Abstract:
Purpose:Sjögren syndrome (SS) is an autoimmune disease characterized by the inflammatory destruction of salivary and lacrimal glands (LG). Chloroquine (CQ) was known as an immunomodulatory drug and in the inhibition of autophagy. The purpose of the study is to investigate the effect of CQ on the development of dry eye in NOD-LtJ mice. Methods:NOD-LtJ mice were observed, during which the occurrence of dry eye was confirmed by tear secretion, corneal staining, and the infiltration of foci into the LG from 13-week-old mice. Intraperitoneal (IP) administration of CQ was performed in 13-week-old mice for 4 weeks and maintained untreated for another 4 weeks. Additionally, CQ was injected IP in 19-week-old mice for 2 weeks from when the disease was fully developed. Results:Interestingly, the expression of autophagy marker ATG5 and LC3B-II was observed in the LG from week 5. When CQ had been administered for 4 weeks from week 13 and then maintained untreated for 4 weeks, tear secretion, corneal staining score, foci formation in the LG, conjunctival goblet cells and proinflammatory cytokine expressions were significantly better than untreated mice. The infiltration of immune cells and the expression of autophagy markers in LG were decreased in the CQ group. These indices improved significantly as well when the 19-week-old mice with severe clinical phenotypes had been treated with CQ for 2 weeks. Conclusions:This study demonstrated that autophagy was induced in the early stages of the SS model and that CQ treatment in the early stages could inhibit disease progression.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Lee HJ,Shin S,Yoon SG,Cheon EJ,Chung SHdoi
10.1167/iovs.19-27469subject
Has Abstractpub_date
2019-09-03 00:00:00pages
3708-3716issue
12eissn
0146-0404issn
1552-5783pii
2749570journal_volume
60pub_type
杂志文章abstract::The corneas of 12 eye donors with maturity-onset diabetes were obtained, and the corneal epithelial basement membranes were studied by transmission electron microscopy. Similar tissue was obtained from 12 nondiabetic eye donors who were matched for age (within 2 years) and race. The mean thickness of the corneal epith...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1981-04-01 00:00:00
abstract:PURPOSE:To develop structural markers of early glaucomatous optic nerve damage with confocal scanning laser ophthalmoscopy. METHODS:Custom software was developed to analyze the images of 53 patients with open-angle glaucoma and 43 healthy subjects (matched for age, race, and refractive error), with images acquired wit...
journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1998-11-01 00:00:00
abstract:Purpose:To determine the long-term safety and efficacy of WST-D/near-infrared (NIR) corneal stiffening. Methods:One eye of 23 New Zealand White rabbits was de-epithelialized mechanically followed by topical application of 2.5 mg/mL WST11, combined with dextran-500 (WST-D) for 20 minutes. Subsequently, samples were irr...
journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.17-22161
更新日期:2017-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1979-12-01 00:00:00
abstract:Purpose:Dense deposit disease (DDD) is caused by dysregulation of the alternative pathway of the complement cascade and characterized by electron-dense deposits in the kidney glomerular basement membrane (GBM) and drusen in Bruch's membrane (BrM). Complement factor H (fH) and factor properdin (fP) regulate complement a...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-24133
更新日期:2018-07-02 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1981-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
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pub_type: 临床试验,杂志文章
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journal_title:Investigative ophthalmology & visual science
pub_type: 临床试验,杂志文章
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1977-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1997-09-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2011-01-21 00:00:00
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1982-11-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
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更新日期:2011-04-08 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
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