Abstract:
:The drug-induced accumulation of phospholipids in lysosomes of various tissues is predominantly observed in regular repeat dose studies, often after prolonged exposure, and further investigated in mechanistic studies prior to candidate nomination. The finding can cause delays in the discovery process inflicting high costs to the affected projects. This article presents an in vitro imaging-based method for early detection of phospholipidosis liability and a hybrid approach for early detection and risk mitigation of phospolipidosis utilizing the in vitro readout with in silico model prediction. A set of reference compounds with phospolipidosis annotation was used as an external validation set yielding accuracies between 77.6% and 85.3% for various in vitro and in silico models, respectively. By means of a small set of chemically diverse known drugs with in vivo phospholipidosis annotation, the advantages of combining different prediction methods to reach an overall improved phospholipidosis prediction will be discussed.
journal_name
Mol Pharmjournal_title
Molecular pharmaceuticsauthors
Fusani L,Brown M,Chen H,Ahlberg E,Noeske Tdoi
10.1021/acs.molpharmaceut.7b00388subject
Has Abstractpub_date
2017-12-04 00:00:00pages
4346-4352issue
12eissn
1543-8384issn
1543-8392journal_volume
14pub_type
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