Abstract:
:Despite intensive studies of the Trichophyton mentagrophytes species complex, its taxonomy still causes confusion. In this study, more than 70 dermatophytes were analyzed based on nuc rDNA ITS1-5.8S-ITS2 (ITS), D1-D2 domains of nuc 28S rDNA (D1D2), and β-tubulin gene (TUBB) sequences to clarify phylogenetic relationships in the complex. This demonstrated that strains of the complex were divided into three major lineages with high statistical support: (i) T. benhamiae and related species; (ii) T. simii and two related species, T. quinckeanum and T. schoenleinii; and (iii) T. mentagrophytes, T. interdigitale, and related species. The major lineages could be further divided into 18 phylogroups, representing either individual species or phylogenetically distinct groups within species. Among strains of T. benhamiae, African isolates American Type Culture Collection (ATCC) 28064 and 28065 formed a phylogenetically distinct phylogroup from their type strain and were considered a distinct species. Strains of T. mentagrophytes were divided into at least four phylogroups based on combined sequence analysis, but some phylogroups showed closer relationships to T. interdigitale, T. equinum, and T. tonsurans when compared by individual genes. This indicates that identifying those species with one gene could lead to incorrect results. For rapid identification of those dermatophytes, each phylogroup was tested by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry using a database with customized reference spectra of each phylogroup. This system was able to identify all the tested strains to species level with higher than 91% accuracy, except for strains of T. interdigitale. The three phylogroups of T. benhamiae were well distinguished from one another with high identification accuracy, whereas phylogroups of T. mentagrophytes were often cross-identified to one another or to T. interdigitale. Further research should improve identification accuracy for some species, but the results suggested that MALDI-TOF MS could be a rapid and efficient identification tool for closely related dermatophytes in the T. mentagrophytes species complex.
journal_name
Mycologiajournal_title
Mycologiaauthors
Suh SO,Grosso KM,Carrion MEdoi
10.1080/00275514.2018.1443653subject
Has Abstractpub_date
2018-01-01 00:00:00pages
118-130issue
1eissn
0027-5514issn
1557-2536journal_volume
110pub_type
杂志文章相关文献
MYCOLOGIA文献大全abstract::Following the abandonment of dual nomenclature and the implementation of single-name nomenclature for pleomorphic fungi, Coryneum was considered to have priority over Pseudovalsa and was recommended for use. Currently, Coryneum is the only genus in the family Coryneaceae (Diaporthales). However, DNA sequence data are ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2018.1516969
更新日期:2018-11-01 00:00:00
abstract::Molecular and ultrastructural investigations are revealing unrealized diversity among chytrids as the taxonomic revision of order Chytridiales (Chytridiomycota) progresses. During a biodiversity survey of soil-inhabiting chytrids in Australia an undescribed chytrid was isolated from a soil sample collected in a cool t...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/08-194
更新日期:2009-09-01 00:00:00
abstract::Six penzigioid Xylaria species that are characterized by small, discoid to pulvinate, soft stromata are included in this study. Xylaria albocinctoides, X. bicampaniformis and X. lechatii are described as new; Nummularia albocincta, Hypoxylon carabayense and H. discolor are moved to the genus Xylaria to form new combin...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/11-313
更新日期:2012-05-01 00:00:00
abstract::This contribution to the genus Cantharellus in North America deals with the smaller, reddish pink species from the Gulf of Mexico states and eastern United States. C. texensis sp. nov. is presented as a new southern lookalike of C. cinnabarinus. The morphological species concepts are supported by newly generated molec...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/10-261
更新日期:2011-09-01 00:00:00
abstract::Soil fungal communities are responsible for carbon and nitrogen (N) cycling. The high complexity of the soil fungal community and the high proportion of taxonomically unidentifiable sequences confound ecological interpretations in field studies because physiological information is lacking for many organisms known only...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/16-086
更新日期:2016-11-01 00:00:00
abstract::The large genus Leucoagaricus (Basidiomycota) is poorly studied in Pakistan, where the northern parts of the country are considered hot spots for biodiversity. Based on morphological and molecular data, five new species are described: Leucoagaricus badius, L. lahorensiformis, L. pakistaniensis, L. sultanii, and L. umb...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2018.1439651
更新日期:2018-03-01 00:00:00
