Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs.


:A growing body of evidence suggests that contaminated environments may harbor a greater proportion of antibiotic-resistant microorganisms than unpolluted reference sites. Here, we report the screening of 427 Escherichia coli strains isolated from 11 locations on nine streams draining the US Department of Energy's Savannah River Site against a panel of five antibiotics. Streams were chosen to capture a wide range of watersheds from minimally disturbed to highly impacted. Overall, higher levels of resistance were found in waterborne E. coli that also generally exhibited low spatial variability. However, 3 of 11 locations also demonstrated elevated resistance levels in sediments. Two of these occurred in highly disturbed tributaries with no obvious sources of antimicrobials. To further investigate these patterns, we screened a subset of isolates obtained from three streams against 23 antibiotics or antibiotic combinations. A large proportion of these isolates (>40 %) demonstrated resistance to 10 or more antimicrobials, suggesting that environmental multi-antibiotic resistance may be prevalent in this bacterial commensal. Only 4 of 87 viable isolates were tested susceptible to all 23 antibiotics and combinations. Among these multi-antibiotic-resistant isolates, several demonstrated resistance to all structural classes of antimicrobial agents tested, including frontline antibiotics such as gatifloxacin and ciprofloxacin.


Microb Ecol


Microbial ecology


McArthur JV,Fletcher DE,Tuckfield RC,Baker-Austin C




Has Abstract


2016-11-01 00:00:00














  • Microbial diversity inside pumpkins: microhabitat-specific communities display a high antagonistic potential against phytopathogens.

    abstract::Recent and substantial yield losses of Styrian oil pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) are primarily caused by the ascomycetous fungus Didymella bryoniae but bacterial pathogens are frequently involved as well. The diversity of endophytic microbial communities from seeds (spermosphere), roots (...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Fürnkranz M,Lukesch B,Müller H,Huss H,Grube M,Berg G

    更新日期:2012-02-01 00:00:00

  • Minimally Invasive Sampling Method Identifies Differences in Taxonomic Richness of Nasal Microbiomes in Young Infants Associated with Mode of Delivery.

    abstract::To date, there is a limited understanding of the role of the airway microbiome in the early life development of respiratory diseases such as asthma, partly due to a lack of simple and minimally invasive sample collection methods. In order to characterize the baseline microbiome of the upper respiratory tract (URT) in ...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Shilts MH,Rosas-Salazar C,Tovchigrechko A,Larkin EK,Torralba M,Akopov A,Halpin R,Peebles RS,Moore ML,Anderson LJ,Nelson KE,Hartert TV,Das SR

    更新日期:2016-01-01 00:00:00

  • The Oral Bacterium Fusobacterium nucleatum Binds Staphylococcus aureus and Alters Expression of the Staphylococcal Accessory Regulator sarA.

    abstract::Staphylococcus aureus, an opportunistic pathogen member of the nasal and skin microbiota, can also be found in human oral samples and has been linked to infectious diseases of the oral cavity. As the nasal and oral cavities are anatomically connected, it is currently unclear whether S. aureus can colonize the oral cav...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Lima BP,Hu LI,Vreeman GW,Weibel DB,Lux R

    更新日期:2019-08-01 00:00:00

  • A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization.

    abstract::The diversity and the community structure of sulfate-reducing bacteria (SRB) in an anaerobic continuous bioreactor used for treatment of a sulfate-containing wastewater were investigated by fluorescence in situ hybridization. Hybridization to the 16S rRNA probe EUB338 for the domain Bacteria was performed, followed by...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Icgen B,Moosa S,Harrison ST

    更新日期:2007-01-01 00:00:00

  • Ectoparasite Activity During Incubation Increases Microbial Growth on Avian Eggs.

    abstract::While direct detrimental effects of parasites on hosts are relatively well documented, other more subtle but potentially important effects of parasitism are yet unexplored. Biological activity of ectoparasites, apart from skin injuries and blood-feeding, often results in blood remains, or parasite faeces that accumula...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Tomás G,Martín-Gálvez D,Ruiz-Castellano C,Ruiz-Rodríguez M,Peralta-Sánchez JM,Martín-Vivaldi M,Soler JJ

