Vasal reanastomosis using fibrin glue combined with sutures: which combination of sutures in a delayed protocol? Experimental study in rats.

Abstract:

OBJECTIVES:The actual number of transmural sutures needed to ensure a successful fibrin-glued vasovasostomy is a key study parameter of the few experimental works already published. The present work was done to evaluate fibrin-glued vasovasostomy in rats in combination with only 2 transmural sutures. We compared the results to our previous study in which we demonstrated the efficiency of a combination of the use of fibrin glue with 3 sutures in comparison with a conventional microsurgical technique. MATERIALS AND METHODS:Twenty Sprague-Dawley rats underwent bilateral vasectomy followed 2 weeks later by bilateral vasovasostomy using fibrin glue combined with 2 transmural sutures. Each animal was sacrificed 7 weeks postoperatively after a 3-week mating period with a Sprague-Dawley female rat, the vasal specimens were evaluated for sperm granuloma formation. Mean operative time and fertility rates were recorded. RESULTS:The combination of fibrin glue with 2 transmural sutures gave evidence of less successful performances than the combination with 3 transmural sutures and the conventional microsurgical technique for all parameters evaluated but the mean operative time. CONCLUSION:Our study underlines the need for a third transmural suture placed 120 degrees apart from the others when performing a fibrin glue delayed vasovasostomy. This allows a better vas lumen opening at the anastomotic site and therefore a more efficient vasal anastomosis in a delayed protocol.

journal_name

Eur Urol

journal_title

European urology

authors

Vankemmel O,de la Taille A,Rigot JM,Burnouf T,Mazeman E

doi

10.1159/000019567

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

318-22

issue

3

eissn

0302-2838

issn

1873-7560

pii

eur33318

journal_volume

33

pub_type

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