Abstract:
:Intercellular communication mediated by gap junction (GJ) proteins is indispensable during embryogenesis, tissue regeneration and wound healing. Here we report functional analysis of a gap junction protein, Innexin 2 (Inx2), in cell type specification during Drosophila oogenesis. Our data reveal a novel involvement of Inx2 in the specification of Border Cells (BCs), a migratory cell type, whose identity is determined by the cell autonomous STAT activity. We show that Inx2 influences BC fate specification by modulating STAT activity via Domeless receptor endocytosis. Furthermore, detailed experimental analysis has uncovered that Inx2 also regulates a calcium flux that transmits across the follicle cells. We propose that Inx2 mediated calcium flux in the follicle cells stimulates endocytosis by altering Dynamin (Shibire) distribution which is in turn critical for careful calibration of STAT activation and, thus for BC specification. Together our data provide unprecedented molecular insights into how gap junction proteins can regulate cell-type specification.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Sahu A,Ghosh R,Deshpande G,Prasad Mdoi
10.1371/journal.pgen.1006542subject
Has Abstractpub_date
2017-01-23 00:00:00pages
e1006542issue
1eissn
1553-7390issn
1553-7404pii
PGENETICS-D-16-01313journal_volume
13pub_type
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