Abstract:
:We introduced the interleukin-12 (IL-12) gene into mouse renal cell carcinoma (RenCa) cells to develop a tumor vaccine and to examine mechanisms of tumor rejection. IL-12-secreting RenCa (RenCa/IL-12) cells were completely rejected when implanted into syngeneic BALB/c but not athymic nude mice, suggesting that T cells were involved in this antitumor effect. Depletion of natural killer (NK) cells in nude mice did not affect the tumor growth of RenCa/IL-12. The simultaneous injection of mitomycin C-treated RenCa/IL-12 inhibited the tumor growth of parental RenCa injected at a distant site, whereas injection of mitomycin C-treated parental RenCa did not. The antitumor effect of RenCa/IL-12 as a cancer vaccine was induced by CD8+ T cells and NK cells and was inhibited by CD4+ T cells. Although the systemic administration of recombinant IL-18 (rIL-18) alone did not inhibit the tumor growth, it did enhance the cancer vaccine effect of RenCa/IL-12. The combination therapy of RenCa/IL-12 and the systemic administration of rIL-18 retarded even the growth of established tumors. The effector cells of this combination therapy consist not only of CD8+ T cells and NK cells but also of CD4+ T cells. This synergistic cancer vaccine effect of in situ secretion of IL-12 and the systemic administration of rIL-18 may be attributed to a functional change of CD4+ T cells.
journal_name
Cancer Gene Therjournal_title
Cancer gene therapyauthors
Hara I,Nagai H,Miyake H,Yamanaka K,Hara S,Micallef MJ,Kurimoto M,Gohji K,Arakawa S,Ichihashi M,Kamidono Sdoi
10.1038/sj.cgt.7700083subject
Has Abstractpub_date
2000-01-01 00:00:00pages
83-90issue
1eissn
0929-1903issn
1476-5500journal_volume
7pub_type
杂志文章abstract::Cisplatin (DDP)-based strategies are the first-line treatment for cancers; however, resistance to DDP remains a major obstacle to cancer treatment. The current study set out to investigate the effects of microRNA-181c (miR-181c) on the resistance of ovarian cancer cells to DDP. Ovarian cancer-associated miRs as well a...
journal_title:Cancer gene therapy
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journal_title:Cancer gene therapy
pub_type: 临床试验,杂志文章
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journal_title:Cancer gene therapy
pub_type: 已发布勘误
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journal_title:Cancer gene therapy
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journal_title:Cancer gene therapy
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journal_title:Cancer gene therapy
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journal_title:Cancer gene therapy
pub_type: 杂志文章
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journal_title:Cancer gene therapy
pub_type: 杂志文章
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journal_title:Cancer gene therapy
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journal_title:Cancer gene therapy
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journal_title:Cancer gene therapy
pub_type: 杂志文章
doi:10.1038/sj.cgt.7700488
更新日期:2002-08-01 00:00:00
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journal_title:Cancer gene therapy
pub_type: 杂志文章,撤回出版物
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journal_title:Cancer gene therapy
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journal_title:Cancer gene therapy
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journal_title:Cancer gene therapy
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