Abstract:
:Experimental tumors of the central nervous system were investigated with antibodies to quinolinate to assess the cellular distribution of this endogenous neurotoxin. In advanced F98 and RG-2 glioblastomas and E367 neuroblastomas in the striatum of rats, variable numbers of quinolinate immunoreactive cells were observed in and around the tumors, with the majority being present within tumors, rather than brain parenchyma. The stained cells were morphologically variable, including round, complex, rod-shaped, and sparsely dendritic cells. Neuroblastoma and glioma cells were unstained, as were neurons, astrocytes, oligodendrocytes, ependymal cells, endothelial cells, and cells of the choroid plexus and leptomeninges. Glial fibrillary acidic protein immunoreactivity was strongly elevated in astrocytes surrounding the tumors. Dual labeling immunohistochemistry with antibodies to quinolinate and glial fibrillary acidic protein demonstrated that astrocytes and the cells containing quinolinate immunoreactivity were morphologically disparate and preferentially distributed external and internal to the tumors, respectively, and no dual labeled cells were observed. Lectin histochemistry with Griffonia simplicifolia B4 isolectin and Lycopersicon esculentum lectin demonstrated numerous phagocytic macrophages and reactive microglia in and around the tumors whose distribution was similar to that of quinolinate immunoreactive cells, albeit much more numerous. Dual labeling studies with antibodies to quinolinate and the lectins demonstrated partial codistribution of these markers, with most double-labeled cells having the morphology of phagocytes. The present findings suggest the possibility that quinolinate may serve a functional role in a select population of inflammatory cell infiltrates during the immune response to brain neoplasms.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Moffett JR,Els T,Espey MG,Walter SA,Streit WJ,Namboodiri MAdoi
10.1006/exnr.1996.6365subject
Has Abstractpub_date
1997-04-01 00:00:00pages
287-301issue
2eissn
0014-4886issn
1090-2430pii
S0014-4886(96)96365-7journal_volume
144pub_type
杂志文章abstract::Traumatic brain injury (TBI) in animals and man generates widespread axonal injury characterized by focal axolemmal permeability changes, induction of calpain-mediated proteolysis, and neurofilament side-arm modification associated with neurofilament compaction (NFC) evolving to axonal disconnection. Recent observatio...
journal_title:Experimental neurology
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abstract::We studied electrophysiological and morphological properties of the Aalpha- and Abeta-fibers in the regenerating sciatic nerve to establish whether these fiber types regenerate in numerical proportion and whether and how the electrophysiological properties of these fiber types are adjusted during regeneration. Compoun...
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1993.1005
更新日期:1993-01-01 00:00:00
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journal_title:Experimental neurology
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更新日期:1999-05-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.12.006
更新日期:2007-04-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2009.03.002
更新日期:2009-06-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2015.12.006
更新日期:2016-11-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.06.004
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/s0014-4886(05)80015-9
更新日期:1990-09-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1999.7162
更新日期:1999-10-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90184-7
更新日期:1988-03-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:1986-04-01 00:00:00
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1999.7074
更新日期:1999-07-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:1998-12-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:1985-08-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
abstract::Ubiquitin (Ub) C-terminal hydrolase L1 (UCHL1) is a multifunctional protein that is expressed in neurons throughout brain at high levels. UCHL1 deletion is associated with axonal degeneration, progressive sensory motor ataxia, and premature death in mice. UCHL1 has been hypothesized to play a role in the pathogenesis ...
journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:2021-02-01 00:00:00
abstract::Posttraumatic inflammation has been implicated in secondary tissue damage after spinal cord injury (SCI). Tumor necrosis factor-alpha (TNF-alpha) is a key inflammatory mediator that is increasingly expressed after SCI. The effect of TNF-alpha is mediated through its receptors TNFR1 (p55) and TNFR2 (p75). However, whet...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/s0014-4886(03)00135-3
更新日期:2003-10-01 00:00:00
abstract::Sympathetic axons have been shown to invade the rat hippocampal formation after lesions of the septohippocampal (cholinergic) projection. To test the generality of this type of transmitter-specific sprouting within the central nervous system, lesions were placed within the horizontal limb of the diagonal band in order...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(84)90235-8
更新日期:1984-05-01 00:00:00
abstract::The loss or injury of neurons associated with oxidative and nitrosative redox stress plays an important role in the onset of various neurodegenerative diseases. Specifically, nitric oxide (NO), can affect neuronal survival through a process called S-nitrosylation, by which the NO group undergoes a redox reaction with ...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
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更新日期:2012-11-01 00:00:00
abstract::Autonomic dysreflexia is a condition of episodic hypertension that develops after spinal cord injury (SCI). We previously showed that a two-day anti-inflammatory treatment with an anti-CD11d integrin monoclonal antibody (mAb), soon after SCI in rats, reduced the magnitude of dysreflexia for at least 6 weeks. Effects o...
journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:2005-08-01 00:00:00
abstract::The neural substrate underlying the aversive effects induced by ethanol abstinence is still unclear. One candidate for such effects is the dorsal periaqueductal gray (dPAG), a core structure of the brain aversion system. The main aim of this study is to examine the role of the dPAG as a possible locus of the aversive ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.02.004
更新日期:2006-07-01 00:00:00