    更新日期:2018-08-01 00:00:00

  • Dissolved organic matter concentration and quality influences upon structure and function of freshwater microbial communities.

    abstract::Past studies have suggested that the concentration and quality of dissolved organic matter (DOM) may influence microbial community structure. In this study, we cross-inoculated the bacterial communities from two streams and a dystrophic lake that varied in DOM concentration and chemistry, to yield nine fully crossed t...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Docherty KM,Young KC,Maurice PA,Bridgham SD

    更新日期:2006-10-01 00:00:00

  • Phylogenetic diversity of actinobacteria associated with soft coral Alcyonium gracllimum and stony coral Tubastraea coccinea in the East China Sea.

    abstract::Actinobacteria are widely distributed in the marine environment. To date, few studies have been performed to explore the coral-associated Actinobacteria, and little is known about the diversity of coral-associated Actinobacteria. In this study, the actinobacterial diversity associated with one soft coral Alcyonium gra...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Yang S,Sun W,Tang C,Jin L,Zhang F,Li Z

    更新日期:2013-07-01 00:00:00

  • Comparative study of nickel toxicity to growth and photosynthesis in nickel-resistant and -sensitive strains of Scenedesmus acutus f. alternans (Chlorophyceae).

    abstract::Nickel (Ni) toxicity to growth and photosynthesis was studied in four strains of Scenedesmus acutus f. alternans. Effects of Ni dosage and duration of exposure on growth and photosynthesis were strain specific. Large differences in responses of both growth and photosynthesis to Ni were detected between three resistant...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Jin X,Nalewajko C,Kushner DJ

    更新日期:1996-01-01 00:00:00

  • Molecular, biochemical, and physiological approaches for understanding the ecology of denitrification.

    abstract::One of the major challenges in microbial ecology for the future is to establish links between structural and functional biodiversity. This is particularly difficult when one is interested in a phylogenetically diversified function such as denitrification. The data banks are very rich in functional gene sequences (nirS...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Goregues CM,Michotey VD,Bonin PC

    更新日期:2005-02-01 00:00:00

  • Penicillium mycobiota in arctic subglacial ice.

    abstract::Fungi have been only rarely isolated from glacial ice in extremely cold polar regions and were in these cases considered as random, long-term preserved Aeolian deposits. Fungal presence has so far not been investigated in polar subglacial ice, a recently discovered extreme habitat reported to be inhabited exclusively ...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Sonjak S,Frisvad JC,Gunde-Cimerman N

    更新日期:2006-08-01 00:00:00

  • Plant Compartments and Developmental Stages Modulate the Balance between Niche-Based and Neutral Processes in Soybean Microbiome.

    abstract::Understanding the dynamics of plant-associated microbial communities within agriculture is well documented. However, the ecological processes that assemble the plant microbiome are not well understood. This study elucidates the relative dominance of assembly processes across plant compartments (root, stem, and leaves)...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Moroenyane I,Mendes L,Tremblay J,Tripathi B,Yergeau É

    更新日期:2021-01-18 00:00:00

  • Response of ammonia oxidizing bacteria and archaea to acute zinc stress and different moisture regimes in soil.

    abstract::Ammonia oxidation has been intensively studied for its sensitivity to environmental shifts and stresses. However, acute stress effects on the occurrence and composition of ammonia oxidizing bacteria (AOB) and archaea (AOA) based on expression of related molecular markers in complex soil environments have been to an ex...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Vasileiadis S,Coppolecchia D,Puglisi E,Balloi A,Mapelli F,Hamon RE,Daffonchio D,Trevisan M

    更新日期:2012-11-01 00:00:00

  • N2 Fixation (C2H 2 reduction) by epiphylls on coffee,Coffea arabica.

    abstract::Nitrogen fixation (C2H2 reduction) by epiphylls on coffee,Coffea arabica, grown in sites with different degrees of shade, was determined. Coffee leaves with nitrogen-fixing epiphylls were found in all sites in approximately equal numbers. Rates of C2H2 reduction were similar for all sites and throughout the year, aver...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Roskoski JP

    更新日期:1980-12-01 00:00:00

  • Penicillin Trunk Injection Affects Bacterial Community Structure in Citrus Trees.

