Abstract:
:The objective of this study was to prepare Herceptin (HCT)-functionalized paclitaxel nanocrystals and evaluated their cell-specific interactions, cellular accumulation, and growth inhibition in HER2-positve breast cancer cells as a tumor-targeted delivery module. Paclitaxel (PTX) was fabricated in the form of nanocrystals (PNCs) by a sono-precipitation method, and HCT were coated using a facile non-covalent method (PNCs-HCT). Our results showed that the PNCs-HCT were stable for at least 1month at 4°C with no noticeable desorption of HCT. The release test showed that PNCs-HCT exhibited sustained drug release similar to only PNCs but with a higher release rate than only PTX powder. Cellular uptake, cytotoxicity, and cell cycle arrest studies revealed that PNCs-HCT exhibit greater binding affinity and higher cell-specific internalization to HER2-positive breast cancer cell lines as compared to PNCs, followed by enhanced cell growth inhibition. HCT-functionalized PNCs presented in this study offer a promising strategy for targeted pure drug nanocrystal delivery and enhancing the efficiency of anticancer therapy.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Noh JK,Naeem M,Cao J,Lee EH,Kim MS,Jung Y,Yoo JWdoi
10.1016/j.ijpharm.2016.09.067subject
Has Abstractpub_date
2016-11-20 00:00:00pages
543-553issue
1-2eissn
0378-5173issn
1873-3476pii
S0378-5173(16)30912-7journal_volume
513pub_type
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