Abstract:
BACKGROUND/AIM:In this study, we aimed to investigate the additive effect of mesenchymal stem cells (MSC) and defibrotide (DFT) in a rat model of femoral arterial thrombosis. METHODS:Thirty Sprague Dawley rats were included. An arterial thrombosis model by ferric chloride (FeCl3) was developed in the left femoral artery. The rats were equally assigned to 5 groups: Group 1-Sham-operated (without arterial injury); Group 2-Phosphate buffered saline (PBS) injected; Group 3-MSC; Group 4-DFT; Group 5-MSC + DFT. All had two intraperitoneal injections of 0.5 ml: the 1st injection was 4 h after the procedure and the 2nd one 48 h after the 1st injection. The rats were sacrificed 7 days after the 2nd injection. RESULTS:Although the use of human bone marrow-derived (hBM) hBM-MSC or DFT alone enabled partial resolution of the thrombus, combining them resulted in near-complete resolution. Neovascularization was two-fold better in hBM-MSC + DFT treated rats (11.6 ± 2.4 channels) compared with the hBM-MSC (3.8 ± 2.7 channels) and DFT groups (5.5 ± 1.8 channels) (P < 0.0001 and P= 0.002, respectively). CONCLUSION:The combined use of hBM-MSC and DFT in a rat model of arterial thrombosis showed additive effect resulting in near-complete resolution of the thrombus. INTRODUCCIÓN:El objetivo de este estudio consistió en investigar el efecto aditivo de las células madre mesenquimales (MSC, por sus siglas en inglés) y del defibrótido (DFT) en un modelo de trombosis arterial femoral en ratas. MÉTODOS:Se incluyeron 30 ratas Sprague Dawley. Se desarrolló un modelo de trombosis arterial mediante cloruro de hierro (FeCl3) en la arteria femoral izquierda. Las ratas se asignaron equitativamente en cinco grupos: grupo 1, intervención quirúrgica simulada (sin lesión arterial); grupo 2, inyección de solución salina tamponada con fosfato (PBS); grupo 3, MSC; grupo 4, DFT; grupo 5, MSC + DFT. Todas las ratas recibieron dos inyecciones intraperitoneales de 0,5 ml: la primera se administró 4 horas después del procedimiento y la segunda 48 horas después de la primera. Se sacrificó a las ratas siete días después de la segunda inyección. RESULTADOS:Aunque el uso por separado de MSC derivadas de médula ósea humana (hBM-MSC) o de DFT permitió una resolución parcial del trombo, la combinación de ambos tuvo como resultado la resolución casi completa. La neovascularización fue doblemente mejor en las ratas tratadas con hBM-MSC + DFT (11,6 ± 2,4 canales) en comparación con los grupos asignados por separado a hBM-MSC (3,8 ± 2,7 canales) y DFT (5,5 ± 1,8 canales) (P < 0,0001 y P= 0,002, respectivamente). CONCLUSIÓN:El uso combinado de hBM-MSC y DFT en un modelo de trombosis arterial en ratas mostró que el efecto aditivo tuvo como resultado la resolución casi completa del trombo.
journal_name
Arch Argent Pediatrjournal_title
Archivos argentinos de pediatriaauthors
Dilli D,Kılıç E,Yumuşak N,Beken S,Uçkan Çetinkaya D,Karabulut R,Zenciroğlu ALdoi
10.5546/aap.2017.eng.249subject
Has Abstractpub_date
2017-06-01 00:00:00pages
249-256issue
3eissn
0325-0075issn
1668-3501journal_volume
115pub_type
杂志文章abstract::Combined glomerulopathy is infrequent in pediatric patients. Its presence should be suspected in those patients with glomerulophaties with atypical course. The influence on the long-term renal impairment remains uncertain. Here we report two children with histological findings of combined glomerulopathy. :La combinac...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2018.e688
更新日期:2018-10-01 00:00:00
abstract::The pediatrician is the best-informed professional to whom many families look to be the expert, not only on childhood illnesses but also on development. Early identification, diagnosis and monitoring of these patients are a real challenge for physicians who serve children. This guide provides recommendations for devel...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2017.s53
更新日期:2017-06-01 00:00:00
abstract::Several socio-environmental risk factors causea large number of children born prematurely, and without neurological disorders, to have low scores in their development, particularly in the cognitive and socio-emotional skills areas, thus evidencing a potential future risk. Strengthening bonds with the family and caregi...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2017.eng.588
更新日期:2017-12-01 00:00:00
abstract::There are few studies on the use of illicit drugs during pregnancy with a variable prevalence depending on the year, maternal age, region and diagnostic methods. Mothers' and newborn infants' urine samples were tested for illegal drugs in cases where the mother reported consumption, lack of antenatal care and neonatal...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2013.232
更新日期:2013-06-01 00:00:00
abstract::An adequate drug dosage at treatment initiation is particularly relevant for critically ill patients. An inadequate dosage may result in therapeutic failure, potentially severe adverse events, and unnecessary health expenditures. At present, due to the higher incidence of childhood obesity, primary care physicians are...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2019.eng.e121
更新日期:2019-04-01 00:00:00
