Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs.


:A growing body of evidence suggests that contaminated environments may harbor a greater proportion of antibiotic-resistant microorganisms than unpolluted reference sites. Here, we report the screening of 427 Escherichia coli strains isolated from 11 locations on nine streams draining the US Department of Energy's Savannah River Site against a panel of five antibiotics. Streams were chosen to capture a wide range of watersheds from minimally disturbed to highly impacted. Overall, higher levels of resistance were found in waterborne E. coli that also generally exhibited low spatial variability. However, 3 of 11 locations also demonstrated elevated resistance levels in sediments. Two of these occurred in highly disturbed tributaries with no obvious sources of antimicrobials. To further investigate these patterns, we screened a subset of isolates obtained from three streams against 23 antibiotics or antibiotic combinations. A large proportion of these isolates (>40 %) demonstrated resistance to 10 or more antimicrobials, suggesting that environmental multi-antibiotic resistance may be prevalent in this bacterial commensal. Only 4 of 87 viable isolates were tested susceptible to all 23 antibiotics and combinations. Among these multi-antibiotic-resistant isolates, several demonstrated resistance to all structural classes of antimicrobial agents tested, including frontline antibiotics such as gatifloxacin and ciprofloxacin.


Microb Ecol


Microbial ecology


McArthur JV,Fletcher DE,Tuckfield RC,Baker-Austin C




Has Abstract


2016-11-01 00:00:00














  • Experimental and modeling studies of a four-trophic level predator-prey system.

    abstract::Experimental studies of a microbial food chain involving organic carbon substrates,Enterobacter aerogenes, and ciliate protozoansParamecium primaurelia andDldinium nasutum were conducted in stirred, aerated batch cultures. Quantitative measurements were made of organic carbon levels and of cell numbers, mean cell volu...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Graham JM,Canale RP

    更新日期:1982-12-01 00:00:00

  • Effects of Agaricus lilaceps fairy rings on soil aggregation and microbial community structure in relation to growth stimulation of western wheatgrass (Pascopyrum smithii) in Eastern Montana rangeland.

    abstract::Stimulation of plant productivity caused by Agaricus fairy rings has been reported, but little is known about the effects of these fungi on soil aggregation and the microbial community structure, particularly the communities that can bind soil particles. We studied three concentric zones of Agaricus lilaceps fairy rin...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Caesar-Tonthat TC,Espeland E,Caesar AJ,Sainju UM,Lartey RT,Gaskin JF

    更新日期:2013-07-01 00:00:00

  • Terrestrial Isopods Porcellio scaber and Oniscus asellus (Crustacea: Isopoda) Increase Bacterial Abundance and Modify Microbial Community Structure in Leaf Litter Microcosms: a Short-Term Decomposition Study.

    abstract::Invasive terrestrial isopods are likely to have altered leaf litter decomposition processes in North American forests, but the mechanisms underlying these alterations and the degree to which they differ among isopod species are poorly characterized. Using mixed-deciduous leaf litter microcosms, we quantified the effec...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Des Marteaux LE,Kullik SA,Habash M,Schmidt JM

    更新日期:2020-10-01 00:00:00

  • Soil amendment with Pseudomonas fluorescens CHA0: lasting effects on soil biological properties in soils low in microbial biomass and activity.

    abstract::Pseudomonas fluorescens strains are used in agriculture as plant growth-promoting rhizobacteria (PGPR). Nontarget effects of released organisms should be analyzed prior to their large-scale use, and methods should be available to sensitively detect possible changes in the environments the organism is released to. Acco...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Fliessbach A,Winkler M,Lutz MP,Oberholzer HR,Mäder P

    更新日期:2009-05-01 00:00:00

  • A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization.

    abstract::The diversity and the community structure of sulfate-reducing bacteria (SRB) in an anaerobic continuous bioreactor used for treatment of a sulfate-containing wastewater were investigated by fluorescence in situ hybridization. Hybridization to the 16S rRNA probe EUB338 for the domain Bacteria was performed, followed by...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Icgen B,Moosa S,Harrison ST

    更新日期:2007-01-01 00:00:00

  • Influence of Wastewater Discharge on the Metabolic Potential of the Microbial Community in River Sediments.

    abstract::To reveal the variation of microbial community functions during water filtration process in river sediments, which has been utilized widely in natural water treatment systems, this study investigates the influence of municipal wastewater discharge to streams on the phylotype and metabolic potential of the microbiome i...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Li D,Sharp JO,Drewes JE

