Identification and comprehensive analyses of the CBL and CIPK gene families in wheat (Triticum aestivum L.).


BACKGROUND:Calcineurin B-like (CBL) proteins belong to a unique group of calcium sensors in plant that decode the Ca(2+) signature by interacting with CBL-interacting protein kinases (CIPKs). Although CBL-CIPK complexes have been shown to play important roles in the responses to various stresses in plants, little is known about their functions in wheat. RESULTS:A total of seven TaCBL and 20 TaCIPK genes were amplified from bread wheat, Triticum aestivum cv. Chinese Spring. Reverse-transcriptase-polymerase chain reaction (RT-PCR) and in silico expression analyses showed that TaCBL and TaCIPK genes were expressed at different levels in different tissues, or maintained at nearly constant expression levels during the whole life cycle of the wheat plant. Some TaCBL and TaCIPK genes showed up- or down-regulated expressions during seed germination. Preferential interactions between TaCBLs and TaCIPKs were observed in yeast two-hybrid and bimolecular fluorescence complementation experiments. Analyses of a deletion series of TaCIPK proteins with amino acid variations at the C-terminus provided new insights into the specificity of the interactions between TaCIPKs and TaCBLs, and indicated that the TaCBL-TaCIPK signaling pathway is very complex in wheat because of its hexaploid genome. The expressions of many TaCBLs and TaCIPKs were responsive to abiotic stresses (salt, cold, and simulated drought) and abscisic acid treatment. Transgenic Arabidopsis plants overexpressing TaCIPK24 exhibited improved salt tolerance through increased Na(+) efflux and an enhanced reactive oxygen species scavenging capacity. CONCLUSIONS:These results contribute to our understanding of the functions of CBL-CIPK complexes and provide the basis for selecting appropriate genes for in-depth functional studies of CBL-CIPK in wheat.


BMC Plant Biol


BMC plant biology


Sun T,Wang Y,Wang M,Li T,Zhou Y,Wang X,Wei S,He G,Yang G




Has Abstract


2015-11-04 00:00:00










  • AtRabD2b and AtRabD2c have overlapping functions in pollen development and pollen tube growth.

    abstract:BACKGROUND:Rab GTPases are important regulators of endomembrane trafficking, regulating exocytosis, endocytosis and membrane recycling. Many Rab-like proteins exist in plants, but only a subset have been functionally characterized. RESULTS:Here we report that AtRabD2b and AtRabD2c play important roles in pollen develo...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Peng J,Ilarslan H,Wurtele ES,Bassham DC

    更新日期:2011-01-26 00:00:00

  • Correction to: The transcriptome landscape of early maize meiosis.

    abstract:CORRECTION:Following publication of the original article [1], the authors reported that the number of genes overlaying the bar graph in Fig. 3A were incorrectly counted and inserted (i.e. including a title tile, and in inverse order). The corrected numbers are below and match with the listed genes supplied in Additiona...

    journal_title:BMC plant biology

    pub_type: 杂志文章,已发布勘误


    authors: Dukowic-Schulze S,Sundararajan A,Mudge J,Ramaraj T,Farmer AD,Wang M,Sun Q,Pillardy J,Kianian S,Retzel EF,Pawlowski WP,Chen C

    更新日期:2018-01-15 00:00:00

  • Correlation between spatial (3D) structure of pea and bean thylakoid membranes and arrangement of chlorophyll-protein complexes.

    abstract:BACKGROUND:The thylakoid system in plant chloroplasts is organized into two distinct domains: grana arranged in stacks of appressed membranes and non-appressed membranes consisting of stroma thylakoids and margins of granal stacks. It is argued that the reason for the development of appressed membranes in plants is tha...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Rumak I,Mazur R,Gieczewska K,Kozioł-Lipińska J,Kierdaszuk B,Michalski WP,Shiell BJ,Venema JH,Vredenberg WJ,Mostowska A,Garstka M

    更新日期:2012-05-25 00:00:00

  • EDR2 negatively regulates salicylic acid-based defenses and cell death during powdery mildew infections of Arabidopsis thaliana.

    abstract:BACKGROUND:The hypersensitive necrosis response (HR) of resistant plants to avirulent pathogens is a form of programmed cell death in which the plant sacrifices a few cells under attack, restricting pathogen growth into adjacent healthy tissues. In spite of the importance of this defense response, relatively little is ...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Vorwerk S,Schiff C,Santamaria M,Koh S,Nishimura M,Vogel J,Somerville C,Somerville S

    更新日期:2007-07-06 00:00:00

  • Identification of two novel powdery mildew resistance loci, Ren6 and Ren7, from the wild Chinese grape species Vitis piasezkii.

