A modified method for preparing meiotic chromosomes based on digesting pollen mother cells in suspension.

Abstract:

BACKGROUND:Meiotic chromosome preparation is a key step in plant meiotic research. Pollen mother cell (PMC) wall elimination is beneficial to cytogenetic experimental procedures. Without wall interference, these procedures are easier and more successful. In existing methods it is difficult to eliminate PMC walls completely and uniformly. In this paper, we present an improved method for digesting PMC walls, and one for providing massive chromosomal spreads on a slide for other cytogenetic experimental procedures. RESULTS:Three plants were selected to exhibit the modified meiotic chromosome preparation method. PMCs were dispersed as single cells and incubated in a mixed enzyme solution (3 % cellulose + 0.3 % pectinase + 1 % snailase) for 1.5-2.5 h. In total, 28.28 % cells were lost during this process. There were 800-1900 spreads on every slide and no PMC wall interference was found on any of the slides. The spreads were also evenly distributed on the slides. More spreads were obtained when PMC and protoplast densities in the suspension were increased. All three plants' spreads were successfully used to locate a 5 s rDNA conserved sequence. The Nicotiana hybrid's spreads were successfully used to identify the hybrid's parental genome. CONCLUSION:This is an alternative method for meiotic chromosome preparation. Through this method, PMC walls can be completely and uniformly eliminated, and hundreds of spreads on every slide can be obtained. These spreads can be successfully used for DNA in situ hybridization.

journal_name

Mol Cytogenet

journal_title

Molecular cytogenetics

authors

Dang J,Zhao Q,Yang X,Chen Z,Xiang S,Liang G

doi

10.1186/s13039-015-0184-x

subject

Has Abstract

pub_date

2015-10-24 00:00:00

pages

80

issn

1755-8166

pii

184

journal_volume

8

pub_type

杂志文章
  • Parental origin of deletions and duplications - about the necessity to check for cryptic inversions.

    abstract:Background:Copy number variants (CNVs) are the genetic bases for microdeletion/ microduplication syndromes (MMSs). Couples with an affected child and desire to have further children are routinely tested for a potential parental origin of a specific CNV either by molecular karyotyping or by two color fluorescence in sit...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-018-0369-1

    authors: Liehr T,Schreyer I,Kuechler A,Manolakos E,Singer S,Dufke A,Wilhelm K,Jančušková T,Čmejla R,Othman MAK,Al-Rikabi AH,Mrasek K,Ziegler M,Kankel S,Kreskowski K,Weise A

    更新日期:2018-03-09 00:00:00

  • Cytogenetic analysis in the neotropical fish Astyanax goyacensis Eigenmann, 1908 (Characidae, incertae sedis): karyotype description and occurrence of B microchromosomes.

    abstract:BACKGROUND:B chromosomes, also known as supernumerary or accessory chromosomes, are additional chromosomes over the standard complement found in various groups of plants and animals. We investigated the presence of, and characterized, supernumerary microchromosomes in Astyanax goyacensis using classical and molecular c...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/1755-8166-6-48

    authors: Dos Santos LP,Castro JP,Francisco CM,Vicari MR,de Almeida MC,Goll LG,Morelli S,Artoni RF

    更新日期:2013-11-05 00:00:00

  • Maternal uniparental isodisomy for chromosome 6 discovered by paternity testing: a case report.

    abstract:Background:Uniparental disomy (UPD) is a rare condition in which a child inherits both copies of a chromosome or chromosome segment from one parent. Medical consequences of UPD may include abnormal imprinting, unmasking of genetic disease, and somatic mosaicism; alternatively, the condition may be clinically silent. We...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-018-0411-3

    authors: Kerr ER,Stuhlmiller GM,Maha GC,Ladd MA,Mikhail FM,Koester RP,Hurst ACE

    更新日期:2018-12-20 00:00:00

  • Chromosome 15q13 microduplication in a fetus with cardiac rhabdomyoma: a case report.

