Abstract:
:The chromosomal inversion (16)(p13q22), which is associated with the M4-eosinophilia subtype of human acute myeloid leukemia, causes the fusion of two distinct genes. The polypeptide encoded by the chimeric gene, PEBP2p/CBFp-SMMHC, retains the ability to interact with, and dominantly interfere with the function of proteins possessing the Runt homology domain. The Runt protein homologs constitute the DNA binding subunit of the PEBP2/CBF transcription factor. We examined the subcellular localization of PEBP2beta/CBFbeta-SMMHC, as well as that of Runt protein homologs in leukemic cells carrying inversion 16 by immunoblot analysis. A significant amount of the PEBPbeta/CBFbeta-SMMHC protein was recovered from the nuclear fraction along with the Runt protein homologs. Furthermore, some of both polypeptides was retained in the DNA pellet that represents the material remaining after extraction of nuclear fraction with high salt. These observations suggest that the so-called dominant interfering effect of PEBPbeta/CBFbeta-SMMHC on PEBP2/CBF occurs inside the nucleus. In addition, we could detect PEBP2beta/CBFbeta-SMMHC in the cytoplasmic membrane fraction as well. The function of this membrane-located PEBP2beta/CBFbeta-SMMHC, if any, appears to be unrelated to that of Runt protein homologs.
journal_name
Leukemiajournal_title
Leukemiaauthors
Kanto S,Chiba N,Tanaka Y,Fujita S,Endo M,Kamada N,Yoshikawa K,Fukuzaki A,Orikasa S,Watanabe T,Satake Mdoi
10.1038/sj.leu.2401821subject
Has Abstractpub_date
2000-07-01 00:00:00pages
1253-9issue
7eissn
0887-6924issn
1476-5551journal_volume
14pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract::Chronic exposure to high concentrations of benzene can result in the development of myelodysplastic syndrome (MDS) and acute myelogenous leukemia (AML). Studies of patients occupationally exposed to benzene show a pattern of cytogenetic aberrations involving high frequency of loss of all or part of chromosomes 5 and/o...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2400763
更新日期:1997-09-01 00:00:00
abstract::We have used a 4-day MTT colorimetric assay to study drug sensitivity of leucocytes from leukaemia patients and from normal donors. Response to Adriamycin, vincristine, aclacinomycin A, 3'-deamino-3'-morpholino-13-deoxo-10-hydroxycarminomycin (MX2), and melphalan has been determined, together with the effects of the r...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::The AML1/ETO fusion transcript can be detected by reverse transcription polymerase chain reaction (RT-PCR) in patients with t(8;21)-associated acute myeloid leukemia (AML) in long-term complete remission (CR). Quantitation of the amount of the fusion transcript during CR may therefore be more predictive of cure or rel...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401128
更新日期:1998-09-01 00:00:00
abstract::In this report, a Radiation leukemia virus-transformed murine T cell lymphoma is described which is dependent on the interleukin-2 (IL-2) growth factor for proliferation under single cell conditions of growth. It was isolated from a C57BL mouse which had been primed with the Radiation leukemia virus-induced thymoma, C...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract::This phase 1 dose-escalation study evaluated pomalidomide, bortezomib (subcutaneous (SC) or intravenous (IV)) and low-dose dexamethasone (LoDEX) in lenalidomide-refractory and proteasome inhibitor-exposed relapsed or relapsed and refractory multiple myeloma (RRMM). In 21-day cycles, patients received pomalidomide (1-4...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2017.173
更新日期:2017-12-01 00:00:00
abstract::We describe the use and outcomes of allogeneic hematopoietic stem cell transplantation (allo-HSCT) for multiple myeloma (MM) in Europe between January 1990 and December 2012. We identified 7333 patients, median age at allo-HSCT was 51 years (range: 18-78), of whom 4539 (62%) were males. We distinguished three groups: ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2016.101
更新日期:2016-10-01 00:00:00
abstract::A total of 1111 children with acute myeloblastic leukaemia (AML) were treated in four consecutive Berlin-Frankfurt-Münster (BFM) studies from 1978 to 1998. The first cooperative trial AML-BFM 78 established intensive chemotherapy with seven drugs, CNS irradiation and 2-year maintenance, achieving a long-term survival ...
