A monoclonal antibody raised against plasma membrane of cholinergic nerve terminals of the Torpedo inhibits choline acetyltransferase activity and acetylcholine release.

abstract：:The X11-like (X11L) protein was originally isolated as a protein bound to the cytoplasmic domain of the beta-amyloid precursor protein (APP), which is associated with Alzheimer's disease. In mammals, X11L is believed to play an important role in the regulation of APP metabolism. Here we isolated and characterized the ...

journal_title：Journal of neurochemistry

authors： Hase M,Yagi Y,Taru H,Tomita S,Sumioka A,Hori K,Miyamoto K,Sasamura T,Nakamura M,Matsuno K,Suzuki T

更新日期：2002-06-01 00:00:00

abstract：:Nociception evoked prostaglandin (PG) release in the spinal cord considerably contributes to the induction of hyperalgesia and allodynia. To evaluate the relative contribution of cyclooxygenase-1 (COX-1) and COX-2 in this process we assessed the effects of the selective COX-1 inhibitor SC560 and the selective COX-2 in...

journal_title：Journal of neurochemistry

authors： Tegeder I,Niederberger E,Vetter G,Bräutigam L,Geisslinger G

更新日期：2001-11-01 00:00:00

abstract：:Intracellular protein inclusions in Alzheimer's disease and progressive supranuclear palsy contain UBB+1, a variant ubiquitin. UBB+1 is able block the 26S proteasome in cell lines. Proteasome inhibition by drug action has previously been shown to induce a heat-shock response and render protection against stress. We in...

journal_title：Journal of neurochemistry

authors： Hope AD,de Silva R,Fischer DF,Hol EM,van Leeuwen FW,Lees AJ

更新日期：2003-07-01 00:00:00

abstract：:Following a previous report on detection of muscarinic receptors in myelin with the implied presence of G proteins, we now demonstrate by more direct means the presence of such proteins and their quantification. Using [35S]guanosine 5'-O-(3-thiotriphosphate) ([35S]GTP gamma S) as the binding ligand, purified myelin fr...

journal_title：Journal of neurochemistry

authors： Larocca JN,Golly F,Ledeen RW

更新日期：1991-07-01 00:00:00

abstract：:The endocytotic uptake and intracellular decay of horseradish peroxidase isoenzymes C and A by cultured mouse neuroblastoma cells were analyzed quantitatively by a direct spectrophotometric assay. At concentrations below 1 mg/ml, the rate of uptake of the isoenzyme C was more than three times as much as the isoenzyme ...

journal_title：Journal of neurochemistry

authors： Chan KY,Haschke RH

更新日期：1981-01-01 00:00:00

abstract：:Urotensin-II (U-II), a peptide with multiple vascular effects, is detected in cholinergic neurons of the rat brainstem and spinal cord. Here, the effects of U-II on [Ca2+]i was examined in dissociated rat spinal cord neurons by fura 2 microfluorimetry. The neurons investigated were choline acetyltransferase-positive a...

journal_title：Journal of neurochemistry

authors： Filipeanu CM,Brailoiu E,Le Dun S,Dun NJ

更新日期：2002-11-01 00:00:00

abstract：:Tau is a microtubule-associated protein whose transcript undergoes complex regulated splicing in the mammalian nervous system. Exon 6 of the gene is an alternatively spliced cassette whose expression pattern and splicing regulation had not been previously analyzed in the human. The expression profile of exon 6 is comp...

journal_title：Journal of neurochemistry

authors： Wei ML,Andreadis A

更新日期：1998-04-01 00:00:00

abstract：:Brain-derived neurotrophic factor (BDNF) which is primarily associated with neuronal survivability, differentiation and synaptic plasticity has been reported to mediate neurodegeneration in hypoxia through its p75 Neurotrophin receptors (p75NTR). The molecular events promoting BDNF-mediated pro-death signalling in hyp...

journal_title：Journal of neurochemistry

authors： Sharma D,Barhwal KK,Biswal SN,Srivastava AK,Bhardwaj P,Kumar A,Chaurasia OP,Hota SK

更新日期：2019-01-01 00:00:00

abstract：:The effect of docosahexaenoic acid (DHA) on neuronal survival was studied in cultured cells isolated from newborn rat retina. In vivo, the content of DHA in the retina increased nearly fourfold from days 2 to 12 after birth, whereas in retinal cells in culture it remained constant. Unlike amacrine cells, the photorece...

journal_title：Journal of neurochemistry

authors： Rotstein NP,Aveldaño MI,Barrantes FJ,Politi LE

更新日期：1996-05-01 00:00:00

abstract：:Synaptosomal-associated protein of 25 kDa (SNAP-25) is thought to play a key role in vesicle exocytosis and in the control of transmitter release. However, the precise mechanisms of action as well as the regulation of SNAP-25 remain unclear. Here we show by immunoprecipitation that activation of protein kinase C (PKC)...

