Abstract:
:The influence of pH (5.0-8.2), NaCl concentrations (0-6% w/v), and incubation time (0-24 h) on the inhibitory activity of nisin (0-100 I.U./ml) against Listeria monocytogenes (10(3) cfu/ml) was studied using the Doehlert experimental design and was confirmed by kinetic experiments. Predicted values were in agreement with experimental values. Experiments were carried out at 22 degrees C in reconstituted TSB-YE1 broth with or without NaCl. Nisin had an immediate pH-dependent bactericidal effect, which increased with decreasing pH values. In modified TSB-YE1 broth without NaCl, the bactericidal efficacy of nisin (50 I.U./ml) was maximum at pH 6.6, with no L. monocytogenes survivors until 120 h at 22 degrees C. Nisin (50 I.U./ml) action decreased in the presence of NaCl, with a minimal inhibitory effect between 2 and 4%. This partially protective effect was cancelled at higher levels of nisin.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Bouttefroy A,Mansour M,Linder M,Milliere JBdoi
10.1016/s0168-1605(99)00171-3subject
Has Abstractpub_date
2000-03-10 00:00:00pages
109-15issue
1-2eissn
0168-1605issn
1879-3460pii
S0168-1605(99)00171-3journal_volume
54pub_type
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