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Simultaneous measurement of nineteen binding constants of peptides to vancomycin using affinity capillary electrophoresis-mass spectrometry.

Abstract：

:On-line affinity capillary electrophoresis-electrospray ionization-mass spectrometry (ACE-MS) was used for the simultaneous measurement of multiple binding constants of an all-D-tetrapeptide library to the model receptor, vancomycin. Determination of Kd values for the 19 peptides of the form Fmoc-DXYA is demonstrated. The data are compared with the results obtained for individual compounds using ACE-UV, and good correlation between the two detection methods is shown. Simultaneous determination of multiple Kd values by ACE-MS is achieved in one set of experiments, whereas only one Kd value can be obtained by ACE-UV during the same time. ACE-MS measures multiple binding constants in solution in a fast and reliable manner using femtomole amounts of samples.

journal_name

J Med Chem

journal_title

Journal of medicinal chemistry

authors

Dunayevskiy YM,Lyubarskaya YV,Chu YH,Vouros P,Karger BL

doi

10.1021/jm970578s

subject

Has Abstract

pub_date

1998-03-26 00:00:00

pages

1201-4

issue

7

eissn

0022-2623

issn

1520-4804

pii

jm970578s

journal_volume

41

pub_type

杂志文章

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