Sequence analysis of 16S rDNA, gyrB and alkB genes of plant-associated Rhodococcus species from Tunisia.

Abstract:

:The genus Rhodococcus contains several species with agricultural, biotechnological and ecological importance. Within this genus, many phyllosphere, rhizosphere and endosphere strains are plant growth promoting bacteria, whereas strains designated as R. fascians are plant pathogens. In this study, we isolated 47 Rhodococcus strains from a range of herbaceous and woody plant species. Phylogenetic analysis based on 16S rDNA, gyrB and alkB genes was used to compare our strains with type strains of Rhodococcus. For most of our strains, sequence similarity of the 16S rDNA, gyrB and alkB regions to type strains ranged from 98-100 %. Results of the concatenated gene sequence comparisons identified 18 strains of R. fascians and three strains of R. kroppenstedtii. The remaining strains were unclassified, and may represent novel species of Rhodococcus. Phylogenetic analysis based on gyrB sequences provided a more precise classification of our strains to species level than 16S rDNA sequences, whereas analysis of alkB sequences was unable to identify strains with orange-coloured colonies to species level.

authors

Dhaouadi S,Mougou AH,Wu CJ,Gleason ML,Rhouma A

doi

10.1099/ijsem.0.004521

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

6491-6507

issue

12

eissn

1466-5026

issn

1466-5034

journal_volume

70

pub_type

杂志文章
  • Taxonomy of haemolytic and/or proteolytic strains of the genus Acinetobacter with the proposal of Acinetobacter courvalinii sp. nov. (genomic species 14 sensu Bouvet & Jeanjean), Acinetobacter dispersus sp. nov. (genomic species 17), Acinetobacter modestu

    abstract::We aimed to define the taxonomic status of 40 haemolytic and/or proteolytic strains of the genus Acinetobacter which were previously classified into five putative species termed as genomic species 14BJ (n=9), genomic species 17 (n=9), taxon 18 (n=7), taxon 19 (n=6) and taxon 20 (n=9). The strains were recovered mostly...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000932

    authors: Nemec A,Radolfova-Krizova L,Maixnerova M,Vrestiakova E,Jezek P,Sedo O

    更新日期:2016-04-01 00:00:00

  • Ogataea haglerorum sp. nov., a novel member of the species complex, Ogataea (Hansenula) polymorpha.

    abstract::Three strains representing a novel species of the Ogataea clade were isolated by W. T. Starmer and H. J. Phaff from rotting tissue of Opuntia phaeacantha in Arizona, USA. Analyses of the sequences of the D1/D2 LSU rRNA gene, ITS1-5.8S-ITS2, and translation elongation factor-1α (EF-1 α) showed that this novel species b...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002012

    authors: Naumov GI,Naumova ES,Lee CF

    更新日期:2017-07-01 00:00:00

  • Paenibacillus darwinianus sp. nov., isolated from gamma-irradiated Antarctic soil.

    abstract::A novel bacterium, strain Br(T), was isolated from gamma-irradiated soils of the Britannia drift, Lake Wellman Region, Antarctica. This isolate was rod-shaped, endospore forming, Gram-stain-variable, catalase-positive, oxidase-negative and strictly aerobic. Cells possessed a monotrichous flagellum. Optimal growth was ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.056697-0

    authors: Dsouza M,Taylor MW,Ryan J,MacKenzie A,Lagutin K,Anderson RF,Turner SJ,Aislabie J

    更新日期:2014-04-01 00:00:00

  • Candida phangngensis sp. nov., an anamorphic yeast species in the Yarrowia clade, isolated from water in mangrove forests in Phang-Nga Province, Thailand.

    abstract::Two yeast strains (TM2-16 and PT1-17(T)) were isolated by membrane filtration from samples of estuarine water collected from two mangrove forests, in Khao Lumpee-Haad Thaimueang National Park and Mu Ko Ra-Ko Prathong National Park, Phang-Nga Province, Thailand. Analysis of the D1/D2 domain of the large-subunit rDNA se...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65506-0

    authors: Limtong S,Youngmanitchai W,Kawasaki H,Seki T

    更新日期:2008-02-01 00:00:00

  • Aquimarina megaterium sp. nov., isolated from seawater.

    abstract::A novel Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated XH134(T), was isolated from surface seawater of the South Pacific Gyre (45° 58' S 163° 11' W) during the Integrated Ocean Drilling Program Expedition 329. The major respiratory quinone of strain XH134(T)...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.055517-0

    authors: Yu T,Zhang Z,Fan X,Shi X,Zhang XH

    更新日期:2014-01-01 00:00:00

  • Classification of 'Sarraceniospora aurea' Furihata et al. 1989 as Actinocorallia aurea sp. nov.

