Direct Contact Test for Evaluating Bacterial Growth on Machined and Rough Surface Implants: An In Vitro Study.

Abstract:

OBJECTIVE:To use a modified direct contact test (DCT) to evaluate the bacterial growth on 3 commercially available implants. MATERIALS AND METHODS:Streptococcus salivarius growth was tested on 1 machined surface and 2 commercial rough surface implants. Seven implants from each group were fixed to the cover of a 96-well microtiter plate. Bacterial suspension was placed on each implant, and the plate was incubated at 37°C for 1 hour to allow the suspension fluid to evaporate. Fresh medium was added to 7 corresponding wells in the microtiter plate, and the cover holding the implants was placed on the plate to enable immersion of the implant. Noncontaminated implant samples served as control. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 hours. RESULTS:There was a significant S. salivarius growth in all groups of test implants compared with the control. There was no significant difference between bacterial growth kinetics on treated and nontreated implant surfaces (P = 0.241). CONCLUSION:Using the DCT, we found no difference in bacterial growth between machined and rough surface implants.

journal_name

Implant Dent

journal_title

Implant dentistry

authors

Slutzkey S,Moses O,Tal H,Meirowitz A,Matalon S

doi

10.1097/ID.0000000000000697

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

899-903

issue

6

eissn

1056-6163

issn

1538-2982

journal_volume

26

pub_type

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