Abstract:
:The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering. The bioactivity of encapsulated cells inside pure alginate hydrogel is limited by its relatively inertness. Combining short nanofibers within a hydrogel serves as a promising method to develop a cell friendly environment mimicking the extracellular matrix. In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation. Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels. The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue. For cell encapsulation, OE-MSCs were used as a new hope for neuronal regeneration due to their neural crest origin. Resazurin analyses and LIVE/DEAD staining confirmed that the composite hydrogels containing M.SNFs can preserve the cell viability after 7 days. Moreover, the proliferation rate was enhanced in M.SNF/hydrogels compared to alginate hydrogel. The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Karimi S,Bagher Z,Najmoddin N,Simorgh S,Pezeshki-Modaress Mdoi
10.1016/j.ijbiomac.2020.11.199subject
Has Abstractpub_date
2021-01-15 00:00:00pages
796-806eissn
0141-8130issn
1879-0003pii
S0141-8130(20)35138-2journal_volume
167pub_type
杂志文章abstract::Sodium houttuyfonate analogs (SHAs), CH(3)-(CH(2))(n)-CO-CH(2)-CH(OH)SO(3)Na, (n=6-14) were synthesized and their molecular interactions with renin and angiotensin I converting enzyme (ACE) studied using fluorescence quenching techniques. Unlike renin, inhibition of ACE activity was not directly proportional to the al...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2007.03.004
更新日期:2007-08-01 00:00:00
abstract::The exploration of new sources of L-asparaginase with low glutaminase activity is of great interest in both medical and food applications. In the current study, a novel L-asparaginase gene (CobAsnase) from halotolerant Cobetia amphilecti AMI6 was cloned and over-expressed in Escherichia coli. The enzyme had a molecula...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.10.258
更新日期:2020-01-15 00:00:00
abstract::The solvent accessible surface area (ASA) of the polysaccharides, namely (i) carrageenan (1CAR); (ii) agarose (1AGA); (iii) guaran (GUR); (iv) capsular polysaccharide (1CAP); and (v) hyaluronan (1HUA), have been computed using the solvent accessibility technique of Lee and Richards. The results show that the average v...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(00)00158-6
更新日期:2001-01-10 00:00:00
abstract::Bicalutamide (BCT), a drug used in the treatment of prostate cancer, antagonises the actions of androgens, at the receptor level, thereby inhibiting the growth of prostate tumours. Alpha-2-macroglobulin (α2M), a pan-proteinase inhibitor, inhibits proteinase, regardless of specificity and catalytic mechanism. α2M is de...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.08.117
更新日期:2018-12-01 00:00:00
abstract::Comparative studies of leafhopper mitochondrial genomes suggest that genome rearrangements are very rare in these insects but that nucleotide sequences are informative of phylogenetic relationships at various levels of divergence. A recent study reported that tRNA cluster trnW-trnC-trnY is rearranged to trnC-trnW-trnY...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.049
更新日期:2019-02-01 00:00:00
abstract::Sucrose phosphorylase (SPase, EC2.4.1.7) is a promising transglycosylation biocatalyst used for producing glycosylated compounds that are widely used in the food, cosmetics, and pharmaceutical industries. In this study, a recombinant SPase from the Thermobacillus sp. ZCTH02-B1 (rTSPase), which was previously reported ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2021.01.115
更新日期:2021-01-19 00:00:00
abstract::Due to the limited options for topical management of skin cancer, this study aimed at developing and evaluating nanoemulsions (NE) for topical delivery of the cytotoxic agent piplartine (piperlongumine). NEs were modified with chitosan or sodium alginate, and the effects on the physicochemical properties, piplartine d...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.09.167
更新日期:2020-12-15 00:00:00
abstract::The present investigation determines the extraction and characterization of seed galactomannans from the Leguminosae taxa of Bauhinia vahlii, Delonix elata, and Peltophorum pterocarpum. The seed galactomannans presented a total yield of 30.82, 24.01 and 25.25% with a Man/Gal ratios of 4.21:1, 2.55:1 and 3.03:1 for B. ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.05.080