abstract::The genus Protocrea is redefined, based on holotype and fresh specimens of its type species P. farinosa, using morphology of teleomorph and anamorph and phylogenetic analyses of rpb2 sequences. Data based on currently available specimens suggest the existence of three well defined and three still unnamed species. Apar...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/08-101
更新日期:2008-11-01 00:00:00
abstract::Dictyostelids are a monophyletic group of sorocarp-forming social amoebae in the major eukaryotic division Amoebozoa. Members of this taxon, which is made up of almost 200 described species, are common in terrestrial soils globally. Still, the alpha diversity is not well known in many areas, and new species are freque...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2020.1802641
更新日期:2020-09-01 00:00:00
abstract::The conidiation of the entomopathogenic fungus Beauveria bassiana (Hyphomycete) is a complex process that involves the stage- and cell-type-specific expression of hundreds of genes. The suppression subtractive hybridization method was used to target genes involved in conidiation. Seventeen genes were cloned that poten...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.100.1.20
更新日期:2008-01-01 00:00:00
abstract::Periconia variicolor was isolated from water of an evaporation pond used for salt production on the southwestern coast of Puerto Rico. On the basis of cultural and morphological characteristics and ITS sequence it is describe as a new species of Periconia. ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.99.3.482
更新日期:2007-05-01 00:00:00
abstract::Pilobolus, a widely distributed coprophilous fungus, grows on herbivore dung. Species of Pilobolus traditionally are described with imprecise morphological characteristics potentially leading to misidentification. In this study we used PCR analysis of taxonomically informative sequences to provide more consistent spec...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/11-107
更新日期:2011-11-01 00:00:00
abstract::Two new species of Phylloporus from Bangladesh forests dominated by Shorea robusta are described and illustrated, based on morphological and molecular phylogenetic evidence. Phylloporus attenuatus is characterized by its wine-red to brownish-red pilei, decurrent lamellae that stain blue, downwards tapering stipe, a tr...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2017.1312196
更新日期:2017-01-01 00:00:00
abstract::Mitochondrial DNA restriction fragment length polymorphisms (RFLPs) are described for 64 isolates, representing 11 species of Aspergillus section Flavi. Mitochondrial DNA haplotypes were identified following digestion of total cellular DNA with the restriction enzymes HaeIII, AseI, or DraI. In general, isolates of the...
journal_title:Mycologia
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::Nivicolous myxomycetes are a group of amoebozoan protists dependent on long-lasting snow cover worldwide. Recent fine-scale analysis of species diversity from the austral Andes revealed high intraspecific variability of most taxa, suggesting independent evolutionary processes and significant differences in species com...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2020.1759978
更新日期:2020-07-01 00:00:00
abstract::Cool-season grasses (Poaceae subfamily Poöideae) are an important forage component for livestock in western China, and many have seed-transmitted symbionts of the genus Epichloë, fungal endophytes that are broadly distributed geographically and in many tribes of the Poöideae. Epichloë strains can produce any of severa...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2018.1426941
更新日期:2017-01-01 00:00:00
abstract::Several cases of speciation by hybridization have been reported in fungi, mostly involving recent hybridization between closely related species. In the basidiomycete genus Microbotryum by contrast some species were suspected to have arisen by hybridization between moderately distant species. In particular two species,...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/09-192
更新日期:2010-05-01 00:00:00
abstract::We investigated the influence of tilling, N fertilization and crop stage on arbuscular mycorrhizae (AM) fungal species diversity in a wheat monoculture in the Pampa region of Argentina. Glomalean spores were isolated by wet sieving and decanting from conventionally tilled and nontilled soils cropped with wheat with or...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.98.1.16
更新日期:2006-01-01 00:00:00
abstract::Many rust fungi (Uredinales) that infect rhododendrons are difficult to identify because of similar spore size and overall morphology. As part of a morphological study of rusts in the genus Chrysomyxa, herbarium specimens of Asian rhododendron rusts were examined by light and scanning electron microscopy. They were co...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.97.2.534
更新日期:2005-03-01 00:00:00
