Characteristics of block by Pb2+ of function of human neuronal L-, N-, and R-type Ca2+ channels transiently expressed in human embryonic kidney 293 cells.

Abstract:

:Lead (Pb(2+)) is a well-known inhibitor of voltage-dependent Ca(2+) channels in their native environments in several types of cells. However, its effects on discrete Ca(2+) channel phenotypes in isolation have not been well studied. We compared how specific subtypes of human neuronal high-voltage-activated Ca(2+) channels were affected by acute exposure to Pb(2+). Expression cDNA clones of human alpha(1C), alpha(1B), or alpha(1E) subunit genes encoding neuronal L-, N-, and R-subtypes of Ca(2+) channels, respectively, along with a constant alpha(2)delta and beta(3) subunits were transfected into human embryonic kidney 293 cells. Currents through the respective transiently expressed channels were measured using whole-cell recording techniques with Ba(2+) (20 mM) as charge carrier. Extracellular bath applications of Pb(2+) significantly reduced current amplitude through all three types of Ca(2+) channels in a concentration-dependent manner. The order of potency was: alpha(1E) (IC(50) = 0.10 microM), followed by alpha(1C) (IC(50) = 0.38 microM) and alpha(1B) (IC(50) = 1.31 microM). Pb(2+)-induced perturbation of function of alpha(1C) and alpha(1B) containing Ca(2+) channels was more easily reversed than for alpha(1E)-containing Ca(2+) channels after washing with Pb(2+) free solution. The current-voltage relationships were not altered after 3-min exposure to Pb(2+) for any of the three types. However, the steady-state inactivation relationships were shifted to more negative potentials for channels containing alpha(1B) and alpha(1E) subunits, but not for those containing alpha(1C) subunits. Pb(2+) accelerated the inactivation time of current in all three subtypes of Ca(2+) channels in a concentration- and voltage-dependent manner. Therefore, different subtypes of Ca(2+) channels exhibit differential susceptibility to Pb(2+) even when expressed in the same cell type. Current expressed by alpha(1E)-containing channels is more sensitive to Pb(2+) than that expressed by alpha(1C)- or alpha(1B)-containing channels. Several Ca(2+) channel phenotypes are quite sensitive to the inhibitory action of Pb(2+). Furthermore, it seems that Pb(2+) is more likely to combine with Ca(2+) channels in the closed state.

journal_name

Mol Pharmacol

journal_title

Molecular pharmacology

authors

Peng S,Hajela RK,Atchison WD

doi

10.1124/mol.62.6.1418

keywords:

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

1418-30

issue

6

eissn

0026-895X

issn

1521-0111

journal_volume

62

pub_type

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