publications over the past 20 years. However, to date, there has been no characterization of urology's contribution to the robotic field, and there is a lack of bibliometric literature to guide future investi...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2018.0866
更新日期:2019-05-01 00:00:00
abstract:: Objective: The aim was to assess a new biodegradable and antireflux intraureteral stent (BraidStent®) design in a swine model after ureteral laparoscopic operation. Materials and Methods: A total of 24 female pigs underwent ini...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2019.0493
更新日期:2020-03-01 00:00:00
abstract::Radical nephroureterectomy, including en bloc excision of the ureter with a bladder cuff, is the standard treatment for upper tract transitional-cell carcinoma (TCC). This procedure requires either a flank and lower abdominal incision or an extended flank incision. Laparoscopic surgery for TCC has been used at several...
journal_title:Journal of endourology
pub_type: 杂志文章,评审
doi:10.1089/089277901300189394
更新日期:2001-05-01 00:00:00
abstract::Inability to remove a urethral catheter after radical retropubic prostatectomy is an uncommon complication. We describe removal of a urethral catheter entrapped in vesicourethral anastomotic sutures, which was safely performed endoscopically using a holmium laser. ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2005.19.83
更新日期:2005-01-01 00:00:00
abstract:PURPOSE:Recently, laparoscopic nephropexy has been performed using a transperitoneal approach. We evaluated the efficacy of a retroperitoneoscopic technique for symptomatic nephroptosis. PATIENTS AND METHODS:Three men and two women with right nephroptosis underwent retroperitoneal laparoscopic nephropexy. Their sympto...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/089277903770802344
更新日期:2003-11-01 00:00:00
abstract:BACKGROUND AND PURPOSE:No literature exists examining the risk factors for failure after robot-assisted laparoscopic pyeloplasty for ureteropelvic junction obstruction (UPJO) in children. The goal of this study was to compare the success of robot-assisted pyeloplasty between UPJO kidneys with preoperative renal functio...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2014.0876
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND:Cryptorchidism is a common condition in boys. Approximately 20% of undescended testes are nonpalpable and may be located within the abdominal cavity. Given the potential of these gonads for malignant transformation and infertility, it is essential to determine the presence or absence of a nonpalpable testis....
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/089277901750161719
更新日期:2001-04-01 00:00:00
abstract::Endoscopic treatment of upper-tract transitional-cell carcinoma (TCC) is well established. Nevertheless, many patients still required major ablative surgery. We have applied our experience with laparoscopic nephrectomy to the performance of laparoscopic nephroureterectomy in order to make the management of upper-tract...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.1998.12.139
更新日期:1998-04-01 00:00:00