Abstract:
:Monoamine oxidases (MAOs) A and B, flavin-containing enzymes found in the outer mitochondrial membrane, oxidize many important biogenic and xenobiotic amines. The two enzymes are expressed in many tissues, with some tissues containing primarily one form and others containing both. Although MAO in placental mitochondria is widely reported to be type A, some investigators have reported low levels of MAO B activity as well. Because placenta is considered the preferred source for purification of type A MAO, we have reinvestigated placental MAO by immunoblotting with monoclonal antibodies and active site labeling with the MAO-specific ligand [3H]pargyline. We have confirmed that placental mitochondrial preparations contain MAO A and low but significant MAO B catalytic activity, as judged by accepted pharmacological criteria (deprenyl-sensitive beta-phenylethylamine and benzylamine oxidation). Immunoblotting revealed polypeptides of sizes expected for both MAO A and B subunits in preparations of placental mitochondria, as well as in preparations of MAO A purified extensively from placenta by partitioning between dextran and polyethylene glycol polymers and chromatography on DEAE-Sepharose CL-6B. Both MAO A and B active sites could be quantitated in placenta by labeling mitochondrial preparations with the MAO-specific affinity ligand [3H] pargyline, followed by immunoprecipitation with MAO A- and MAO B-specific monoclonal antibodies. These results indicate that MAO B activity and protein is consistently present in mitochondrial preparations of human placenta.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Riley LA,Waguespack MA,Denney RMsubject
Has Abstractpub_date
1989-07-01 00:00:00pages
54-60issue
1eissn
0026-895Xissn
1521-0111journal_volume
36pub_type
杂志文章abstract::The presence of DNA damage initiates signaling through the ataxia-telangiectasia mutated kinase (ATM) and the ATM- and the Rad3-related kinase (ATR), which phosphorylate, thus activating, the checkpoint kinases (Chk) 1 and 2, which leads to cell cycle arrest. The bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nit...
journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:10.1124/mol.108.053124
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1124/mol.63.1.2
更新日期:2003-01-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:10.1124/mol.115.099291
更新日期:2015-08-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:10.1124/mol.59.3.462
更新日期:2001-03-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章,收录出版
doi:10.1124/mol.114.092536
更新日期:2014-08-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:10.1124/mol.57.3.589
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:10.1124/mol.110.068122
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:10.1124/mol.104.003269
更新日期:2004-10-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1986-06-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:10.1124/mol.53.3.370
更新日期:1998-03-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章,评审
doi:10.1124/mol.117.110825
更新日期:2018-04-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
doi:10.1124/mol.59.4.939
更新日期:2001-04-01 00:00:00