abstract::Phelan McDermid Syndrome is caused by the loss of genetic material in a chromosome from pair 22, at the band q13.3. We describe five patients with deletion 22q13.3 in order to establish a genotype-phenotype association, and report the first case described in conjoined twins. We analyzed the perinatal history, psychomo...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2012.e50
更新日期:2012-05-01 00:00:00
abstract:INTRODUCTION:Nutritional status depicts not only the nourishing balance but also the conditions of life. Proper food availability, in form and amount, are aspects of food security. OBJECTIVE:To determine the relationships between hunger perception and stunting. METHOD:Stratified and multiple step sample from nine pro...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.1590/S0325-00752009000300009
更新日期:2009-06-01 00:00:00
abstract:INTRODUCTION:Cow's milk protein allergy is the most common allergy in pediatrics. Immunoglobulin E (IgE)-mediated cow's milk allergy poses a higher risk for life and a worse prognosis. OBJETIVES:To describe the course of IgE-mediated cow's milk protein allergy and risk factors associated with disease persistence. PAT...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2017.eng.331
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:To determine the frequency of the MEFV gene mutations in pediatric patients diagnosed with HSP and to assess the effect of the MEFV gene mutations on their prognosis. Material and Methods. Ccross-sectional study; pediatric patients between 2-11 years diagnosed with HSP were included. These cases were investig...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2018.eng.e385
更新日期:2018-06-01 00:00:00
abstract::In the past two years, different organizations have updated their clinical practice guidelines for hemodynamic support in pediatric septic shock. The studies conducted in adults have questioned the initial management of sepsis in accordance to protocols based on achieving various goals. However, the usefulness of thes...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章,评审
doi:10.5546/aap.2019.eng.e14
更新日期:2019-02-01 00:00:00
abstract::Onychocryptosis (onyx: nail and kriptos: hidden) is the main reason for consultation due to nail pain. It is secondary to the penetration of the lateral border of the nail plate into the lateral nail fold causing pain and inflammation in the surrounding tissue. Symptoms range from erythema, edema, and pain when pressu...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.1590/S0325-00752010000300011
更新日期:2010-06-01 00:00:00
abstract::Central diabetes insipidus is a rare disease in children caused by a deficiency of vasopressin. Its main clinical manifestations are polyuria and polydipsia. Brain malformations are one of the main causes. Desmopressin is the synthetic drug of choice for the treatment. One of the routes of administration is sublingual...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2018.e93
更新日期:2018-02-01 00:00:00
abstract::If newborns have an airway obstruction, they require urgent and expert management to avoid mortality and morbidity. The definition of difficult airway includes problems in endotracheal intubation or positive pressure ventilation with bag and mask or T-piece resuscitator. Management should be based on an understanding ...
journal_title:Archivos argentinos de pediatria
pub_type:
doi:10.5546/aap.2021.e45
更新日期:2021-02-01 00:00:00
abstract::Schwannomas are tumours originating in the Schwann cells of the peripheral nerves. The appearance in the colon is extremely rare, and there are no known cases in the literature of schwannomas of the colon and rectum described in children. These tumours are usually asymptomatic, but can produce non-specific and gastroi...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2019.e68
更新日期:2019-02-01 00:00:00
abstract:INTRODUCTION:In pediatrics, it is decisive to assess the severity of bronchial obstruction; to this end, different clinical scoring scales have been developed, including the modified Tal score. The objective of this study was to determine its validity and reliability in children seen at two emergency departments of Con...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章,多中心研究
doi:10.5546/aap.2019.eng.e340
更新日期:2019-08-01 00:00:00
abstract::Tuberculosis is a common cause of morbimortality among children, especially in developing countries, where 95% of cases occur. Child tuberculosis is closely related to tuberculosis in the adult, since the diagnosis is an indicator of poor surveillance, treatment, and effort to eradicate it. Tuberculous lymphadenitis (...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.1590/S0325-00752011000200012
更新日期:2011-04-01 00:00:00
abstract:INTRODUCTION:Acute respiratory infections (ARI) are a major cause of hospitalization particularly at the extreme ages of life. OBJECTIVES:To determine the viral diagnosis in children < 5 years old with ARI, seasonality, clinical and epidemiological characteristics. POPULATION AND METHODS:A cross-sectional, descriptiv...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章,多中心研究
doi:10.1590/S0325-00752011000400004
更新日期:2011-08-01 00:00:00
abstract::The acid-labile subunit (ALS) is an 85 kDa glycoprotein that belongs to the leucine-rich repeat superfamily. It mainly circulates in serum bound to a high molecular weight ternary complex. The main and most widely studied function of ALS is to prolong the half-life of the binary complex formed by insulin-like growth f...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章,评审
