Abstract:
:The presynaptic regulation of amino acid release from nerve terminals was investigated using synaptosomes prepared from the rat spinal cord. The basal releases of endogenous glutamate (Glu), aspartate (Asp), and gamma-amino-butyric acid (GABA) were 34.6, 21.5, and 10.0 pmol/min/mg of protein, respectively. Exposure to a depolarizing concentration of KCl (30 mM) evoked 2.7-, 1.5-, and 2.9-fold increases in Glu, Asp, and GABA release, respectively. Clonidine reduced the K(+)-evoked overflow of Glu to 56% of the control overflow with a potency (IC50) of 17 nM, but it did not affect K(+)-evoked overflow of Asp, GABA, and their basal releases. Similarly, noradrenaline inhibited the K(+)-evoked overflow of Glu, although phenylephrine and isoproterenol showed no effect. The inhibitory effect of clonidine was counteracted by alpha 2-adrenoceptor antagonists, rauwolscine, yohimbine, and idazoxan, regardless of the imidazoline structures. Because Glu is considered a neurotransmitter of primary afferents that transmit both nociceptive and nonnociceptive stimuli in the spinal cord, these data suggest that part of Glu release may be regulated by the noradrenergic system through alpha 2 adrenoceptors localized on the primary afferent terminals.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Kamisaki Y,Hamada T,Maeda K,Ishimura M,Itoh Tdoi
10.1111/j.1471-4159.1993.tb03180.xsubject
Has Abstractpub_date
1993-02-01 00:00:00pages
522-6issue
2eissn
0022-3042issn
1471-4159journal_volume
60pub_type
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pub_type: 杂志文章
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journal_title:Journal of neurochemistry
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
doi:10.1046/j.1471-4159.2001.00159.x
更新日期:2001-03-01 00:00:00
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journal_title:Journal of neurochemistry
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
doi:10.1111/j.1471-4159.1993.tb09793.x
更新日期:1993-11-01 00:00:00
abstract::[3H]Ouabain binding was studied in sections of rabbit somatosensory cortex by quantitative autoradiography and in rabbit brain microsomal membranes using a conventional filtration assay. KD values of 8-12 nM for specific high-affinity binding of [3H]ouabain were found by both methods. High-affinity binding was not uni...
journal_title:Journal of neurochemistry
pub_type: 杂志文章
doi:10.1111/j.1471-4159.1989.tb10916.x
更新日期:1989-01-01 00:00:00