更新日期:2019-08-01 00:00:00
abstract::In this present study, ternary nanocomposite films of chitosan/starch/halloysite nanotubes (ChSHT) were fabricated by solution casting process. The ternary nanocomposite films were characterized by using various techniques such as Fourier transform infrared (FTIR), X-ray Diffraction (XRD), water contact angle, water a...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.01.047
更新日期:2019-04-15 00:00:00
abstract::The purpose of the present study was to synthesize gum kondagogu-g-poly(N-vinyl-2-pyrrolidone) and to evaluate its mucoadhesive properties. UV-assisted graft co-polymerization of N-vinyl-2-pyrrolidone on gum kondagogu was carried out employing three-factor, three-level central composite experimental designs. It was ob...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.07.009
更新日期:2012-12-01 00:00:00
abstract::An emerging paradigm in wound healing techniques is that a tissue-engineered skin substitute offers an alternative approach to create functional skin tissue. Here we developed a fish collagen/alginate (FCA) sponge scaffold that was functionalized by different molecular weights of chitooligosaccharides (COSs) with the ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.08.038
更新日期:2015-11-01 00:00:00
abstract::(1-->3)-beta-D-Glucans represent highly conserved structural components of cell walls in yeast, fungi, or seaweed. However, it is still unknown how they mediate their effects. The aim of this study was to evaluate both intraperitoneal and oral application of seaweed-derived (1-->3)-beta-D-glucan Phycarine. Phycarine s...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2006.08.009
更新日期:2007-03-10 00:00:00
abstract::To investigate the antitumor and antimetastatic polysaccharide from the mature leaves of green tea, GTE-II was purified using size exclusion chromatography. GTE-II consisted of 15 different sugars including rarely observed sugars such as 2-O-methyl-fucose, 2-O-methyl-xylose, apiose, aceric acid, 3-deoxy-d-manno-2-octu...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.02.043
更新日期:2017-06-01 00:00:00
abstract::A multi-functionalized poly ether sulfone (PES)/ chitosan (CS)/Fe3O4-NH2-SH nanofiber was applied for single and simultaneous removal of Pb(II) and Ni(II) ions from water systems. The structure of nanofibers was evaluated using FTIR, FESEM, BET, BJH, TGA, EDX and XPS analyses. The characterization results showed that ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.017
更新日期:2020-04-01 00:00:00
abstract::Hypoxia inducible factor 1 alpha (HIF-1α) is a key regulator of the genes involved in the cellular response to hypoxia. This study aims to determine the HIF-1α gene polymorphism and its association with protein expression in diabetic subjects with and without diabetic foot ulcers (DFU). A total of 529 patients with T2...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.08.011
更新日期:2018-11-01 00:00:00
abstract::A novel nucleoside 2'-deoxyribosyltransferase (LhNDT) from Lactobacillus hilgardii ZJS01 was cloned and expressed in Escherichia coli BL21 (DE3). The amino acid sequence of LhNDT displayed the highest identity of 26% to other reported NDTs. LhNDT was a monomer as revealed by gel filtration chromatography with or witho...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.08.104
更新日期:2018-01-01 00:00:00
abstract::The purpose of this study is to clarify that the amino acid residues (Asp62 and Arg193) are responsible for the activity and stability of arginine kinase (AK). The amino acid residues Asp62 (D62) and Arg193 (R193) are strictly conserved in monomeric AKs and form an ion pair in the transition state analogue complex. In...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.05.022
更新日期:2011-10-01 00:00:00
abstract::Small angle X-ray scattering (SAXS) and atomic force microscopy (AFM) measurements have been shown to be consistent with the presence of nanofibrils in the cocoon silk of Bombyx mori and the dragline silk of Nephila clavipes. The transverse dimensions and correlation lengths range from > 59 to 220 nm and in the axial ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(99)00024-0
更新日期:1999-03-01 00:00:00
abstract::A new pH-sensitive drug carrier based on alginate (Alg), chitosan (Cs), and kappa-carrageenan (kC) marine biopolymers was developed, comprising a dual-layered polyelectrolyte complexes microbead structure. Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and scanning electron microsco...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.03.225
