当前位置： SCI文献检索 > mBio期刊下所有文献 > Host-Microbe Interactions in the Chemosynthetic Riftia pachyptila Symbiosis.

Host-Microbe Interactions in the Chemosynthetic Riftia pachyptila Symbiosis.

Abstract：

:The deep-sea tubeworm Riftia pachyptila lacks a digestive system but completely relies on bacterial endosymbionts for nutrition. Although the symbiont has been studied in detail on the molecular level, such analyses were unavailable for the animal host, because sequence information was lacking. To identify host-symbiont interaction mechanisms, we therefore sequenced the Riftia transcriptome, which served as a basis for comparative metaproteomic analyses of symbiont-containing versus symbiont-free tissues, both under energy-rich and energy-limited conditions. Our results suggest that metabolic interactions include nutrient allocation from symbiont to host by symbiont digestion and substrate transfer to the symbiont by abundant host proteins. We furthermore propose that Riftia maintains its symbiont by protecting the bacteria from oxidative damage while also exerting symbiont population control. Eukaryote-like symbiont proteins might facilitate intracellular symbiont persistence. Energy limitation apparently leads to reduced symbiont biomass and increased symbiont digestion. Our study provides unprecedented insights into host-microbe interactions that shape this highly efficient symbiosis.IMPORTANCE All animals are associated with microorganisms; hence, host-microbe interactions are of fundamental importance for life on earth. However, we know little about the molecular basis of these interactions. Therefore, we studied the deep-sea Riftia pachyptila symbiosis, a model association in which the tubeworm host is associated with only one phylotype of endosymbiotic bacteria and completely depends on this sulfur-oxidizing symbiont for nutrition. Using a metaproteomics approach, we identified both metabolic interaction processes, such as substrate transfer between the two partners, and interactions that serve to maintain the symbiotic balance, e.g., host efforts to control the symbiont population or symbiont strategies to modulate these host efforts. We suggest that these interactions are essential principles of mutualistic animal-microbe associations.

journal_name

mBio

journal_title

mBio

authors

Hinzke T,Kleiner M,Breusing C,Felbeck H,Häsler R,Sievert SM,Schlüter R,Rosenstiel P,Reusch TBH,Schweder T,Markert S

doi

10.1128/mBio.02243-19

subject

Has Abstract

pub_date

2019-12-17 00:00:00

issue

issn

2150-7511

pii

mBio.02243-19

journal_volume

pub_type

杂志文章

在线工具

Host-Microbe Interactions in the Chemosynthetic Riftia pachyptila Symbiosis.