Abstract:
BACKGROUND:Polymorphisms at the G72/G30 locus on chromosome 13q have been associated with schizophrenia or bipolar disorder in more than ten independent studies. Even though the genetic findings are very robust, the physiological role of the predicted G72 protein has thus far not been resolved. Initial reports suggested G72 as an activator of D-amino acid oxidase (DAO), supporting the glutamate dysfunction hypothesis of schizophrenia. However, these findings have subsequently not been reproduced and reports of endogenous human G72 mRNA and protein expression are extremely limited. In order to better understand the function of this putative schizophrenia susceptibility gene, we attempted to demonstrate G72 mRNA and protein expression in relevant human brain regions. METHODS:The expression of G72 mRNA was studied by northern blotting and semi-quantitative SYBR-Green and Taqman RT-PCR. Protein expression in human tissue lysates was investigated by western blotting using two custom-made specific anti-G72 peptide antibodies. An in-depth in silico analysis of the G72/G30 locus was performed in order to try and identify motifs or regulatory elements that provide insight to G72 mRNA expression and transcript stability. RESULTS:Despite using highly sensitive techniques, we failed to identify significant levels of G72 mRNA in a variety of human tissues (e.g. adult brain, amygdala, caudate nucleus, fetal brain, spinal cord and testis) human cell lines or schizophrenia/control post mortem BA10 samples. Furthermore, using western blotting in combination with sensitive detection methods, we were also unable to detect G72 protein in a number of human brain regions (including cerebellum and amygdala), spinal cord or testis. A detailed in silico analysis provides several lines of evidence that support the apparent low or absent expression of G72. CONCLUSION:Our results suggest that native G72 protein is not normally present in the tissues that we analysed in this study. We also conclude that the lack of demonstrable G72 expression in relevant brain regions does not support a role for G72 in modulation of DAO activity and the pathology of schizophrenia via a DAO-mediated mechanism. In silico analysis suggests that G72 is not robustly expressed and that the transcript is potentially labile. Further studies are required to understand the significance of the G72/30 locus to schizophrenia.
journal_name
BMC Psychiatryjournal_title
BMC psychiatryauthors
Benzel I,Kew JN,Viknaraja R,Kelly F,de Belleroche J,Hirsch S,Sanderson TH,Maycox PRdoi
10.1186/1471-244X-8-94subject
Has Abstractpub_date
2008-12-11 00:00:00pages
94issn
1471-244Xpii
1471-244X-8-94journal_volume
8pub_type
杂志文章相关文献
BMC Psychiatry文献大全abstract:BACKGROUND:Abnormalities in heart rate during sleep linked to impaired neuro-cardiac modulation may provide new information about physiological sleep signatures of depression. This study assessed the validity of an algorithm using patterns of heart rate changes during sleep to discriminate between individuals with depr...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-019-2152-1
更新日期:2019-06-07 00:00:00
abstract:BACKGROUND:Problematic internet use (PIU) is an increasingly worrisome issue, as youth population studies are establishing links with internalizing and externalizing problems. There is a need for a better understanding of psychiatric diagnostic profiles associated with this issue, as well as its unique contributions to...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-020-02640-x
更新日期:2020-05-27 00:00:00
abstract:BACKGROUND:The aim of the present study was to examine the internal structure and reliability of the Attachment Insecurity Screening Inventory (AISI) 6-12. The AISI 6-12 years is a parent-report questionnaire for assessing the parents' perspective on the quality of the attachment relationship with their child aged betw...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-018-1608-z
更新日期:2018-02-05 00:00:00
abstract:BACKGROUND:Understanding of depression among Vietnamese people living with HIV (PLWH) is limited. This longitudinal study examines changes in depressive symptoms and identifies its correlates among people living with HIV under antiretroviral therapy at An Hoa Clinic. METHODS:People living with HIV ≥18 years and underg...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-016-1170-5
更新日期:2017-01-21 00:00:00
abstract:BACKGROUND:Attitude towards psychiatric patients among healthcare workers has an impact on quality of medical care and rehabilitation of patients. In China, primary healthcare workers play an important role in mental health care, but little is known about the attitude of them towards psychiatric patients. This study ai...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-017-1215-4
更新日期:2017-02-08 00:00:00
abstract:BACKGROUND:While it is recognised that depression is prevalent in Rheumatoid Arthritis (RA), recent studies have also highlighted significant levels of anxiety in RA patients. This study compared two commonly used scales, the Depression Anxiety and Stress Scale (DASS) and the Hospital Anxiety and Depression Scale (HADS...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/1471-244X-12-6
更新日期:2012-01-24 00:00:00
abstract::Following publication of the original article [1], we have been notified that some important information was omitted by the authors from the Competing interests section. The declaration should read as below. ...
