当前位置： SCI文献检索 > MOLECULAR PHYLOGENETICS AND EVOLUTION期刊下所有文献 > Resolution of the enigmatic phylogenetic relationship of the critically endangered Western Swamp Tortoise Pseudemydura umbrina (Pleurodira: Chelidae) using a complete mitochondrial genome.

Resolution of the enigmatic phylogenetic relationship of the critically endangered Western Swamp Tortoise Pseudemydura umbrina (Pleurodira: Chelidae) using a complete mitochondrial genome.

Abstract：

:Pseudemydura umbrina is one of the most endangered turtle species in the world, and the imperative for its conservation is its distinctive morphology and relict status among the Chelidae. We use Illumina sequencing to obtain the complete mitogenome for resolving its uncertain phylogenetic position. A novel nuclear paralogue confounded the assembly, and resolution of the authentic mitogenome required further Sanger sequencing. The P. umbrina mitogenome is 16,414bp comprising 37 genes organized in a conserved pattern for other vertebrates. The nuclear paralogue is 547bp, 97.8% identity to the corresponding mitochondrial sequence. Particular features of the mitogenome include an nd3 174+1A frameshift, loss of DHC loop in tRNASer (AGN), and a light-strand replication initiation site in Wancy region that extends into an adjacent tRNA gene. Phylogenetic analysis showed that P. umbrina is the monotypic sister lineage to the remaining Australasian Chelidae, a lineage probably dating back to the Cretaceous.

journal_name

Mol Phylogenet Evol

journal_title

Molecular phylogenetics and evolution

authors

Zhang X,Unmack PJ,Kuchling G,Wang Y,Georges A

doi

10.1016/j.ympev.2017.07.019

subject

Has Abstract

pub_date

2017-10-01 00:00:00

pages

58-61

eissn

1055-7903

issn

1095-9513

pii

S1055-7903(17)30201-4

journal_volume

115

pub_type

杂志文章

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