Abstract：
:There is increasing evidence that HOX homeobox genes play a role in leukemogenesis. Recent studies have demonstrated that enforced co-expression of HOXA9 and MEIS1 in murine marrow leads to rapid development of myeloid leukemia, and that these proteins exhibit cooperative DNA binding. However, it is unclear whether co-activation of HOXA9 and MEIS genes is a common occurrence in human leukemias. We surveyed expression of HOXA9 and MEIS1 in 24 leukemic cell lines and 80 patient samples, using RNase protection analyses and immunohistochemistry. We demonstrate that the expression of HOXA9 and MEIS1 in leukemia cells is uniquely myeloid, and that these genes are commonly co-expressed in myeloid cell lines and in samples of acute myelogenous leukemia (AML) of all subtypes except in promyelocytic leukemia. While HOXA9 is expressed in most cases of chronic myelogenous leukemia, MEIS1 is weakly expressed or not at all. Immunohistochemical staining of selected AML samples showed moderate to high levels of HOXA9 protein, primarily cytoplasmic, in leukemic myeloblasts, with weaker and primarily nuclear staining for MEIS1. These data support the concept that co-activation of HOXA9 and MEIS1 is a common event in AML, and may represent a common pathway of many different oncogenic mutations.
journal_name
Leukemiajournal_title
Leukemiaauthors
Lawrence HJ,Rozenfeld S,Cruz C,Matsukuma K,Kwong A,Kömüves L,Buchberg AM,Largman Cdoi
10.1038/sj.leu.2401578subject
Has Abstractpub_date
1999-12-01 00:00:00pages
1993-9issue
12eissn
0887-6924issn
1476-5551journal_volume
13pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract：:In order to assess the proliferative capacity of leukemic subpopulations and to know whether it can be related to the stage of maturation, the expression of two surface antigens identifying distinct steps of leukocyte differentiation (CD15 and CD34) was studied by flow cytometry in correlation with DNA content in 16 c...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Myeloproliferative neoplasms with myelofibrosis (MPN-MF) demonstrate constitutive activation of Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling that responds to treatment with the JAK1 and 2 kinase inhibitor (JAKi) ruxolitinib. However, MPN-MF often progresses (~20%) to secondary acu...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2016.260
更新日期：2017-03-01 00:00:00
abstract：:Response rates of 20% were achieved when interferon alpha (IFN) was applied as single agent treatment for multiple myeloma. A synergistic activity was observed when IFN was combined with chemotherapeutic agents in vitro and in vivo. These observations led to a series of randomized trials comparing chemotherapy alone w...
journal_title：Leukemia
pub_type： 杂志文章,评审
doi：
更新日期：1997-12-01 00:00:00
abstract：:T-cell acute lymphoblastic leukemia (T-ALL) is associated with chromosomal aberrations characterized by juxtaposition of proto-oncogenes to T-cell receptor gene loci (TCR), resulting in the deregulated transcription of these proto-oncogenes. Here, we describe the molecular characterization of a novel chromosomal aberr...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2403619
更新日期：2005-02-01 00:00:00
abstract：:ET is a chronic myeloproliferative disorder rarely evolving into AML, sometimes preceded by a myelodysplastic syndrome (MDS). Such transformations mostly occur in patients treated with radiophosphorous ((32)P) or alkylating agents, especially busulfan. Recently, concern has also arisen about the long-term safety of hy...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2402638
更新日期：2002-10-01 00:00:00
abstract：:Myeloproliferative neoplasms (MPNs) are clonal stem cell disorders characterized by chronic proliferation of hematopoietic progenitors. We studied the telomere length (TL) of 335 MPN patients and 93 gender- and age-matched controls using a quantitative PCR method (relative TL calculated as the ratio of the amount of t...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2008.319
更新日期：2009-02-01 00:00:00