更新日期:2019-07-01 00:00:00
abstract::This review aims to highlight the bioprocessing strategies behind one of the most commercially produced enzymes in the biotechnological market, pectinase. Significant steps in the upstream processing of this enzyme include its source, the selection of the substrate, the reaction conditions and the overall reactor desi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2020.06.224
更新日期:2020-11-01 00:00:00
abstract::In this study, a biocomposite of chitosan/poly vinyl alcohol/activated carbon was synthesized and used for hexavalent chrome removal from aqueous solution. The synthesized adsorbent was characterized by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) analysis. The effect of important variables...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.044
更新日期:2020-03-15 00:00:00
abstract::Biodegradable flocculants of Gum ghatti (Gg) with acrylamide (AAm) were prepared through graft co-polymerization technique using potassium persulphate (KPS)-ascorbic acid (ABC) redox pair as initiator and N,N'-methylene-bis-acrylamide (MBA) as a crosslinker. Gg-cl-poly(AAm) was found to exhibit pH and temperature resp...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.03.045
更新日期:2013-07-01 00:00:00
abstract::In recent years, an enormous number of naturally occurring biological macromolecules has been reported worldwide due to its antibacterial and anticancerous potential. Among them, in this study, the copper containing respiratory protein namely haemocyanin (HC) was isolated from the haemolymph of mud crab Scylla serrata...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.228
更新日期:2020-12-01 00:00:00
abstract::Four polysaccharides, namely COP-1, COP-2, COP-3 and COP-4, were ultrafiltrated from crud Camellia oleifera seed cake polysaccharides (COP-c), purified, and characterized, including the determination of antioxidant and antiproliferative activities. Their molecular weights were 7.9, 36, 83 and 225kDa, respectively. All...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.06.067
更新日期:2016-10-01 00:00:00
abstract::Biodegradable polymer composites based on poly(ɛ-caprolactone) (PCL) and poly(lactic acid) (PLA) fibers with diameters of 18, 26, 180 μm were prepared by melt compounding. The PLA fiber content in the composites was constant at 20% by weight. The effects of fibers with different diameters on the physical properties an...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.01.059
更新日期:2015-05-01 00:00:00
abstract::An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG). The results indicated that the degradation efficiency increased with the reduced solution...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.10.161
更新日期:2018-02-01 00:00:00
abstract::A novel biopolymer-based superporous hydrogel (SPH) was synthesized through chemical crosslinking by graft copolymerization of 2-hydroxyethyl methacrylate (HEMA) and acrylic acid (AA) on to xanthan gum (XG) via redox initiator system of ammonium persulfate (APS) and N, N, N', N'-tetramethylethylenediamine (TMED), in t...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2009.07.007
更新日期:2009-11-01 00:00:00
abstract::Cataract, the major cause of blindness worldwide occurs due to the misfolding and aggregation of the protein crystallin, which constitute a major portion of the lens protein. Other than the whole protein crystallin, the peptide sequences generated from crystallin as a result of covalent protein damage have also been s...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.09.112
更新日期:2020-11-15 00:00:00
abstract::In this study, in order to evaluate the influences of drying methods on the chemical structures and bioactivities of polysaccharides from okra (OPPs), four drying methods, including microwave drying at 400 W, 600 W, and 800 W, freezing drying, hot air drying, and vacuum drying, were applied to dry okra fruits. Six dif...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.10.073
更新日期:2020-03-15 00:00:00
abstract::Existing therapies yield low drug encapsulation or accumulation in the lungs, hence the site-specific drug delivery remains the challenge for tuberculosis. Lately, dry powder inhalers (DPIs) are showing promising drug deposition in the deeper lung tissues. Biocompatible polymers with the ability to naturally recognize...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.10.192
更新日期:2020-12-15 00:00:00