    abstract:Background:Copy number variation (CNV) is a complex genomic rearrangement that has been linked to a large number of human diseases. Chromosome 15q13 microduplication is a rare form of CNV, which has been proved to be associated with multiple human disorders; however, the association between chromosome 15q13 microduplic...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-019-0437-1

    authors: Lin CZ,Qi BR,Hu JS,Huang YD,Huang XQ

    更新日期:2019-05-27 00:00:00

  • Phylogenetic insight into subgenera Idaeobatus and Malachobatus (Rubus, Rosaceae) inferring from ISH analysis.

    abstract:BACKGROUND:Rubus is a large and taxonomically complex genus exhibiting agamospermy, polyploidy and frequent hybridization. The objective of this work was to elucidate rDNA disrtibution pattern and investigate genomic composition of polyploids in 16 Rubus taxa (2n = 2x, 3x, 4x, 8x) of two subgenera Idaeobatus and Malach...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-015-0114-y

    authors: Wang Y,Wang X,Chen Q,Zhang L,Tang H,Luo Y,Liu Z

    更新日期:2015-02-03 00:00:00

  • Microarray-based genomic profiling and in situ hybridization on fibrotic bone marrow biopsies for the identification of numerical chromosomal abnormalities in myelodysplastic syndrome.

    abstract:BACKGROUND:Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematological malignancies. In MDS patients with a fibrotic bone marrow the aspiration of cells often fails (dry-tap), which hampers standard karyotyping. Obtaining genetic data from these fibrotic marrows is therefore challenging, and up ti...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-015-0136-5

    authors: Stevens-Kroef MJ,Hebeda KM,Verwiel ET,Kamping EJ,van Cleef PH,Kuiper RP,Groenen PJ

    更新日期:2015-05-28 00:00:00

  • Cytogenetics and stripe rust resistance of wheat-Thinopyrum elongatum hybrid derivatives.

    abstract:Background:Amphidiploids generated by distant hybridization are commonly used as genetic bridge to transfer desirable genes from wild wheat species into cultivated wheat. This method is typically used to enhance the resistance of wheat to biotic or abiotic stresses, and to increase crop yield and quality. Tetraploid Th...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-018-0366-4

    authors: Li D,Long D,Li T,Wu Y,Wang Y,Zeng J,Xu L,Fan X,Sha L,Zhang H,Zhou Y,Kang H

    更新日期:2018-02-05 00:00:00

  • A maternally inherited 8.05 Mb Xq21 deletion associated with Choroideremia, deafness, and mental retardation syndrome in a male patient.

    abstract:BACKGROUND:Deletions in Xq21 cause various congenital defects in males including choroideremia, deafness and mental retardation, depending on their size and gene content. Until now only a limited number of patients with Xq21 deletions has been reported. CASE PRESENTATION:Here we describe a 17-year-old male with choroi...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-017-0324-6

    authors: Liang S,Jiang N,Li S,Jiang X,Yu D

    更新日期:2017-06-14 00:00:00

  • Complex balanced chromosomal translocation t(2;5;13) (p21;p15;q22) in a woman with four reproductive failures.

    abstract:BACKGROUND:Balanced complex translocations (BCTs) are rare events, they may result in reproductive failures: spontaneous abortions, missed abortions, stillbirths, congenital malformations in children, and male infertility. BCTs belong to the group of complex chromosome rearrangements (CCRs) - up to date about 260 cases...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-014-0083-6

    authors: Lazarczyk E,Drozniewska M,Pasinska M,Stasiewicz-Jarocka B,Midro AT,Haus O

    更新日期:2014-11-19 00:00:00

  • De novo mosaic and partial monosomy of chromosome 21 in a case with superior vena cava duplication.

    abstract:Background:Full or partial monosomy of chromosome (chr) 21 is a very rare abnormal cytogenetic finding. It is characterized by variable sizes and deletion breakpoints on the long arm (q) of chr 21 that lead to a broad spectrum of phenotypes that include an increased risk of birth defects, developmental delay and intell...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-020-00513-2

    authors: Azad AK,Yanakakis L,Issleb S,Turina J,Drabik K,Bonner C,Simi E,Wagner A,Fiddler M,Naeem R