journal_title:BMC psychiatry
pub_type: 已发布勘误
doi:10.1186/s12888-019-2207-3
更新日期:2019-07-16 00:00:00
abstract:BACKGROUND:Previous studies focused on the relationship between body mass index and cognitive disorder and obtained many conflicting results. This study explored the potential effects of body mass index on the risk of mild cognitive impairment (amnestic and non-amnestic) in the elderly. METHODS:The study enrolled 240 ...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-017-1493-x
更新日期:2017-09-15 00:00:00
abstract:BACKGROUND:Suicidality has been under-researched in autism spectrum disorders (ASD). Most studies have linked increased suicidality in ASD to psychiatric comorbidities such as depression. Here we investigated, from a neuropsychological and clinical standpoint, the relationship between core ASD symptoms, i.e., restricte...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-019-2122-7
更新日期:2019-04-27 00:00:00
abstract:BACKGROUND:Ego defense mechanisms (or factors), defined by Freud as unconscious resources used by the ego to reduce conflict between the id and superego, are a reflection of how an individual deals with conflict and stress. This study assesses the prevalence of various ego defense mechanisms employed by medical student...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/1471-244X-10-12
更新日期:2010-01-29 00:00:00
abstract:BACKGROUND:Severe mental disorders like psychotic disorders including schizophrenia and schizoaffective disorders have a 12-month-prevalence of 2.6, bipolar disorders of 1.5% in Germany. The relapse risk is high; so many patients need intensive monitoring and lifelong treatment. A high medication adherence is essential...
journal_title:BMC psychiatry
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12888-015-0659-7
更新日期:2015-11-04 00:00:00
abstract:BACKGROUND:Few studies have compared self-reported criminal behaviour with high-quality databases of criminal offences and judicial sanctions. Self-reported problems from drug abusers are generally believed to be valid. We assessed the validity of self-reported theft, drug offences and prison sentences from a five-year...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/1471-244X-8-2
更新日期:2008-01-07 00:00:00
abstract:BACKGROUND:Schizophrenia patients with a metabolically abnormal obese (MAO) phenotype have been shown poor cardiovascular outcomes, but the characteristics of their current psychiatric symptoms have not been characterized. This study mainly explored the psychiatric symptoms of schizophrenia patients with the MAO phenot...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-020-02809-4
更新日期:2020-08-18 00:00:00
abstract:BACKGROUND:The majority of people experiencing mental-health problems do not seek help, and the stigma of mental illness is considered a major barrier to seeking appropriate treatment. More targeted interventions (e.g. at the workplace) seem to be a promising and necessary supplement to public campaigns, but little is ...
journal_title:BMC psychiatry
pub_type: 杂志文章,评审
doi:10.1186/s12888-015-0706-4
更新日期:2016-01-06 00:00:00
abstract:BACKGROUND:Schizotypal personality disorder (SPD) is linked to schizophrenia in terms of shared genetics, biological markers and phenomenological characteristics. In the current study, we aimed to determine whether the previously reported altered functional connectivity (FC) with precuneus in patients with schizophreni...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-016-1146-5
更新日期:2017-02-02 00:00:00
abstract:BACKGROUND:The presence of depressive subsyndromal symptoms (SS) in bipolar disorder (BD) increases the risk of affective relapse and worsens social, cognitive functioning, and quality of life. Nonetheless, there are limited data on how to optimize the treatment of subthreshold depressive symptoms in BD. Mindfulness-Ba...