doi:10.5546/aap.2017.eng.391
更新日期:2017-08-01 00:00:00
abstract::This article proposes to define child rearing as a social and cultural process that, far from depicting universal and invariable characteristics, shows a huge diversity, mostly linked to the cultural features of families and communities in charge of such process. It has been considered that the anthropological perspec...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2020.eng.e379
更新日期:2020-08-01 00:00:00
abstract:INTRODUCTION:The analysis of free fetal DNA in maternal plasma allows the assessment of fetal genetic material avoiding the necessity of invasive procedures during pregnancy. OBJECTIVE:To evaluate the feasibility and the diagnostic performance of fetal sex and fetal RhD detection through the analysis of free fetal DNA...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.1590/S0325-00752009000500006
更新日期:2009-10-01 00:00:00
abstract:INTRODUCTION:Children take years to learn symbolic arithmetic. Nevertheless, such as animals and human adults, infants and children can represent approximate number in arrays of objects and sequences of events, and use these capacities to perform approximate addition and subtraction. OBJECTIVE:To evaluate whether pres...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.1590/S0325-00752011000500006
更新日期:2011-10-01 00:00:00
abstract::Carotenemia is generally benign and usually caused by excessive intake of beta-carotene rich food. This clinical presentation can occur in any age group but frequently occurs in children. We report a rare case of carotenemia with an isolated involvement of the nose and presented in order to remind carotenemia in the d...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2019.eng.e502
更新日期:2019-10-01 00:00:00
abstract::Bell's palsy is the most common cause of acquired unilateral facial nerve palsy in childhood. Although the diagnosis depends on the exclusion of less common causes such as infectious, traumatic, malignancy associated and hypertension associated etiologies, pediatricians tend to diagnose idiopatic Bell's palsy whenever...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2013.e133
更新日期:2013-12-01 00:00:00
abstract:INTRODUCTION:Osteomyelitis is uncommon among burn patients. OBJECTIVE:To describe the clinical, microbiological, and evolutionary characteristics of burn children with osteomyelitis hospitalized in a tertiary care facility. METHODS:Retrospective and descriptive study conducted between January 2007 and January 2017. ...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2018.eng.59
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVE:To describe the clinical characteristics of invasive Staphylococcus aureus infections in children and identify the prognostic factors of severity and mortality. MATERIALS AND METHODS:Observational study in patients < 16 years old hospitalized between 2010 and 2015 due to invasive S. aureus infections at the ...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2019.eng.381
更新日期:2019-12-01 00:00:00
abstract:INTRODUCTION:Despite the impact that training in research could have in postgraduate programs, certain specific knowledge of the trainees may increase their scientific production. We described the scientific production of a pediatric residency, and evaluated its association with specific knowledges and activities. MET...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.1590/S0325-00752009000100007
更新日期:2009-02-01 00:00:00
abstract::Health care providers' involvement is critical for quality of care improvement. Nevertheless, most residency programs do not include this topic. The objective is to describe an educational experience on quality of care improvement for residents. The development of an improvement cycle was included in the second year s...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2020.eng.286
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:Hodgkin's lymphoma (HL) is one of the most curable pediatric cancers, however it is rare among children under five years of age and prognostic factors for survival rate are still unknown due to low frequency in this age group. OBJECTIVES:The aim of this study was to evaluate clinical characteristics, treatm...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.5546/aap.2019.eng.e459
更新日期:2019-10-01 00:00:00
abstract::In 2015, there was an increase in the incidence of congenital microcephaly in newborns in Brazil. Months later, the causal relationship between Zika virus and these findings was discovered. In Argentina, during the first outbreak there were 5 cases of congenital Zika syndrome reported. In 2017, there was a new outbrea...
journal_title:Archivos argentinos de pediatria
pub_type:
doi:10.5546/aap.2019.e635
更新日期:2019-12-01 00:00:00
abstract::The characteristic phenotype of CHARGE syndrome includes: coloboma, congenital heart defect, choanal atresia, retarded growth and development, genital abnormalities, ear anomalies with or without hearing loss, which give the name (an acronym) to this condition. The molecular cause in 60% of the cases are mutations in ...
journal_title:Archivos argentinos de pediatria
pub_type: 杂志文章
doi:10.1590/S0325-00752010000100016
更新日期:2010-02-01 00:00:00