abstract：:Acute myeloid leukemia (AML) involves a block in terminal differentiation of the myeloid lineage and uncontrolled proliferation of a progenitor state. Using phorbol myristate acetate (PMA), it is possible to overcome this block in THP-1 cells (an M5-AML containing the MLL-MLLT3 fusion), resulting in differentiation to...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2009.246
更新日期：2010-02-01 00:00:00
abstract：:The expression of myeloid surface markers was investigated in 41 cases of untreated adult acute lymphoblastic leukemia (ALL). Nineteen cases (46%) reacted with at least one myeloid monoclonal antibody (CD15 in 16 cases, CD13 in 10 cases, CD14 in five cases, and CD33 in four cases). Double-staining confirmed the coexpr...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:The requirement that leukemic Gata1 mutations be present in cells harboring trisomy 21 led to the discovery that overexpression of ERG drives aberrant megakaryopoiesis. Given that constitutive PI3K/AKT signaling is a frequent component of hematologic malignancies and the relationship between AKT and Notch in this line...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2013.33
更新日期：2013-06-01 00:00:00
abstract：:GM-CSF transgenic mice were crossed with mice with homozygous inactivation of the gene encoding the common beta chain (beta c) of the GM-CSF receptor to produce mice with constitutively elevated GM-CSF levels but no high-affinity GM-CSF receptors. GM-CSF transgenic beta c -/- mice had exceptionally elevated serum GM-C...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2400926
更新日期：1998-03-01 00:00:00
abstract：:Thirty adult patients with CMML were evaluated to determine prognostic factors that might have an impact on conversion to acute leukemia and survival. Neither leukocyte count nor monocyte count correlated with survival. The median survival for all 30 cases was 41 months. Patients with less than 5% marrow blasts had a ...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:Approximately 25% of acute leukemias of the B-cell lineage demonstrate more than two rearranged immunoglobulin heavy chain genes when examined by Southern blot analysis. The origin of the extra bands was investigated by molecular cloning and sequencing of four rearranged genes from one patient's leukemic cells. All fo...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:The c-kit proto-oncogene encodes a transmembrane receptor with a tyrosine kinase internal domain. C-kit has been mapped to the W locus in the mouse, and the gene encoding the ligand has been shown to be the product of the murine SI locus. Previous genetic studies have shown that the murine W and SI loci play important...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Reverse transcriptase-polymerase chain reaction is a recent technique in the diagnosis and assessment of minimal residual disease of acute promyelocytic leukemia, by amplification, of the different PML-RARalpha transcripts resulting from the t(15;17) translocation. The main issues addressed by the Second Workshop on P...
journal_title：Leukemia
pub_type：
doi：
更新日期：1996-02-01 00:00:00
abstract：:We have cloned two genes for cell surface molecules, capable of delivering the intracellular signals, which are modulated for their expression by Tax. One is the gamma chain of the interleukin-2 (IL-2) receptor which is suggested to be critical for IL-2-dependent growth of human T-cell leukemia virus type I (HTLV-I) i...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:HOX homeobox proteins are key oncogenic drivers in hematopoietic malignancies. Here we demonstrate that HOXA1, HOXA6 and predominantly HOXA9 are able to induce the production of insulin-like growth factor 1 (Igf1). In chromatin immunoprecipitations, HOXA9 bound directly to the putative promoter and a DNase-hypersensit...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2014.287
更新日期：2015-04-01 00:00:00
abstract：:A total of 1111 children with acute myeloblastic leukaemia (AML) were treated in four consecutive Berlin-Frankfurt-Münster (BFM) studies from 1978 to 1998. The first cooperative trial AML-BFM 78 established intensive chemotherapy with seven drugs, CNS irradiation and 2-year maintenance, achieving a long-term survival ...