    abstract:BACKGROUND:Grapevine powdery mildew Erysiphe necator is a major fungal disease in all grape growing countries worldwide. Breeding for resistance to this disease is crucial to avoid extensive fungicide applications that are costly, labor intensive and may have detrimental effects on the environment. In the past decade, ...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Pap D,Riaz S,Dry IB,Jermakow A,Tenscher AC,Cantu D,Oláh R,Walker MA

    更新日期:2016-07-29 00:00:00

  • Mapping QTLs and association of differentially expressed gene transcripts for multiple agronomic traits under different nitrogen levels in sorghum.

    abstract:BACKGROUND:Sorghum is an important C4 crop which relies on applied Nitrogen fertilizers (N) for optimal yields, of which substantial amounts are lost into the atmosphere. Understanding the genetic variation of sorghum in response to limited nitrogen supply is important for elucidating the underlying genetic mechanisms ...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Gelli M,Mitchell SE,Liu K,Clemente TE,Weeks DP,Zhang C,Holding DR,Dweikat IM

    更新日期:2016-01-13 00:00:00

  • OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes.

    abstract:BACKGROUND:Drought is a major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors are plant-specific proteins that play diverse roles in plant development. However, the functions of a number of GRAS genes identified in rice are unknown, especially the GRAS genes related to rice dro...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Xu K,Chen S,Li T,Ma X,Liang X,Ding X,Liu H,Luo L

    更新日期:2015-06-13 00:00:00

  • A novel lipid transfer protein from the pea Pisum sativum: isolation, recombinant expression, solution structure, antifungal activity, lipid binding, and allergenic properties.

    abstract:BACKGROUND:Plant lipid transfer proteins (LTPs) assemble a family of small (7-9 kDa) ubiquitous cationic proteins with an ability to bind and transport lipids as well as participate in various physiological processes including defense against phytopathogens. They also form one of the most clinically relevant classes of...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Bogdanov IV,Shenkarev ZO,Finkina EI,Melnikova DN,Rumynskiy EI,Arseniev AS,Ovchinnikova TV

    更新日期:2016-04-30 00:00:00

  • The cellulose synthase superfamily in fully sequenced plants and algae.

    abstract:BACKGROUND:The cellulose synthase superfamily has been classified into nine cellulose synthase-like (Csl) families and one cellulose synthase (CesA) family. The Csl families have been proposed to be involved in the synthesis of the backbones of hemicelluloses of plant cell walls. With 17 plant and algal genomes fully s...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Yin Y,Huang J,Xu Y

    更新日期:2009-07-31 00:00:00

  • An APETALA1 ortholog affects plant architecture and seed yield component in oilseed rape (Brassica napus L.).

    abstract:BACKGROUND:Increasing the productivity of rapeseed as one of the widely cultivated oil crops in the world is of upmost importance. As flowering time and plant architecture play a key role in the regulation of rapeseed yield, understanding the genetic mechanism underlying these traits can boost the rapeseed breeding. Me...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Shah S,Karunarathna NL,Jung C,Emrani N

    更新日期:2018-12-29 00:00:00

  • Identification of small RNAs during cold acclimation in Arabidopsis thaliana.

    abstract:BACKGROUND:Cold stress causes dynamic changes in gene expression that are partially caused by small non-coding RNAs since they regulate protein coding transcripts and act in epigenetic gene silencing pathways. Thus, a detailed analysis of transcriptional changes of small RNAs (sRNAs) belonging to all known sRNA classes...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Tiwari B,Habermann K,Arif MA,Weil HL,Garcia-Molina A,Kleine T,Mühlhaus T,Frank W

    更新日期:2020-06-29 00:00:00

  • A relative quantitative Methylation-Sensitive Amplified Polymorphism (MSAP) method for the analysis of abiotic stress.

    abstract:BACKGROUND:We present a new methylation-sensitive amplified polymorphism (MSAP) approach for the evaluation of relative quantitative characteristics such as demethylation, de novo methylation, and preservation of methylation status of CCGG sequences, which are recognized by the isoschizomers HpaII and MspI. We applied ...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Bednarek PT,Orłowska R,Niedziela A

    更新日期:2017-04-21 00:00:00

  • Characterization of Dehydrin protein, CdDHN4-L and CdDHN4-S, and their differential protective roles against abiotic stress in vitro.

    abstract:BACKGROUND:Dehydrins play positive roles in regulating plant abiotic stress responses. The objective of this study was to characterize two dehydrin genes, CdDHN4-L and CdDHN4-S, generated by alternative splicing of CdDHN4 in bermudagrass. RESULTS:Overexpression of CdDHN4-L with φ-segment and CdDHN4-S lacking of φ-segm...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Lv A,Su L,Liu X,Xing Q,Huang B,An Y,Zhou P

    更新日期:2018-11-26 00:00:00

  • Genome-wide identification and evolutionary analysis of leucine-rich repeat receptor-like protein kinase genes in soybean.