    abstract::Phylogenetic analysis of 'Sarraceniospora aurea' NBRC 14752 and strain NBRC 15120, based on 16S rRNA gene sequences, revealed that these organisms are related to members of the genus Actinocorallia. These organisms contained glutamic acid, alanine and meso-diaminopimelic acid as cell-wall amino acids and the menaquino...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64008-0

    authors: Tamura T,Hatano K,Suzuki KI

    更新日期:2007-09-01 00:00:00

  • Prevotella aurantiaca sp. nov., isolated from the human oral cavity.

    abstract::Two anaerobic, pigmented, non-spore-forming, Gram-stain-negative, rod-shaped strains isolated from the human oral cavity, OMA31(T) and OMA130, were characterized by determining their phenotypic and biochemical features, cellular fatty acid profiles and phylogenetic positions based on 16S rRNA gene sequence analysis. 1...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.012831-0

    authors: Sakamoto M,Suzuki N,Okamoto M

    更新日期:2010-03-01 00:00:00

  • Rhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China.

    abstract::Two Gram-stain-negative, rod-shaped bacterial strains (C5T and C16), isolated from root nodules of Phaseolus vulgaris L. in Jiangxi Province, PR China, were characterized by using a polyphasic taxonomical approach. The phylogenetic analysis of the 16S rRNA gene and three concatenated housekeeping genes (recA-glnII-atp...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003430

    authors: Huo Y,Tong W,Wang J,Wang F,Bai W,Wang E,Shi P,Chen W,Wei G

    更新日期:2019-07-01 00:00:00

  • Polyphasic evidence for the reclassification of Rhodothermus obamensis Sako et al. 1996 as a member of the species Rhodothermus marinus Alfredsson et al. 1988.

    abstract::DNA-DNA reassociation studies, 16S rRNA gene sequence comparisons and fatty acid analysis were used to reassess the taxonomic status of the type strain of Rhodothermus obamensis and several strains of the genus Rhodothermus isolated from widely distributed shallow marine hot springs. The results show that the type str...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-4-1457

    authors: Silva Z,Horta C,da Costa MS,Chung AP,Rainey FA

    更新日期:2000-07-01 00:00:00

  • Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China.

    abstract::A Gram-positive, non-motile actinobacterium, designated strain YIM 70177(T), was isolated from a saline soil sample from Qinghai Province, north-west China. Phylogenetic analysis of this organism based on 16S rRNA gene sequences revealed 98.3% similarity to Isoptericola variabilis DSM 10177(T). Chemotaxonomic data det...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63641-0

    authors: Zhang YQ,Schumann P,Li WJ,Chen GZ,Tian XP,Stackebrandt E,Xu LH,Jiang CL

    更新日期:2005-09-01 00:00:00

  • Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004.

    abstract::Five novel Nocardiopsis strains isolated from hypersaline soils in China were subjected to a polyphasic analysis to determine their taxonomic position. All of the novel isolates could grow on agar plates at NaCl concentrations of up to 18 % (w/v), with optimum growth at 5-8 %. The DNA G+C contents of the novel strains...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64033-0

    authors: Li WJ,Kroppenstedt RM,Wang D,Tang SK,Lee JC,Park DJ,Kim CJ,Xu LH,Jiang CL

    更新日期:2006-05-01 00:00:00

  • Actinomadura harenae sp. nov., a novel actinomycete isolated from sea sand in Sanya.

    abstract::A novel actinomycete, designated strain NEAU-Ht49T, was isolated from sea sand sampled in Sanya and characterized by using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-Ht49T was most closely related to Actinomadura rhizosphaerae SDA37T (98.8 %), Actinomadura logoneensis NEAU-G17T ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003819

    authors: Hu J,Han C,Yu B,Zhao J,Guo X,Shen Y,Wang X,Xiang W

    更新日期:2020-02-01 00:00:00

  • Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters.

    abstract::A strictly anaerobic, thermophilic bacterium, designated strain AeB(T), was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the Great Artesian Basin, Australia. Cells of strain AeB(T) were slightly curved rods (2.5-6.0x1.0 mum...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.014670-0

    authors: Ogg CD,Patel BKC

    更新日期:2010-06-01 00:00:00

  • Roseithermus sacchariphilus gen. nov., sp. nov. and proposal of Salisaetaceae fam. nov., representing new family in the order Rhodothermales.