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:10.1038/sj.leu.2403920
更新日期:2005-12-01 00:00:00
abstract::Older adults with acute myeloid leukemia (AML) who are not fit for standard chemotherapy historically have poor outcomes. Approximately 12-15% of older patients with AML harbor isocitrate dehydrogenase 2 (IDH2) gene mutations. Enasidenib is an oral inhibitor of mutant IDH2 proteins. Among 39 patients with newly diagno...
journal_title:Leukemia
pub_type: 杂志文章,多中心研究
doi:10.1038/s41375-019-0472-2
更新日期:2019-11-01 00:00:00
abstract::The mesenchymal stroma has been shown to play a crucial role in the development of multiple myeloma, partly by secretion of interleukin (IL)-6, that serves as a growth factor for myeloma cells. However, it is still unclear which other stromal molecules are involved in the pathogenesis of this disease. We chose, as a m...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402145
更新日期:2001-07-01 00:00:00
abstract::Successful treatment results for MLL-rearranged Acute Lymphoblastic Leukemia (ALL) in infants remain difficult to achieve. Significantly contributing to therapy failure is poor response to glucocorticoids (GCs), like prednisone. Thus, overcoming resistance to these drugs may be a crucial step towards improving prognos...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2013.245
更新日期:2014-04-01 00:00:00
abstract::Of 30 patients with CLL examined with gastroscopy and 18 with additional coloscopy two showed lymphocytic infiltration of the stomach as well as of the large intestine, one of the stomach, and two of the large intestine only. The lymphocytic infiltration seems to depend more on tumor burden and proliferation activity ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::We have previously shown that all-trans retinoic acid (ATRA) increases the number of CFU-GM colonies grown from unseparated human bone marrow cells with crude sources of colony stimulating factors. In this study, we further characterized the effect of ATRA on the growth of CFU-GM stimulated by individual cytokines fro...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401772
更新日期:2000-05-01 00:00:00
abstract::The Wilms' tumor 1 (WT1) gene encodes a transcription factor important for normal cellular development and cell survival. The initial discovery of WT1 as the causative gene in an autosomal-recessive condition identified it as a tumor suppressor gene whose mutations are associated with urogenital disease and the develo...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/sj.leu.2404624
更新日期:2007-05-01 00:00:00
abstract::The mechanism by which aplidine, a marine natural product in early clinical development as an anticancer agent, induces cell growth inhibition and apoptosis has been investigated in the human leukemia cell line MOLT-4. This cell line is characterized not only by the ability to secrete VEGF, but also for the presence o...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402788
更新日期:2003-01-01 00:00:00
abstract::In this study, the abilities of constitutive and conditional forms of the three Raf kinases to abrogate the cytokine dependency of FDC-P1 cells were examined. The constitutively active forms (delta) of all three Raf kinases were fused to the hormone-binding domain of the estrogen receptor (ER), rendering their activit...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401720
更新日期:2000-04-01 00:00:00
abstract::Data on outcome in older (≥70 years) patients with acute promyelocytic leukemia after treatment with arsenic trioxide (ATO) compared with standard chemotherapy (CTX) is scarce. We evaluated 433 patients (median age, 73.4 years) treated either with ATO+ all-trans retinoic acid (ATO/ATRA; n = 26), CTX/ATRA + ATO during ...
journal_title:Leukemia
pub_type: 杂志文章,多中心研究
doi:10.1038/s41375-020-0758-4
更新日期:2020-09-01 00:00:00
abstract::In a European BIOMED-2 collaborative study, multiplex PCR assays have successfully been developed and standardized for the detection of clonally rearranged immunoglobulin (Ig) and T-cell receptor (TCR) genes and the chromosome aberrations t(11;14) and t(14;18). This has resulted in 107 different primers in only 18 mul...