    更新日期:2016-01-01 00:00:00

  • Assessing gut microbial diversity from feces and rectal mucosa.

    abstract::Gut microbiota is the most complex bacterial community in the human body and its study may give important clues to the etiology of different intestinal diseases. Most studies carried out so far have used fecal samples, assuming that these samples have a similar distribution to the communities present throughout the co...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Durbán A,Abellán JJ,Jiménez-Hernández N,Ponce M,Ponce J,Sala T,D'Auria G,Latorre A,Moya A

    更新日期:2011-01-01 00:00:00

  • Colonization and Competition Dynamics of Plant Growth-Promoting/Inhibiting Bacteria in the Phytosphere of the Duckweed Lemna minor.

    abstract::Despite the considerable role of aquatic plant-associated bacteria in host plant growth and nutrient cycling in aquatic environments, the mode of their plant colonization has hardly been understood. This study examined the colonization and competition dynamics of a plant growth-promoting bacterium (PGPB) and two plant...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Ishizawa H,Kuroda M,Inoue K,Inoue D,Morikawa M,Ike M

    更新日期:2019-02-01 00:00:00

  • Development and structure of eukaryotic biofilms in an extreme acidic environment, rio tinto (SW, Spain).

    abstract::An in situ colonization assay was performed to study the early stages of biofilm formation in Rio Tinto (SW, Spain), an extremely acidic environment (pH ca. 2). Eukaryotic assemblages were monitored at monthly intervals for 1 year. Diversity, colonization rates, and seasonal variations were analyzed. Structural featur...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Aguilera A,Souza-Egipsy V,Gómez F,Amils R

    更新日期:2007-02-01 00:00:00

  • Influence of Hyphal Inoculum potential on the Competitive Success of Fungi Colonizing Wood.

    abstract::The relative amounts of hyphal inoculum in forest soils may determine the capacity for fungi to compete with and replace early colonizers of wood in ground contact. Our aim in this study was to test the flexibility of priority effects (colonization timing) by varying the timing of inoculum introduction (i.e., precolon...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Song Z,Vail A,Sadowsky MJ,Schilling JS

    更新日期:2015-05-01 00:00:00

  • Diversity of endosymbiotic Nostoc in Gunnera magellanica from Tierra del Fuego, Chile [corrected].

    abstract::Global warming is causing ice retreat in glaciers worldwide, most visibly over the last few decades in some areas of the planet. One of the most affected areas is the region of Tierra del Fuego (southern South America). Vascular plant recolonisation of recently deglaciated areas in this region is initiated by Gunnera ...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Fernández-Martínez MA,de Los Ríos A,Sancho LG,Pérez-Ortega S

    更新日期:2013-08-01 00:00:00

  • Frankia and Alnus rubra canopy roots: an assessment of genetic diversity, propagule availability, and effects on soil nitrogen.

    abstract::The ecological importance of microbial symbioses in terrestrial soils is widely recognized, but their role in soils that accumulate in forest canopies is almost entirely unknown. To address this gap, this study investigated the Frankia-Alnus rubra symbiosis in canopy and forest floor roots at Olympic National Park, WA...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Kennedy PG,Schouboe JL,Rogers RH,Weber MG,Nadkarni NM

    更新日期:2010-02-01 00:00:00

  • The use of phospholipid fatty acid analysis to measure impact of acid rock drainage on microbial communities in sediments.

    abstract::The impact of acid rock drainage (ARD) and eutrophication on microbial communities in stream sediments above and below an abandoned mine site in the Adelaide Hills, South Australia, was quantified by PLFA analysis. Multivariate analysis of water quality parameters, including anions, soluble heavy metals, pH, and condu...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Ben-David EA,Holden PJ,Stone DJ,Harch BD,Foster LJ

    更新日期:2004-10-01 00:00:00

  • Ectoparasite Activity During Incubation Increases Microbial Growth on Avian Eggs.

    abstract::While direct detrimental effects of parasites on hosts are relatively well documented, other more subtle but potentially important effects of parasitism are yet unexplored. Biological activity of ectoparasites, apart from skin injuries and blood-feeding, often results in blood remains, or parasite faeces that accumula...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Tomás G,Martín-Gálvez D,Ruiz-Castellano C,Ruiz-Rodríguez M,Peralta-Sánchez JM,Martín-Vivaldi M,Soler JJ