    abstract:BACKGROUND:Leucine-rich repeat receptor-like kinases (LRR-RLKs) constitute the largest subfamily of receptor-like kinases in plant. A number of reports have demonstrated that plant LRR-RLKs play important roles in growth, development, differentiation, and stress responses. However, no comprehensive analysis of this gen...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Zhou F,Guo Y,Qiu LJ

    更新日期:2016-03-02 00:00:00

  • Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance.

    abstract:BACKGROUND:Rice is a chilling-sensitive crop that would suffer serious damage from low temperatures. Overexpression of the Lsi1 gene (Lsi1-OX) in rice enhances its chilling tolerance. This study revealed that a serine hydroxymethyltransferase (OsSHMT) mainly localised in the endoplasmic reticulum (ER) is involved in in...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Fang C,Zhang P,Li L,Yang L,Mu D,Yan X,Li Z,Lin W

    更新日期:2020-05-26 00:00:00

  • Molecular distinction amongst varieties of mulberry using RAPD and DAMD profiles.

    abstract:BACKGROUND:Mulberry trees are the most important host for rearing mulberry silkworms in sericulture. Improved varieties of mulberry tree have been developed through traditional breeding procedures. Not much work, however, has been carried out on the molecular characterization of these varieties. Random Amplified Polymo...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Bhattacharya E,Ranade SA

    更新日期:2001-01-01 00:00:00

  • RNAi-directed downregulation of OsBADH2 results in aroma (2-acetyl-1-pyrroline) production in rice (Oryza sativa L.).

    abstract:BACKGROUND:Aromatic rice is popular worldwide because of its characteristic fragrance. Genetic studies and physical fine mapping reveal that a candidate gene (fgr/OsBADH2) homologous to betaine aldehyde dehydrogenase is responsible for aroma metabolism in fragrant rice varieties, but the direct evidence demonstrating t...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Niu X,Tang W,Huang W,Ren G,Wang Q,Luo D,Xiao Y,Yang S,Wang F,Lu BR,Gao F,Lu T,Liu Y

    更新日期:2008-10-08 00:00:00

  • Identification of plant leaf phosphorus content at different growth stages based on hyperspectral reflectance.

    abstract:BACKGROUND:Modern agriculture strives to sustainably manage fertilizer for both economic and environmental reasons. The monitoring of any nutritional (phosphorus, nitrogen, potassium) deficiency in growing plants is a challenge for precision farming technology. A study was carried out on three species of popular crops,...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Siedliska A,Baranowski P,Pastuszka-Woźniak J,Zubik M,Krzyszczak J

    更新日期:2021-01-07 00:00:00

  • Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data.

    abstract:BACKGROUND:Normalizing through reference genes, or housekeeping genes, can make more accurate and reliable results from reverse transcription real-time quantitative polymerase chain reaction (qPCR). Recent studies have shown that no single housekeeping gene is universal for all experiments. Thus, suitable reference gen...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Artico S,Nardeli SM,Brilhante O,Grossi-de-Sa MF,Alves-Ferreira M

    更新日期:2010-03-21 00:00:00

  • High levels of gene flow and genetic diversity in Irish populations of Salix caprea L. inferred from chloroplast and nuclear SSR markers.

    abstract:BACKGROUND:Salix caprea is a cold-tolerant pioneer species that is ecologically important in Europe and western and central Asia. However, little data is available on its population genetic structure and molecular ecology. We describe the levels of geographic population genetic structure in natural Irish populations of...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Perdereau AC,Kelleher CT,Douglas GC,Hodkinson TR

    更新日期:2014-08-07 00:00:00

  • Genome-wide meta-analysis of maize heterosis reveals the potential role of additive gene expression at pericentromeric loci.

    abstract:BACKGROUND:The identification of QTL involved in heterosis formation is one approach to unravel the not yet fully understood genetic basis of heterosis - the improved agronomic performance of hybrid F1 plants compared to their inbred parents. The identification of candidate genes underlying a QTL is important both for ...

    journal_title:BMC plant biology

    pub_type: 杂志文章,meta分析


    authors: Thiemann A,Fu J,Seifert F,Grant-Downton RT,Schrag TA,Pospisil H,Frisch M,Melchinger AE,Scholten S

    更新日期:2014-04-02 00:00:00

  • Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism.

    abstract:BACKGROUND:The genes of plants can be up- or down-regulated during viral infection to influence the replication of viruses. Identification of these differentially expressed genes could shed light on the defense systems employed by plants and the mechanisms involved in the adaption of viruses to plant cells. Differentia...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Cheng SF,Huang YP,Wu ZR,Hu CC,Hsu YH,Tsai CH

    更新日期:2010-12-27 00:00:00

  • DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks.