journal_title:Leukemia
pub_type: 杂志文章,多中心研究,评审
doi:10.1038/sj.leu.2403202
更新日期:2003-12-01 00:00:00
abstract::The p210 bcr-abl fusion protein tyrosine kinase oncogene has been implicated in the pathogenesis of chronic granulocytic leukemia (CGL). Specific intracellular functions performed by p210 bcr-abl have recently been delineated. We considered the possibility that p210 bcr-abl may also regulate the abundance of inosine 5...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::We assessed clinical results in 145 patients with chronic myeloid leukaemia in chronic phase who satisfied criteria for interferon-alpha failure and were thus eligible for treatment with imatinib at the Hammersmith Hospital. We used univariate and multivariate analyses to develop a risk score based on features defined...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402996
更新日期:2003-08-01 00:00:00
abstract::The occurrence of leukemia in a gene therapy trial for SCID-X1 has highlighted insertional mutagenesis as an adverse effect. Although retroviral integration near the T-cell acute lymphoblastic leukemia (T-ALL) oncogene LIM-only protein 2 (LMO2) appears to be a common event, it is unclear why LMO2 was preferentially ta...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2404563
更新日期:2007-04-01 00:00:00
abstract::Evidence is presented that human monocytes and acute myeloblastic leukemic (AML) cells contain both high and low affinity binding sites for interleukin-4 (IL-4). On monocytes 183 +/- 132 high affinity binding sites per cell with a Kd of 60 +/- 29 pM and 1500 +/- 600 low affinity receptors with a Kd of 2.3 +/- 0.4 nM (...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::This work represents an update of our experience on mobilization and transplantation of peripheral blood progenitor cells (PBPC) collected during the early recovery phase after chemotherapy in patients with chronic myelogenous leukemia. The collection of Ph-negative precursor cells occurred in 13/19 (68%) patients mob...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::Myelodysplastic syndrome (MDS) in children is often considered as a variant of acute myeloid leukemia (AML) and frequently treated as such. However, there are very few reported data on the outcome following AML treatment. We analyzed 20 consecutive cases of de novo MDS treated in Denmark according to the NOPHO AML pro...
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::Insertional activation of cellular proto-oncogenes by replication-defective retroviral vectors can trigger clonal dominance and leukemogenesis in animal models and clinical trials. Here, we addressed the leukemogenic potential of vectors expressing interleukin-2 receptor common gamma-chain (IL2RG), the coding sequence...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2008.118
更新日期:2008-08-01 00:00:00
abstract::The value of the hypervariable X-linked probe M27 beta for use in the analysis of X-chromosome inactivation patterns in normal blood and bone marrow cells and in the assessment of clonality in acute myeloid leukemia (AML) blast cells has been determined. By electrophoresing samples for 30 h, heterozygosity of the M27 ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract::Dysplastic hematopoiesis is the morphological hallmark of myelodysplastic syndromes. Dysplastic features in one or more lineages are also found frequently in bone marrow aspirates from patients with de novo AML and have been associated with an unfavorable prognosis. We asked whether dyshematopoiesis is an independent ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::Epigenetic deregulation is involved in acute myeloid leukemia (AML) pathogenesis and epigenetic targeting drugs are in clinical trial. Since the first results with histone-deacetylase inhibitors in AML are controversial, novel single and combined treatments need to be explored. It is tempting to combine chromatin-targ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2011.271
更新日期:2012-04-01 00:00:00
abstract::The response of human acute myelogenous leukemia (AML) cells to four different hematopoietic growth factors (granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-1 beta (IL-1beta), interleukin-3 (IL-3), and stem cell factor (SCF)) and the relationship of the proliferative response of the AML cells to...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2400870
更新日期:1997-12-01 00:00:00
abstract::Allogeneic hematopoietic stem cell transplantation (allo-SCT) represents the only curative treatment for patients with myelodysplastic syndrome (MDS), but involves non-negligible morbidity and mortality. Crucial questions in clinical decision-making include the definition of optimal timing of the procedure and the ben...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2017.88
更新日期:2017-11-01 00:00:00
abstract::To determine the frequency and prognostic significance of recently described genetic lesions in pediatric acute lymphoblastic leukemia (ALL), all cases with available leukemic cell samples treated on St Jude Study XII were analyzed by molecular techniques for alterations of the p16, MLL and ETV6 genes. Homozygous p16 ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2400779
更新日期:1997-08-01 00:00:00