    更新日期:2018-08-01 00:00:00

  • Effects of temperature and salinity on survival of toxigenicVibrio cholerae O1 in seawater.

    abstract::In 1991 and 1992, the Latin American epidemic strain of Vibrio cholerae O1 was isolated from ballast water, bilge water, and sewage taken from cargo ships docked in Mobile Bay, Alabama. The findings raised questions regarding the organism's ability to survive long-term aboard ships and to withstand the exchange of bal...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: McCarthy SA

    更新日期:1996-03-01 00:00:00

  • Evidence for Niche Differentiation in the Environmental Responses of Co-occurring Mucoromycotinian Fine Root Endophytes and Glomeromycotinian Arbuscular Mycorrhizal Fungi.

    abstract::Fine root endophytes (FRE) were traditionally considered a morphotype of arbuscular mycorrhizal fungi (AMF), but recent genetic studies demonstrate that FRE belong within the subphylum Mucoromycotina, rather than in the subphylum Glomeromycotina with the AMF. These findings prompt enquiry into the fundamental ecology ...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Albornoz FE,Orchard S,Standish RJ,Dickie IA,Bending GD,Hilton S,Lardner T,Foster KJ,Gleeson DB,Bougoure J,Barbetti MJ,You MP,Ryan MH

    更新日期:2020-11-03 00:00:00

  • Fungal growth and biomass development is boosted by plants in snow-covered soil.

    abstract::Soil microbial communities follow distinct seasonal cycles which result in drastic changes in processes involving soil nutrient availability. The biomass of fungi has been reported to be highest during winter, but is fungal growth really occurring in frozen soil? And what is the effect of plant cover on biomass format...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Kuhnert R,Oberkofler I,Peintner U

    更新日期:2012-07-01 00:00:00

  • Viable real-time PCR in environmental samples: can all data be interpreted directly?

    abstract::Selective nucleic acid intercalating dyes--ethidium monoazide (EMA) and propidium monoazide (PMA)--represent one of the most successful recent approaches to detect viable cells (as defined by an intact cell membrane) by PCR and have been effectively evaluated in different microorganisms. However, some practical limita...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Fittipaldi M,Codony F,Adrados B,Camper AK,Morató J

    更新日期:2011-01-01 00:00:00

  • Ciliate Diversity From Aquatic Environments in the Brazilian Atlantic Forest as Revealed by High-Throughput DNA Sequencing.

    abstract::Rainforest aquatic ecosystems include complex habitats with scarce information on their unicellular eukaryote diversity and community structure. We have investigated the diversity of ciliates in freshwater and brackish environments along the Brazilian Atlantic Forest, based on the hypervariable V4 region of the 18S-rD...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Fernandes NM,Campello-Nunes PH,Paiva TS,Soares CAG,Silva-Neto ID

    更新日期:2020-10-06 00:00:00

  • The ecology of testate amoebae (Protists) in sphagnum in North-western Poland in relation to peatland ecology.

    abstract::We studied the relationship between testate amoebae (Protozoa) communities and the depth to the water table (DWT), pH, conductivity, and microhabitat type in Sphagnum dominated peatlands of north-western Poland and built predictive (transfer function) models for inferring DWT and pH based on the testate amoebae commun...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Lamentowicz M,Mitchell EA

    更新日期:2005-07-01 00:00:00

  • Habitat Fragmentation can Modulate Drought Effects on the Plant-soil-microbial System in Mediterranean Holm Oak (Quercus ilex) Forests.

    abstract::Ecological transformations derived from habitat fragmentation have led to increased threats to above-ground biodiversity. However, the impacts of forest fragmentation on soils and their microbial communities are not well understood. We examined the effects of contrasting fragment sizes on the structure and functioning...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Flores-Rentería D,Curiel Yuste J,Rincón A,Brearley FQ,García-Gil JC,Valladares F

    更新日期:2015-05-01 00:00:00

  • Archaeal community structure and pathway of methane formation on rice roots.

    abstract::The community structure of methanogenic Archaea on anoxically incubated rice roots was investigated by amplification, sequencing, and phylogenetic analysis of 16S rRNA and methyl-coenzyme M reductase (mcrA) genes. Both genes demonstrated the presence of Methanomicrobiaceae, Methanobacteriaceae, Methanosarcinaceae, Met...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Chin KJ,Lueders T,Friedrich MW,Klose M,Conrad R