    abstract::A novel bacterium with cells that were pinkish-cream-coloured, aerobic, rod-shaped, 0.62-1.00 µm wide and 2.3-3.3 µm long, designated as strain MEBiC09517T, was isolated from Buksung-Po, a small port in Incheon, Republic of Korea. Strain MEBiC09517T had low 16S rRNA gene sequence similarity to validly reported strains...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003293

    authors: Park MJ,Oh JH,Yang SH,Kwon KK

    更新日期:2019-04-01 00:00:00

  • Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps).

    abstract::The fluorescent amplified fragment length polymorphism (FAFLP) groups A5 (21 isolates), A8 (6 isolates) and A23 (3 isolates) distinguished in an earlier paper (Thompson et al., Syst Appl Microbiol 24, 520-538, 2001) were examined in more depth. These three groups were phylogenetically related to Vibrio tubiashii, but ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02447-0

    authors: Thompson FL,Li Y,Gomez-Gil B,Thompson CC,Hoste B,Vandemeulebroecke K,Rupp GS,Pereira A,De Bem MM,Sorgeloos P,Swings J

    更新日期:2003-01-01 00:00:00

  • Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides x nigra DN34).

    abstract::A pink-pigmented, aerobic, facultatively methylotrophic bacterium, strain BJ001T, was isolated from internal poplar tissues (Populus deltoidesxnigra DN34) and identified as a member of the genus Methylobacterium. Phylogenetic analyses showed that strain BJ001T is related to Methylobacterium thiocyanatum, Methylobacter...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02796-0

    authors: Van Aken B,Peres CM,Doty SL,Yoon JM,Schnoor JL

    更新日期:2004-07-01 00:00:00

  • Rothia endophytica sp. nov., an actinobacterium isolated from Dysophylla stellata (Lour.) Benth.

    abstract::A novel endophytic actinobacterium, designated strain YIM 67072(T), was isolated from healthy roots of Dysophylla stellata (Lour.) Benth. Cells of this aerobic, cream-yellow-coloured strain occurred singly, in pairs or in tetrads, were Gram-stain-positive and ovoid- to spherical-shaped. Strain YIM 67072(T) grew at 4-4...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.052522-0

    authors: Xiong ZJ,Zhang JL,Zhang DF,Zhou ZL,Liu MJ,Zhu WY,Zhao LX,Xu LH,Li WJ

    更新日期:2013-11-01 00:00:00

  • Fluviicola taffensis gen. nov., sp. nov., a novel freshwater bacterium of the family Cryomorphaceae in the phylum 'Bacteroidetes'.

    abstract::A novel, strictly aerobic, Gram-negative, yellow-orange-pigmented, motile, catalase-positive, oxidase-negative bacterium, RW262(T), was isolated from water of the River Taff, Cardiff, UK, during January 2000. Phylogenetic analysis of the 16S rRNA gene indicated that strain RW262(T) was a member of the family Cryomorph...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63736-0

    authors: O'Sullivan LA,Rinna J,Humphreys G,Weightman AJ,Fry JC

    更新日期:2005-09-01 00:00:00

  • Postechiella marina gen. nov., sp. nov., isolated from seawater.

    abstract::A Gram-staining-negative, strictly aerobic, non-motile, yellow-pigmented bacterium, designated strain M091(T), was isolated from seawater at Damupo beach in Pohang, Republic of Korea, and investigated using a polyphasic taxonomic approach. The novel strain grew optimally at 25 °C, pH 7.0-8.0, and in the presence of 3%...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.031302-0

    authors: Lee DH,Cho SJ,Kim SM,Lee SB

    更新日期:2012-07-01 00:00:00

  • Thermocatellispora soli sp. nov., isolated from hot spring soil.

    abstract::An aerobic, spore-forming, actinomycete, designated strain CHM3-46T, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand. The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora. Strain CHM3-46T produced short, straight chains of warty spores on aeria...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003115

    authors: Duangupama T,Pittayakhajonwut P,Thawai C

    更新日期:2019-01-01 00:00:00

  • Kribbella catacumbae sp. nov. and Kribbella sancticallisti sp. nov., isolated from whitish-grey patinas in the catacombs of St Callistus in Rome, Italy.

    abstract::Several nocardioform actinomycetes were isolated from tufaceous surfaces with whitish-grey patinas in the catacombs of St Callistus in Rome, Italy. The morphology of the isolates and their chemotaxonomic characteristics such as LL-diaminopimelic acid in the cell-wall peptidoglycan, the major menaquinone of MK-9(H(4)),...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65613-0

    authors: Urzì C,De Leo F,Schumann P

    更新日期:2008-09-01 00:00:00

  • Aureimonas ferruginea sp. nov. and Aureimonas rubiginis sp. nov., two siderophore-producing bacteria isolated from rusty iron plates.