    abstract::Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas), an uncultured α-proteobacterium, is the most destructive disease of citrus trees worldwide. In previous studies, trunk injections of penicillin reduced CLas titers and HLB symptoms in citrus. However, antibiotic effects on the whole plant microbi...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Ascunce MS,Shin K,Huguet-Tapia JC,Poudel R,Garrett KA,van Bruggen AHC,Goss EM

    更新日期:2019-08-01 00:00:00

  • Sediment Microbial Diversity of Three Deep-Sea Hydrothermal Vents Southwest of the Azores.

    abstract::Menez Gwen, Lucky Strike and Rainbow are the three most visited and well-known deep-sea hydrothermal vent fields in the Azores region, located in the Mid-Atlantic Ridge. Their distinct geological and ecological features allow them to support a diversity of vent communities, which are largely dependent on Bacteria and ...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Cerqueira T,Pinho D,Froufe H,Santos RS,Bettencourt R,Egas C

    更新日期:2017-08-01 00:00:00

  • Microbial trophic interactions in aquatic microcosms designed for testing genetically engineered microorganisms: A field comparison.

    abstract::Microcosms may potentially be used as tools for evaluating the fate and effects of genetically engineered microorganisms released into the environment. Extrapolation of data to the field, however, requires that the correspondence between microcosm and field is known. Microbial trophic interactions within the microbial...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Kroer N,Coffin RB

    更新日期:1992-06-01 00:00:00

  • Diversity of Geobacteraceae species inhabiting metal-polluted freshwater lake sediments ascertained by 16S rDNA analyses.

    abstract::The abundance, distribution, and phylogenetic diversity of members of the Fe(III)-reducing family Geobacteraceae were studied along a gradient of metal contaminants in Lake Coeur d'Alene, Idaho. Partial 16S rRNA gene fragments were amplified by PCR using primers directed toward conserved regions of the gene within the...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Cummings DE,Snoeyenbos-West OL,Newby DT,Niggemyer AM,Lovley DR,Achenbach LA,Rosenzweig RF

    更新日期:2003-08-01 00:00:00

  • Bacterial abundance, activity, and viability in the eutrophic River Warnow, northeast Germany.

    abstract::The River Warnow is the drinking water source for the city of Rostock. Its eutrophic status is accompanied by high amounts of bacteria, which may reach up to 24 x 10(6) cells mL(-1) as recorded during a seasonal study in 2002. Because the river is eutrophic and also heavily loaded with organic matter, this burden is a...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Freese HM,Karsten U,Schumann R

    更新日期:2006-01-01 00:00:00

  • Analysis of spatial and temporal variation in the community structure of yeasts associated with decayingOpuntia cactus.

    abstract::The microbial structure within, between, and over time in decaying cladodes of the common prickly pearOpuntia stricta was studied at each of two separate localities. In general, the effective number of yeast species and yeast species diversity increased as the rot aged to the observed maximum time of 4 weeks. Yeast he...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Barker JS,Starmer WT,Vacek DC

    更新日期:1987-11-01 00:00:00

  • Association of rumen ciliate populations with plant particles in vitro.

    abstract::Seven known species of rumen ciliates and mixedEntodinium spp. showed association with plant particles in rumen fluid in vitro. Association was greater with fresh particles than with hay, and substantially decreased when the water-soluble components of the particles were removed, suggesting that the water-soluble comp...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Orpin CG

    更新日期:1985-03-01 00:00:00

  • Biological soil crusts of sand dunes in Cape Cod National Seashore, Massachusetts, USA.

    abstract::Biological soil crusts cover hundreds of hectares of sand dunes at the northern tip of Cape Cod National Seashore (Massachusetts, USA). Although the presence of crusts in this habitat has long been recognized, neither the organisms nor their ecological roles have been described. In this study, we report on the microbi...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Smith SM,Abed RM,Gercia-Pichel F

    更新日期:2004-08-01 00:00:00

  • Response of the sulfate-reducing community to the re-establishment of estuarine conditions in two contrasting soils: a mesocosm approach.

    abstract::We studied the response of the sulfate-reducing prokaryote (SRP) communities to the experimental variation of salinity and tide in an outdoor mesocosm setup. Intact soil monoliths were collected at two areas of the Haringvliet lagoon (The Netherlands): one sampling location consisted of agricultural grassland, drained...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Miletto M,Loeb R,Antheunisse AM,Bodelier PL,Laanbroek HJ