    更新日期:2004-01-01 00:00:00

  • Correction to: Oral Microbial Species and Virulence Factors Associated with Oral Squamous Cell Carcinoma.

    abstract::The original article contained mistakes in the authors' affiliations. ...

    journal_title:Microbial ecology

    pub_type: 已发布勘误


    authors: Torralba MG,Aleti G,Li W,Moncera KJ,Lin YH,Yu Y,Masternak MM,Golusinski W,Golusinski P,Lamperska K,Edlund A,Freire M,Nelson KE

    更新日期:2020-11-20 00:00:00

  • Defining the niche of Vibrio parahaemolyticus during pre- and post-monsoon seasons in the coastal Arabian Sea.

    abstract::The bacteria Vibrio parahaemolyticus is an important component of coastal ecosystems worldwide, and in recent years, V. parahaemolyticus has caused several cases of food-borne gastroenteritis. However, research investigating which parameters are important in regulating V. parahaemolyticus abundance in tropical areas w...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Rehnstam-Holm AS,Atnur V,Godhe A

    更新日期:2014-01-01 00:00:00

  • Laboratory-induced endolithic growth in calcarenites: biodeteriorating potential assessment.

    abstract::This study is aimed to assess the formation of photosynthetic biofilms on and within different natural stone materials, and to analyse their biogeophysical and biogeochemical deterioration potential. This was performed by means of artificial colonisation under laboratory conditions during 3 months. Monitoring of micro...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Miller AZ,Rogerio-Candelera MA,Laiz L,Wierzchos J,Ascaso C,Sequeira Braga MA,Hernández-Mariné M,Maurício A,Dionísio A,Macedo MF,Saiz-Jimenez C

    更新日期:2010-07-01 00:00:00

  • Vibrio harveyi associated with Aglaophenia octodonta (Hydrozoa, Cnidaria).

    abstract::A previously unknown association between a luminous bacterium, Vibrio harveyi, and a benthic hydrozoan, Aglaophenia octodonta, is described. Aglaophenia hydrocladia showed a clear fluorescence in the folds along the hydrocaulus and at the base of the hydrotheca, suggesting the presence of luminous bacteria. This hypot...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Stabili L,Gravili C,Piraino S,Boero F,Alifano P

    更新日期:2006-11-01 00:00:00

  • Culture-dependent and culture-independent analysis of hydrocarbonoclastic microorganisms indigenous to hypersaline environments in Kuwait.

    abstract::The halophilic, hydrocarbonoclastic bacteria and archaea inhabiting two hypersaline coastal areas in Kuwait, one in the north and the other in the south, were counted and characterized. Environmental parameters in both areas were similar, with the exception of the soil organic carbon content, which was in the north hi...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Al-Mailem D,Eliyas M,Khanafer M,Radwan S

    更新日期:2014-05-01 00:00:00

  • Genotypic and phenotypic diversity of cyanobacteria in biological soil crusts of the Succulent Karoo and Nama Karoo of southern Africa.

    abstract::Biological soil crusts (BSCs) are communities of cryptogamic organisms, occurring in arid and semiarid regions all over the world. Based on both morphological identification and genetic analyses, we established a first cyanobacterial inventory using the biphasic approach for BSCs within two major biomes of southern Af...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Dojani S,Kauff F,Weber B,Büdel B

    更新日期:2014-02-01 00:00:00

  • The vertical distribution and diversity of marine bacteriophage at a station off Southern California.

    abstract::Sixty-two bacteriophages were isolated on eight indigenous bacteria from a Pacific Ocean station spanning 887-m vertical depth, on two occasions between 1999 and 2000. On the basis of 16S rRNA sequences, six hosts were tentatively identified to be in the genus Vibrio and the other two were closely related to Altermona...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Jiang S,Fu W,Chu W,Fuhrman JA

    更新日期:2003-05-01 00:00:00

  • Application of nonlinear analysis methods for identifying relationships between microbial community structure and groundwater geochemistry.

    abstract::The relationship between groundwater geochemistry and microbial community structure can be complex and difficult to assess. We applied nonlinear and generalized linear data analysis methods to relate microbial biomarkers (phospholipids fatty acids, PLFA) to groundwater geochemical characteristics at the Shiprock urani...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Schryver JC,Brandt CC,Pfiffner SM,Palumbo AV,Peacock AD,White DC,McKinley JP,Long PE

    更新日期:2006-02-01 00:00:00