Abstract：
:Previous studies have demonstrated that various microRNAs (miRNAs or miRs) are abnormally expressed in osteosarcoma (OS) and serve roles in its malignant development. An in-depth understanding of the specific roles of dysregulated miRNAs in OS may be important for cancer research and the identification of novel therapeutic targets. In the current study, reverse transcription-quantitative PCR was performed to determine miR-652 expression in OS tissues and cell lines. Cell Counting Kit-8 and Transwell invasion assays were used for assessing the effect of miR-652 on the proliferation and invasion of OS cells. Herein, miR-652 expression was assessed in OS and the effects and molecular mechanisms of miR-652 in OS cells were examined. The results revealed that miR-652 expression was significantly upregulated in OS tissues and cell lines compared with adjacent normal tissues and a normal human osteoblast cell line. Furthermore, miR-652 downregulation inhibited the proliferation and invasion of OS cells. miR-652 was also demonstrated to directly interact with the 3'-untranslated region of kruppel-like factor 9 (KLF9) and miR-652 negatively regulated KLF9 expression in OS cells. miR-652 and KLF9 mRNA levels were also revealed to be inversely correlated in OS tissues. Treatment with KLF9 small interfering RNA abolished the suppression of OS proliferation and invasion induced by miR-652 downregulation. miR-652 may serve an oncogenic role in OS cells by targeting KLF9 directly. The results also indicated that miR-652 may be an effective novel therapeutic target for the treatment of patients with OS.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Jin Y,Yang L,Li Xdoi
10.3892/etm.2020.9037subject
Has Abstractpub_date
2020-10-01 00:00:00pages
2953-2960issue
4eissn
1792-0981issn
1792-1015pii
ETM-0-0-9037journal_volume
20pub_type
杂志文章abstract：:Pancreatic necrosectomy (PN) following percutaneous catheter drainage (PCD) is an effective method of treating patients with necrotizing pancreatitis, however, the predictive factors for PN after PCD have not yet been identified. A total of 74 patients with suspected infected necrotizing pancreatitis (INP) and peripan...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
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journal_title：Experimental and therapeutic medicine
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更新日期：2017-10-01 00:00:00
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journal_title：Experimental and therapeutic medicine
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更新日期：2017-06-01 00:00:00
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journal_title：Experimental and therapeutic medicine
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更新日期：2012-12-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.7121
更新日期：2019-03-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
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更新日期：2020-04-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5494
更新日期：2018-01-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6648
更新日期：2018-10-01 00:00:00
abstract：:[This corrects the article DOI: 10.3892/etm.2017.4943.]. ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章,已发布勘误
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更新日期：2018-10-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9188
更新日期：2020-11-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6193
更新日期：2018-07-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
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更新日期：2019-02-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
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更新日期：2019-06-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
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更新日期：2017-06-01 00:00:00
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更新日期：2021-01-01 00:00:00
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journal_title：Experimental and therapeutic medicine
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更新日期：2020-09-01 00:00:00
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更新日期：2018-04-01 00:00:00
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journal_title：Experimental and therapeutic medicine
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更新日期：2017-12-01 00:00:00
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journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
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更新日期：2014-05-01 00:00:00
abstract：:Professor Alan Michael Weindling, Emeritus Professor of Perinatal Medicine at the University of Liverpool in the UK, is one of the pioneers of modern neonatal care worldwide with an enormous contribution to the care of premature babies in the city of Liverpool. During the last 3 decades, his contributions focused on t...
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更新日期：2018-09-01 00:00:00
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更新日期：2017-08-01 00:00:00
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更新日期：2019-12-01 00:00:00
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