journal_title:BMC psychiatry
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12888-014-0215-x
更新日期:2014-08-15 00:00:00
abstract:BACKGROUND:Idiopathic pulmonary arterial hypertension (IPAH) is a progressive and fatal cardiovascular disease if left untreated. In patients with IPAH with psychiatric illness or other complications, careful attention is required when administering medical therapies that may affect their hemodynamics. Patients sufferi...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-016-0835-4
更新日期:2016-05-06 00:00:00
abstract:BACKGROUND:Studies of family reactions following teenage suicide are hampered by the psychological difficulties of approaching families and recruiting an unbiased sample of study subjects. By using a small but consecutive series of cases, we examined the qualitative aspects of loosing a teenage family member due to sui...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/1471-244X-8-26
更新日期:2008-04-21 00:00:00
abstract:BACKGROUND:Societies strive for fast-delivered, evidence-based and need-oriented depression treatment within budget constraints. To explore potential improvements, selective contracts can be implemented. Here, we evaluate if the German collaborative psychiatry-neurology-psychotherapy contract (PNP), which extends the g...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-020-02995-1
更新日期:2020-12-14 00:00:00
abstract:BACKGROUND:Benzodiazepine withdrawal syndrome has been reported following attempts to withdraw even from low or therapeutic doses and has been compared to barbiturate and alcohol withdrawal. This experience is known to deter patients from future cessation attempts. Research on other psychotropic substances shows that t...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-015-0493-y
更新日期:2015-05-13 00:00:00
abstract:BACKGROUND:Current clinical guidelines recommend non-pharmacological interventions as first-line treatments for young patients aged 16-24 years with a mental health condition (MHC). However, several studies have noted increasing trends in psychotropic prescribing for this age group, especially in antidepressant prescri...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-017-1375-2
更新日期:2017-06-06 00:00:00
abstract:BACKGROUND:Schizophrenia and bipolar disorder are chronic debilitating disorders that are often treated with second-generation antipsychotic agents, such as aripiprazole, quetiapine, and ziprasidone. While patients who are hospitalized for schizophrenia and bipolar disorder often receive these agents at discharge, comp...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/1471-244X-12-99
更新日期:2012-08-02 00:00:00
abstract:BACKGROUND:Widespread cortical gray matter alternations in people with schizophrenia are correlated with both psychotic symptoms and cognitive/behavioral abnormalities, including the impairments of exploratory eye movement (EEM). Particularly, the loss of gray matter density is specifically related to deficits of the r...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-018-1806-8
更新日期:2018-07-13 00:00:00
abstract:BACKGROUND:We compared the attention abilities of a group of first-episode schizophrenia (FES) patients and a group of healthy participants using the Attention Network Test (ANT), a standard procedure that estimates the functional state of three neural networks controlling the efficiency of three different attentional ...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/1471-244X-12-154
更新日期:2012-09-22 00:00:00
abstract:BACKGROUND:The stimulant methylphenidate (MPH) has been a mainstay of treatment for attention-deficit/hyperactivity disorder (ADHD) for many years. Owing to the short half-life and the issues associated with multiple daily dosing of immediate-release MPH formulations, a new generation of long-acting MPH formulations ha...
journal_title:BMC psychiatry
pub_type: 杂志文章,评审
doi:10.1186/1471-244X-13-237
更新日期:2013-09-27 00:00:00
abstract:BACKGROUND:Treatment disengagement and non-completion poses a major problem for the successful treatment of patients with severe mental illness. Motivation for treatment has long been proposed as a major determinant of treatment engagement, but exact mechanisms remain unclear. This current study serves three purposes: ...
journal_title:BMC psychiatry
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/1471-244X-12-209
更新日期:2012-11-24 00:00:00
abstract:BACKGROUND:Previous studies have provided evidence that alcohol-dependent patients have abnormality in corpus callosum (CC); however, it is unclear whether micro-structural integrity of the CC subregions is differentially affected in this disorder. METHODS:In this study, a total of 39 male individuals, including 19 al...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-019-2079-6
更新日期:2019-03-25 00:00:00
abstract:BACKGROUND:Although the individual and economic disease burden of depression is particularly high for long-term symptoms, little is known of the lifetime course of chronic depression. Most evidence derives from clinical samples, and the diagnostic distinction between persistent depressive disorder (PDD) and non-chronic...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-020-2460-5
更新日期:2020-02-10 00:00:00
abstract:BACKGROUND:Previous studies have demonstrated that individual measures of fitness - such as reduced pulmonary function, slow walking speed and weak handgrip - are associated with an increased risk of dementia. Only a minority of participants included in these studies were aged over 80. The aim of this study was therefo...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-018-1851-3
更新日期:2018-09-04 00:00:00
abstract:BACKGROUND:Executive functioning impairment is common in substance use disorder and is a major risk factor for poor treatment outcomes, including treatment drop-out and relapse. Cognitive remediation interventions seek to improve executive functioning and offer a promising approach to increase the efficacy of alcohol a...
journal_title:BMC psychiatry
pub_type: 杂志文章
doi:10.1186/s12888-019-2044-4
更新日期:2019-02-13 00:00:00