LC-MS/MS method development for quantitative analysis of acetaminophen uptake by the aquatic fungus Mucor hiemalis.

Abstract:

:Acetaminophen is a pharmaceutical, frequently found in surface water as a contaminant. Bioremediation, in particular, mycoremediation of acetaminophen is a method to remove this compound from waters. Owing to the lack of quantitative analytical method for acetaminophen in aquatic organisms, the present study aimed to develop a method for the determination of acetaminophen using LC-MS/MS in the aquatic fungus Mucor hiemalis. The method was then applied to evaluate the uptake of acetaminophen by M. hiemalis, cultured in pellet morphology. The method was robust, sensitive and reproducible with a lower limit of quantification of 5 pg acetaminophen on column. It was found that M. hiemalis internalize the pharmaceutical, and bioaccumulate it with time. Therefore, M. hiemalis was deemed a suitable candidate for further studies to elucidate its pharmaceutical tolerance and the longevity in mycoremediation applications.

journal_name

Ecotoxicol Environ Saf

authors

Esterhuizen-Londt M,Schwartz K,Balsano E,Kühn S,Pflugmacher S

doi

10.1016/j.ecoenv.2016.02.029

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

230-5

eissn

0147-6513

issn

1090-2414

pii

S0147-6513(16)30059-8

journal_volume

128

pub_type

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