abstract::Binderoboletus segoi gen. and sp. nov., Guyanaporus albipodus gen. and sp. nov. and Singerocomus rubriflavus gen. and sp. nov. (Boletaceae, Boletales, Basidiomycota) are described from the Pakaraima Mountains and adjacent lowlands of Guyana. Xerocomus inundabilis, originally described from the central Brazilian Amazon...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/15-075
更新日期:2016-01-01 00:00:00
abstract::Elaphomyces compleximurus sp. nov. and E. digitatus sp. nov. are described from the Pakaraima Mountains of Guyana. Macromorphological, micromorphological, habitat and DNA sequence data are provided for each new species. This is the first report of Elaphomyces ascomata associated with ectomycorrhizal members of the Fab...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/12-061
更新日期:2012-09-01 00:00:00
abstract::The Carboniferous microfungus Protoascon missouriensis has been interpreted variously as an ascomycete, chytridiomycete, zygomycete and oomycete. We offer a more complete interpretation based on a re-examination of the type material that suggests the fossil represents an (a)zygosporangium-suspensor complex of a zygomy...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.97.3.725
更新日期:2005-05-01 00:00:00
abstract::Xylaria hypoxylon, the type species of Xylaria (Xylariaceae, Sordariomycetes), was first described by Linnaeus as Clavaria hypoxylon from Sweden. Saccardo and other mycologists assumed a cosmopolitan distribution for this species. However, contradictory reports in the literature on its morphoanatomical characters and ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/08-108
更新日期:2009-03-01 00:00:00
abstract::Two interesting fungi belonging to the genus Cordana have been isolated recently in Spain from plant debris. Both are proposed here as new species, described and illustrated. Cordana mercadiana sp. nov. produces 0-1-septate conidia, with a prominent basal scar. Cordana verruculosa sp. nov. differs from the other speci...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/13-122
更新日期:2014-07-01 00:00:00
abstract::Early Holocene bison mandibles (Bison antiquus) from Nebraska, ca. 8500 y ago, were examined with a variety of modern histotechnological procedures and staining techniques. A pathological, anatomical diagnosis of moderately severe, locally extensive, mandibular osteomyelitis with intralesional spherules morphologicall...
journal_title:Mycologia
pub_type: 历史文章,杂志文章
doi:10.3852/mycologia.98.5.669
更新日期:2006-09-01 00:00:00
abstract::Three new bitunicate ascomycetes belonging to the genus Acanthostigma are described from terrestrial decomposing wood collected from Great Smoky Mountains National Park, USA. Phylogenetic analyses of the nuclear ribosomal 28S large subunit and internal transcribed spacer region placed all three species in the Tubeufia...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/09-051
更新日期:2010-05-01 00:00:00
abstract::Three species of Cercophora were found during a survey of the biodiversity of microfungi in northwest Argentina. Cercophora argentina possesses a unique combination of morphological characters and is described as a new species, while C. costaricensis and C. solaris are reported as new records for Argentina. Other spec...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/11-005
更新日期:2011-11-01 00:00:00
abstract::The genus Agaricus is known for its medicinal and edible species but also includes toxic species that belong to section Xanthodermatei. Previous phylogenetic reconstruction for temperate species, based on sequence data of nuc rRNA gene (rDNA) internal transcribed spacers (ITS), has revealed two major groups in this se...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/14-076
更新日期:2014-11-01 00:00:00
abstract::Three species of dictyostelid cellular slime molds were isolated from forest soil, meadow soil and leaf litter collected from Yalta, Crimea, Ukraine. Dictyostelium globisporum is new to science; D. crassicaule and D. sphaerocephalum are new records from Ukraine. Descriptions and illustrations are given based on the Uk...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/10-253
更新日期:2011-05-01 00:00:00
abstract::We evaluated sporangium and zoospore production by three isolates of Phytophthora ramorum on Rhododendron 'Cunningham's White' leaves under light and dark conditions at both variable and constant (14 C) temperature. P. ramorum-infected leaves were detached and placed in funnels inside of a 62-L plastic storage contain...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2020.1728472
更新日期:2020-05-01 00:00:00
abstract::Morphological, phylogenetic (sequencing of the ribosomal ITS region) and, if applicable, biological (pairings of single-spore testers) species recognition have been used to resolve relationships among 69 collections belonging to the Hymenochaetales genera Phellinus s.str. and Fomitiporia. The isolates originate from a...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/15572536.2005.11832927
更新日期:2004-07-01 00:00:00