    abstract:BACKGROUND:DNA ligase enzymes catalyse the joining of adjacent polynucleotides and as such play important roles in DNA replication and repair pathways. Eukaryotes possess multiple DNA ligases with distinct roles in DNA metabolism, with clear differences in the functions of DNA ligase orthologues between animals, yeast ...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Waterworth WM,Kozak J,Provost CM,Bray CM,Angelis KJ,West CE

    更新日期:2009-06-26 00:00:00

  • Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity.

    abstract:BACKGROUND:Mal d 1 is a major apple allergen causing food allergic symptoms of the oral allergy syndrome (OAS) in birch-pollen sensitised patients. The Mal d 1 gene family is known to have at least 7 intron-containing and 11 intronless members that have been mapped in clusters on three linkage groups. In this study, th...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Gao Z,van de Weg EW,Matos CI,Arens P,Bolhaar ST,Knulst AC,Li Y,Hoffmann-Sommergruber K,Gilissen LJ

    更新日期:2008-11-13 00:00:00

  • Nutritional value of Sesamum indicum L. was improved by Azospirillum and Azotobacter under low input of NP fertilizers.

    abstract:BACKGROUND:Sesame (Sesame indicum L.) is well-known as a versatile industrial crop having various usages and contains 50-55% oil, 20% protein, 14-20% carbohydrate and 2-3% fiber. Several environmental factors are known to adversely affect yield and productivity of sesame. Our overall aim was to improve the growth, yiel...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Nosheen A,Bano A,Naz R,Yasmin H,Hussain I,Ullah F,Keyani R,Hassan MN,Tahir AT

    更新日期:2019-11-04 00:00:00

  • Transcriptomic and proteomic approach to identify differentially expressed genes and proteins in Arabidopsis thaliana mutants lacking chloroplastic 1 and cytosolic FBPases reveals several levels of metabolic regulation.

    abstract:BACKGROUND:During the photosynthesis, two isoforms of the fructose-1,6-bisphosphatase (FBPase), the chloroplastidial (cFBP1) and the cytosolic (cyFBP), catalyse the first irreversible step during the conversion of triose phosphates (TP) to starch or sucrose, respectively. Deficiency in cyFBP and cFBP1 isoforms provokes...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Soto-Suárez M,Serrato AJ,Rojas-González JA,Bautista R,Sahrawy M

    更新日期:2016-12-01 00:00:00

  • Differential transcriptome analysis of glandular and filamentous trichomes in Artemisia annua.

    abstract:BACKGROUND:The medicinal plant Artemisia annua is covered with filamentous trichomes and glandular, artemisinin producing trichomes. A high artemisinin supply is needed at a reduced cost for treating malaria. Artemisinin production in bioreactors can be facilitated if a better insight is obtained in the biosynthesis of...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Soetaert SS,Van Neste CM,Vandewoestyne ML,Head SR,Goossens A,Van Nieuwerburgh FC,Deforce DL

    更新日期:2013-12-20 00:00:00

  • Oligo-painting and GISH reveal meiotic chromosome biases and increased meiotic stability in synthetic allotetraploid Cucumis ×hytivus with dysploid parental karyotypes.

    abstract:BACKGROUND:Meiosis of newly formed allopolyploids frequently encounter perturbations induced by the merging of divergent and hybridizable genomes. However, to date, the meiotic properties of allopolyploids with dysploid parental karyotypes have not been studied in detail. The allotetraploid Cucumis ×hytivus (HHCC, 2n =...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Zhao Q,Wang Y,Bi Y,Zhai Y,Yu X,Cheng C,Wang P,Li J,Lou Q,Chen J

    更新日期:2019-11-06 00:00:00

  • Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves.

    abstract:BACKGROUND:Symptoms of grapevine leafroll disease (GLRD) in red-fruited wine grape (Vitis vinifera L.) cultivars consist of green veins and red and reddish-purple discoloration of inter-veinal areas of leaves. The reddish-purple color of symptomatic leaves may be due to the accumulation of anthocyanins and could reflec...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Gutha LR,Casassa LF,Harbertson JF,Naidu RA

    更新日期:2010-08-23 00:00:00

  • The C-terminal propeptide of a plant defensin confers cytoprotective and subcellular targeting functions.

    abstract:BACKGROUND:Plant defensins are small (45-54 amino acids), basic, cysteine-rich proteins that have a major role in innate immunity in plants. Many defensins are potent antifungal molecules and are being evaluated for their potential to create crop plants with sustainable disease resistance. Defensins are produced as pre...

    journal_title:BMC plant biology

    pub_type: 杂志文章


    authors: Lay FT,Poon S,McKenna JA,Connelly AA,Barbeta BL,McGinness BS,Fox JL,Daly NL,Craik DJ,Heath RL,Anderson MA

    更新日期:2014-02-05 00:00:00