    更新日期:2010-01-01 00:00:00

  • Assessing gut microbial diversity from feces and rectal mucosa.

    abstract::Gut microbiota is the most complex bacterial community in the human body and its study may give important clues to the etiology of different intestinal diseases. Most studies carried out so far have used fecal samples, assuming that these samples have a similar distribution to the communities present throughout the co...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Durbán A,Abellán JJ,Jiménez-Hernández N,Ponce M,Ponce J,Sala T,D'Auria G,Latorre A,Moya A

    更新日期:2011-01-01 00:00:00

  • Fungi from Admiralty Bay (King George Island, Antarctica) Soils and Marine Sediments.

    abstract::Extreme environments such as the Antarctic can lead to the discovery of new microbial taxa, as well as to new microbial-derived natural products. Considering that little is known yet about the diversity and the genetic resources present in these habitats, the main objective of this study was to evaluate the fungal com...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Wentzel LCP,Inforsato FJ,Montoya QV,Rossin BG,Nascimento NR,Rodrigues A,Sette LD

    更新日期:2019-01-01 00:00:00

  • How do Elevated CO2 and Nitrogen Addition Affect Functional Microbial Community Involved in Greenhouse Gas Flux in Salt Marsh System.

    abstract::Salt marshes are unique ecosystem of which a microbial community is expected to be affected by global climate change. In this study, by using T-RFLP analysis, quantitative PCR, and pyrosequencing, we comprehensively analyzed the microbial community structure responding to elevated CO2 (eCO2) and N addition in a salt m...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Lee SH,Megonigal PJ,Kang H

    更新日期:2017-10-01 00:00:00

  • Cysteine-Accelerated Methanogenic Propionate Degradation in Paddy Soil Enrichment.

    abstract::Propionate degradation is a critical step during the conversion of complex organic matter under methanogenic conditions, and it requires a syntrophic cooperation between propionate-oxidizing bacteria and methanogenic archaea. Increasing evidences suggest that interspecies electron transfer for syntrophic metabolism is...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Zhuang L,Ma J,Tang J,Tang Z,Zhou S

    更新日期:2017-05-01 00:00:00

  • Phosphorus Input Alters the Assembly of Rice (Oryza sativa L.) Root-Associated Communities.

    abstract::Rice root-associated microbial community play an important role in plant nutrient acquisition, biomass production, and stress tolerance. Herein, root-associated community assembly was investigated under different phosphate input levels in phosphorus (P)-deficient paddy soil. Rice was grown in a long-term P-depleted pa...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Long XE,Yao H

    更新日期:2020-02-01 00:00:00

  • New insights into the ecological interaction between grape berry microorganisms and Drosophila flies during the development of sour rot.

    abstract::In this work, we studied the ecological interactions between grape berry microorganisms and Drosophila sp. flies involved in sour rot disease during grape ripening. After veráison the total microbial counts of grape berries affected by sour rot increased from about 2 log CFU/g of berries to more than 7 log CFU/g. Berr...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Barata A,Santos SC,Malfeito-Ferreira M,Loureiro V

    更新日期:2012-08-01 00:00:00

  • Spatial and Seasonal Variation in a Reservoir Sedimentary Microbial Community as Determined by Phospholipid Analysis.

    abstract::Sediment samples were collected monthly from Acton Lake, a eutrophic reservoir located in an agricultural region of southwestern Ohio, from three stations (River, Middle, and Dam) during the period May 1995 through January 1997. Sedimentary microbial biomass and community structures from these stations were studied us...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Smoot JC,Findlay RH

    更新日期:2001-10-01 00:00:00

  • A model for the density ofAeromonas hydrophila in Albemarle Sound, North Carolina.

    abstract::The abundance ofAeromonas hydrophila was measured monthly at 29 sites in Albemarle Sound, North Carolina and its tributaries from April 1977 through July 1979. Simultaneous measurements included heterotrophic plate count bacteria, fecal coliform bacteria, and 18 physical and chemical parameters. Using only 6 water qua...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Hazen TC

    更新日期:1983-07-01 00:00:00