journal_title：Journal of neurochemistry

authors： Genoud S,Pralong W,Riederer BM,Eder L,Catsicas S,Muller D

更新日期：1999-04-01 00:00:00

abstract：:The mechanism(s) underlying neurodegeneration-associated activation of ERK1/2 remain poorly understood. We report that in cultured rat cortical neurons, whose basal ERK1/2 phosphorylation required NMDA receptors (NMDAR), the neurotoxic DNA intercalating drug cisplatin increased ERK1/2 phosphorylation via NMDAR despite...

journal_title：Journal of neurochemistry

authors： Gozdz A,Vashishta A,Kalita K,Szatmari E,Zheng JJ,Tamiya S,Delamere NA,Hetman M

更新日期：2008-09-01 00:00:00

abstract：:The effect of phorbol esters was investigated on the down-regulation of protein kinase C (PKC) and on the release of [3H]norepinephrine (NE) in synaptosomes from the rat cerebrum. Treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA) promoted the translocation of PKC activity in a P2 fraction from the cytosol to t...

journal_title：Journal of neurochemistry

authors： Oda T,Shearman MS,Nishizuka Y

更新日期：1991-04-01 00:00:00

abstract：:Studies in continuously cultured cells have established that familial Alzheimer's disease (FAD) mutant presenilin 1 (PS1) delays exit of the amyloid precursor protein (APP) from the trans-Golgi network (TGN). Here we report the first description of PS1-regulated APP trafficking in cerebral neurons in culture and in vi...

journal_title：Journal of neurochemistry

authors： Gandy S,Zhang YW,Ikin A,Schmidt SD,Bogush A,Levy E,Sheffield R,Nixon RA,Liao FF,Mathews PM,Xu H,Ehrlich ME

更新日期：2007-08-01 00:00:00

abstract：:Networks of neurons express persistent spontaneous network activity when maintained in dissociated cultures. Prolonged blockade of the spontaneous activity with tetrodotoxin (TTX) causes the eventual death of the neurons. In this study, we investigated some molecular mechanisms that may underlie the activity-suppresse...

journal_title：Journal of neurochemistry

authors： Schonfeld-Dado E,Fishbein I,Segal M

更新日期：2009-08-01 00:00:00

abstract：:Ammonium (NH(4) (+) ) is required to maintain pathways involved in shuttling metabolic precursors between astrocytes and neurones. Under hyperammonaemic conditions, increases in the cellular influx of NH(4) (+) , and accompanying changes in ion concentrations, may contribute to disruptions in metabolism and neurotrans...

journal_title：Journal of neurochemistry

authors： Kelly T,Rose CR

更新日期：2010-12-01 00:00:00

abstract：:Nicotinic cholinergic receptor binding sites labeled by [3H]acetylcholine were measured in the cerebral cortices, thalami, striata, and hypothalami of rats lesioned by intraventricular injection of either 6-hydroxydopamine or 5, 7-dihydroxytryptamine. In addition, [3H]acetylcholine binding sites were measured in the c...

journal_title：Journal of neurochemistry

authors： Schwartz RD,Lehmann J,Kellar KJ

更新日期：1984-05-01 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) was induced in cultured rat astrocytes by incubation with lipopolysaccharide (LPS) for 18 h and gap junction permeability was assessed by the scrape-loading/Lucifer yellow transfer technique. Induction of NOS was confirmed by determining either the NG-methyl-L-arginine (NMMA)-inhibita...

journal_title：Journal of neurochemistry

authors： Bolaños JP,Medina JM

更新日期：1996-05-01 00:00:00

abstract：:Exosomes are small extracellular vesicles released by almost all cell types in physiological and pathological conditions. The exosomal potential to unravel disease mechanisms, or to be used as a source of biomarkers, is being explored, in particularly in the field of neurodegenerative diseases. Alzheimer's disease (AD...

journal_title：Journal of neurochemistry

authors： Soares Martins T,Trindade D,Vaz M,Campelo I,Almeida M,Trigo G,da Cruz E Silva OAB,Henriques AG

更新日期：2020-07-03 00:00:00

abstract：:We examined the effects of cyclic AMP on dopamine receptor-coupled activation of phosphoinositide hydrolysis in rat striatal slices. Forskolin, dibutyryl cyclic AMP, and the protein kinase A activator Sp-cyclic adenosine monophosphothioate (Sp-cAMPS) significantly inhibited inositol phosphate formation stimulated by t...

journal_title：Journal of neurochemistry

authors： Undie AS,Friedman E

更新日期：1994-07-01 00:00:00

abstract：:The metabolism of 6,7-dihydroxy (catecholic)-1,2,3,4-tetrahydroisoquinolines (TIQs) is of interest because the heterocyclic substances may form in mammals normally or during certain disease via condensations of catecholamines (CAs) with aldehydes or alpha-keto acids. With a specific capillary gas chromatography proced...