    abstract::Two novel siderophore-producing strains designated CC-CFT023(T) and CC-CFT034(T), isolated from a rusty iron plate, are characterized in this study. Cells of both strains were catalase- and oxidase-positive. Strain CC-CFT023(T) showed a high pairwise 16S rRNA gene sequence similarity to Aureimonas altamirensis LMG 233...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.047431-0

    authors: Lin SY,Hameed A,Liu YC,Hsu YH,Lai WA,Shen FT,Young LS,Tsai CF,Young CC

    更新日期:2013-07-01 00:00:00

  • Nocardioides vastitatis sp. nov., isolated from Taklamakan desert soil.

    abstract::A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach. Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003718

    authors: Liu SW,Xue CM,Li FN,Sun CH

    更新日期:2020-01-01 00:00:00

  • Alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep South African gold mine.

    abstract::A novel extreme alkaliphile was isolated from a mine water containment dam at 3.2 km below land surface in an ultra-deep gold mine near Carletonville, South Africa. The cells of this bacterium were straight to slightly curved rods, motile by flagella and formed endospores. Growth was observed over the temperature rang...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-51-4-1245

    authors: Takai K,Moser DP,Onstott TC,Spoelstra N,Pfiffner SM,Dohnalkova A,Fredrickson JK

    更新日期:2001-07-01 00:00:00

  • Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass.

    abstract::Chironomids (Diptera; Chironomidae) are the most abundant insects in freshwater aquatic habitats. Females of the genus Chironomus lay egg masses containing hundreds of eggs embedded in a gelatinous matrix. A bacterial strain, designated T3944D(T), was isolated from a chironomid egg mass sampled from a waste-stabilizat...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64502-0

    authors: Raats D,Halpern M

    更新日期:2007-02-01 00:00:00

  • Deinococcus metallilatus sp. nov. and Deinococcus carri sp. nov., isolated from a car air-conditioning system.

    abstract::Two bacterial strains, designated MA1002(T) and MA1003(T), were isolated from the air-conditioning system of a car. Cells of both strains were Gram-reaction-positive, non-motile, non-spore-forming coccoids, catalase- and oxidase-positive and UV-radiation resistant. The major fatty acids of strain MA1002(T) were iso-C1...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000396

    authors: Kim DU,Lee H,Lee JH,Ahn JH,Lim S,Jeong S,Park SY,Seong CN,Ka JO

    更新日期:2015-09-01 00:00:00

  • Gemmobacter tilapiae sp. nov., a poly-β-hydroxybutyrate-accumulating bacterium isolated from a freshwater pond.

    abstract::A novel bacterium, designated strain Ruye-53(T), was isolated from a freshwater pond used to rear tilapiine cichlid fish in Taiwan. The taxonomic position of the novel strain was determined using a polyphasic approach. Strain Ruye-53(T) was Gram-reaction-negative, aerobic, cream-white coloured, rod-shaped, non-motile ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.044735-0

    authors: Sheu SY,Sheu DS,Sheu FS,Chen WM

    更新日期:2013-04-01 00:00:00

  • Methylotenera oryzisoli sp. nov., a lanthanide-dependent methylotrophic bacteria isolated from rice field soil.

    abstract::A new lanthanide (Ln3+)-dependent methanol-utilizing bacterial strain, La3113T, was isolated from rice field soil and its taxonomic position was investigated using polyphasic approaches. The strain was aerobic, Gram-stain-negative, strongly motile, catalase-positive and cytochrome oxidase-positive. It could neither ca...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004098

    authors: Lv H,Sahin N,Tani A

    更新日期:2020-04-01 00:00:00

  • Paenibacillus sputi sp. nov., isolated from the sputum of a patient with pulmonary disease.

    abstract::Strain KIT 00200-70066-1(T) was isolated from the sputum of a patient with pulmonary disease. Cells of the strain were Gram-variable, facultatively anaerobic, motile, spore-forming rods and formed colourless to white colonies on tryptic soy agar at 30 °C and pH 7. The pathogenicity of the strain is not k...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.017137-0

    authors: Kim KK,Lee KC,Yu H,Ryoo S,Park Y,Lee JS

    更新日期:2010-10-01 00:00:00

  • Ulvibacter antarcticus sp. nov., isolated from Antarctic coastal seawater.

    abstract::A seawater bacterium, designated IMCC3101(T), was isolated from Antarctic coastal seawater. The strain was Gram-negative, chemoheterotrophic, obligately aerobic, pigmented dark yellow (flexirubin-type pigments) and devoid of gliding and flagellar motility. On the basis of 16S rRNA gene sequence comparisons, the most c...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65265-0

    authors: Choi TH,Lee HK,Lee K,Cho JC

    更新日期:2007-12-01 00:00:00