Abstract:
:Physiological changes of Lactobacillus plantarum (KFRI 815) by high pressure CO2 treatment were investigated to examine the relevance to microbial inactivation. Characteristic properties of the cells measured in this study included salt tolerance, release of UV-absorbing substances, Mg and K ions, proton permeability, glycolysis, H+-ATPase and constitutive enzymes, and dye uptake. The cells treated with high pressure CO2 of 7 MPa at 30 degrees C for 10 min showed the irreversible cellular membrane damages including loss of salt tolerance, leakage of UV-absorbing substances, release of intracellular ions, collapse of proton permeability and uptake of Phloxine B dye. L. plantarum cells after CO2 treatment also exhibited reduced glycolytic activity and inactivation of some constituent enzymes. However, H+-ATPase of the cell membrane maintained its initial specific activity of about 2.50 U/mg protein even though viability of the cells was reduced by several log cycles after high pressure CO2 treatment.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Hon SI,Pyun YRdoi
10.1016/s0168-1605(00)00393-7subject
Has Abstractpub_date
2001-01-22 00:00:00pages
19-28issue
1-2eissn
0168-1605issn
1879-3460pii
S0168-1605(00)00393-7journal_volume
63pub_type
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