journal_title：Journal of neurochemistry

authors： Collins MA,Origitano TC

更新日期：1983-12-01 00:00:00

abstract：:To investigate if the prevention of disulfide bond formation affects the intracellular transport, sorting, and processing of a distinct set of neuroendocrine proteins in the regulated secretory pathway, we have treated Xenopus intermediate pituitaries with the thiol-reducing agent dithiothreitol. Pulse-chase incubatio...

journal_title：Journal of neurochemistry

authors： Van Horssen AM,Van Kuppeveld FJ,Martens GJ

更新日期：1998-07-01 00:00:00

abstract：:In vivo microdialysis was used to determine the extent to which ionotropic glutamate receptors in the ventral tegmental area (VTA) regulate dopamine release in the nucleus accumbens. Coapplication of 2-amino-5-phosphonopentanoic acid (AP5; 200 microM) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 50 microM) to the V...

journal_title：Journal of neurochemistry

authors： Taber MT,Das S,Fibiger HC

更新日期：1995-09-01 00:00:00

abstract：:Nucleocytoplasmic transport is a crucial process for cell function. We assessed the general effect of chronic alcohol exposure on this transport in growing astrocytes for the first time. Import and export of proteins to the nucleus were examined by pulse-chase experiments using (3)H-methionine, and we showed that etha...

journal_title：Journal of neurochemistry

authors： Marín MP,Tomas M,Esteban-Pretel G,Megías L,López-Iglesias C,Egea G,Renau-Piqueras J

更新日期：2008-08-01 00:00:00

abstract：:Five-day-old rats subjected to prolonged asphyxia at 37 degrees C show a slower rate of synthesis of 5-HT as measured by the accumulation of the amine after inhibition of monoamine oxidase. During recovery from asphyxia 5-HT synthesis is markedly stimulated when measured over a 50 or 90 min period. No change in 5-HT s...

journal_title：Journal of neurochemistry

authors： Tricklebank MD,Pickard FJ,Dobbing J

更新日期：1976-11-01 00:00:00

abstract：:This study focuses on the potential involvement of carnitine palmitoyltransferase (CPT) on the phospholipid and triglyceride fatty acid turnover in neurons. This category of enzymes, which has been identified in several rat brain tissues, is well known for its role in modulating cellular fatty acid oxidation. Neuronal...

journal_title：Journal of neurochemistry

authors： Arduini A,Denisova N,Virmani A,Avrova N,Federici G,Arrigoni-Martelli E

更新日期：1994-04-01 00:00:00

abstract：:Regional distribution of endogenous gamma-aminobutyric acid (GABA), its synthesizing enzyme, glutamic acid decarboxylase (GAD), and metabolic enzyme, GABA transaminase (GABA-T), were determined in the intestinal tract of guinea pigs and cats and the findings compared with the number of ganglion cells in Auerbach's ple...

journal_title：Journal of neurochemistry

authors： Miki Y,Taniyama K,Tanaka C,Tobe T

更新日期：1983-03-01 00:00:00

abstract：:Calcium-activated neutral proteases (calpains) are regulated by specific endogenous protein inhibitors, the calpastatins, which are widely distributed in mammalian tissues. Calpastatins from different species or in various tissues from the same species exhibit considerable size heterogeneity on sodium dodecyl sulfate ...

journal_title：Journal of neurochemistry

authors： Mohan PS,Nixon RA

更新日期：1995-02-01 00:00:00

abstract：:Oligodendrocytes are glial cells responsible for the synthesis and maintenance of myelin in the central nervous system (CNS). Oligodendrocytes are vulnerable to damage occurring in a variety of neurological diseases. Understanding oligodendrocyte biology is crucial for the dissemination of de- and remyelination mechan...

journal_title：Journal of neurochemistry

authors： Dumont D,Noben JP,Moreels M,Vanderlocht J,Hellings N,Vandenabeele F,Lambrichts I,Stinissen P,Robben J

更新日期：2007-07-01 00:00:00

abstract：:Proteins that control the excitability of neurons, including voltage-dependent ion channels and neurotransmitter receptors, reside in a membrane lipid environment that includes sphingomyelin, but the influence of the metabolism of this lipid on excitability is unknown. Sphingomyelin in the plasma membrane can be cleav...

journal_title：Journal of neurochemistry

authors： Norman E,Cutler RG,Flannery R,Wang Y,Mattson MP

更新日期：2010-07-01 00:00:00

abstract：:We report on the expression of ionotropic glutamate receptor subunits in primary neuronal cultures from rat cortex, hippocampus and cerebellum and of metabotropic glutamate (mGlu) receptor subtypes in these neuronal cultures as well as in cortical astroglial cultures. We found that the NMDA receptor (NR) subunits NR1,...

journal_title：Journal of neurochemistry

authors： Janssens N,Lesage AS

更新日期：2001-06-01 00:00:00