journal_title：Leukemia
pub_type： 临床试验,杂志文章
doi：10.1038/sj.leu.2403920
更新日期：2005-12-01 00:00:00
abstract：:Retinoic acid receptor (RA) heterodimer (RAR/RXR) activities have been shown to be repressed by transcriptional co-repressor, SMRT/N-CoR, in the absence of the ligand while upon all-trans retionic acid (ATRA) treatment, SMRT/N-CoR is dissociated from RARalpha leading to gene expression by the recruitment of transcript...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2401643
更新日期：2000-01-01 00:00:00
abstract：:The clinical and cell growth characteristics of 11 children with monosomy 7 presenting as preleukemia (eight cases) or acute nonlymphoblastic leukemia (three cases) were studied. Anemia was common to all patients, with nine showing leukocytosis, seven thrombocytopenia, and one thrombocytosis. There was a striking pred...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:The proto-oncogene c-Jun has been implicated in the control of cell proliferation and differentiation and more recently in the regulation of apoptosis. We have previously reported the involvement of c-Jun in the erythroid differentiation block in murine erythroleukemia (MEL) cells. As reported here, we investigated th...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2402350
更新日期：2002-02-01 00:00:00
abstract：:Retinoids have significant antiproliferative effect against chronic myelogenous leukemia (CML) cells in vitro. We conducted a pilot study to investigate the clinical effect of all-trans retinoic acid (ATRA) in patients with CML. Thirteen patients with Philadelphia chromosome (Ph)-positive CML in late chronic phase (n=...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2400682
更新日期：1997-07-01 00:00:00
abstract：:Imatinib is a highly effective therapy for chronic phase-chronic myeloid leukaemia (CP-CML) patients; however, responses to frontline imatinib are variable. The human organic cation transporter 1 (OCT1; SLC22A1) has been reported to be the main influx transporter involved in imatinib uptake into CML cells. Furthermore...
journal_title：Leukemia
pub_type： 杂志文章,评审
doi：10.1038/leu.2015.170
更新日期：2015-10-01 00:00:00
abstract：:Modern therapy for pediatric acute lymphoblastic leukemia (ALL) is based on the principle of risk stratification. One of the most important laboratory features used to accurately risk stratify patients is the presence of specific chromosomal translocation within the leukemic blasts. In this paper, we describe a multip...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2401224
更新日期：1998-12-01 00:00:00
abstract：:HTLV-1 is an important factor involved in various diseases including adult T-cell leukemia/lymphoma and HTLV-1 associated myelopathy/tropical spastic paraparesis. Amount of HTLV-1 provirus integrated in human peripheral blood mononuclear cells might be a candidate for a risk factor in the manifestation of HTLV-1 assoc...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:Lack of apoptosis has been linked to prolonged survival of malignant B cells expressing bcl-2. The aim of the present study was to analyze the amount of bcl-2 protein expressed along normal human B-cell maturation and to establish the frequency of aberrant bcl-2 expression in B-cell malignancies. In normal bone marrow...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2403231
更新日期：2004-03-01 00:00:00
abstract：:Schedule dependency of idarubicin (Ida) and doxorubicin (Dox) toxicity was investigated in vitro using the K562 human leukemia cell line. For Dox, repeated exposure to the IC30 (d x 3) resulted in comparable survival as single exposure to the total accumulative dose (20%). For Ida, repeated exposure to the IC30 (d x 3...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:To determine the frequency and prognostic significance of recently described genetic lesions in pediatric acute lymphoblastic leukemia (ALL), all cases with available leukemic cell samples treated on St Jude Study XII were analyzed by molecular techniques for alterations of the p16, MLL and ETV6 genes. Homozygous p16 ...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2400779
更新日期：1997-08-01 00:00:00
abstract：:The aim of this study was to collect prospectively unselected, population-based data on young adults with acute myeloid leukaemia (AML) over a 9-year period and to evaluate the impact on survival of the introduction of allogeneic transplantation performed in first remission. The population within the Northern Region o...
journal_title：Leukemia
pub_type： 临床试验,杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:We evaluated the synergistic activity of AS101 (ammonium trichloro-(dioxoethylene-0-0')-tellurate) with the protein kinase C (PKC) activators, Bryostatin-1 and phorbol-12-myristate-13-acetate (PMA), on human myelocytic leukemia cell differentiation in vitro, and in a mouse model. Use of AS101 with Bryostatin-1 or with...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2404746
更新日期：2007-07-01 00:00:00
abstract：:Adhesion molecules and stromal cell-derived factor-1 (SDF-1)/CXCR4 signaling play key role in homing and mobilization of hematopoietic progenitor (HPC) and hematopoietic cancer clonogenic cells (HCC). High expression of VLA-4 is required for homing of HPC and HCC, whereas downregulation of these molecules is required ...
journal_title：Leukemia
pub_type： 杂志文章,评审
doi：10.1038/sj.leu.2403173
更新日期：2004-01-01 00:00:00