    更新日期:2020-09-12 00:00:00

  • Identification of small segmental translocations in patients with repeated implantation failure and recurrent miscarriage using next generation sequencing after in vitro fertilization/intracytoplasmic sperm injection.

    abstract:BACKGROUND:To develop a novel preimplantation genetic screening (PGS) test using next generation sequencing(NGS) as a alternative to current array comparative genomic hybridization (array CGH) method for detection of small segmental translocations in two patients with repeated implantation failure (RIF) and recurrent m...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-015-0207-7

    authors: Ou J,Wang W,Feng T,Liao L,Meng Q,Zou Q,Ding J,Zheng A,Duan C,Li P,Liu Q,Lin C,Li H

    更新日期:2015-12-30 00:00:00

  • A feasible diagnostic approach for the translocation carrier from the indication of products of conception.

    abstract:Background:Chromosome translocations are rare but frequently associated with infertility. The objective of this study is to investigate the feasibility of using chromosomal microarray analysis (CMA) on products of conception (POC) samples as an indicator of parental balanced translocation. From January 2011 to December...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-018-0362-8

    authors: Qian YQ,Fu XY,Wang XQ,Luo YQ,Chen M,Yan K,Yang YM,Liu B,Wang LY,Huang YZ,Li HG,Pan HY,Jin F,Dong MY

    更新日期:2018-01-30 00:00:00

  • Prenatal diagnosis of rearrangements in the fetal 22q11.2 region.

    abstract:Background:22q11.2 deletion syndrome (22q11.2DS) and 22q11.2 duplication syndrome (22q11.2DupS) are the most common copy number variations in humans. The clinical phenotypes of these two syndromes are variable, and there are no large sample data on the prenatal detection rate for these two syndromes in the Chinese popu...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-020-00498-y

    authors: Li S,Jin Y,Yang J,Yang L,Tang P,Zhou C,Wu L,Dong J,Chen J,Shen H

    更新日期:2020-07-08 00:00:00

  • Candidate metastasis suppressor genes uncovered by array comparative genomic hybridization in a mouse allograft model of prostate cancer.

    abstract:BACKGROUND:The purpose of this study was to identify candidate metastasis suppressor genes from a mouse allograft model of prostate cancer (NE-10). This allograft model originally developed metastases by twelve weeks after implantation in male athymic nude mice, but lost the ability to metastasize after a number of in ...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/1755-8166-2-18

    authors: Yi Y,Nandana S,Case T,Nelson C,Radmilovic T,Matusik RJ,Tsuchiya KD

    更新日期:2009-09-26 00:00:00

  • A new case of de novo 19p13.2p13.12 deletion in a girl with overgrowth and severe developmental delay.

    abstract:BACKGROUND:We report clinical and molecular cytogenetic characterization of a 2 year-old girl with 19p13.2p13.12 microdeletion and compare her clinical features with those of three other patients reported before. RESULT:Array comparative genomic hybridization (aCGH) revealed in the present patient a de novo microdelet...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/1755-8166-7-40

    authors: Natiq A,Elalaoui SC,Miesch S,Bonnet C,Jonveaux P,Amzazi S,Sefiani A

    更新日期:2014-06-05 00:00:00

  • Novel complex translocation involving 5 different chromosomes in a chronic myeloid leukemia with Philadelphia chromosome: a case report.

    abstract:BACKGROUND:The well-known typical fusion gene BCR/ABL can be observed in connection with a complex translocation event in only 2-10% of cases with chronic myeloid leukemia (CML). As currently most CML cases are treated with Imatinib, variant rearrangements have in general no specific prognostic significance, though the...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/1755-8166-2-21

    authors: Al Achkar W,Wafa A,Mkrtchyan H,Moassass F,Liehr T

    更新日期:2009-11-09 00:00:00

  • Report of a patient and further clinical and molecular characterization of interstitial 4p16.3 microduplication.

    abstract:BACKGROUND:Pure interstitial duplications of chromosome band 4p16.3 represent an infrequent chromosomal finding with, to the best of our knowledge, only two patients to date reported. CASE PRESENTATION:We report on a 13-year-old boy showing a set of dysmorphic facial features, attention deficit hyperactivity disorders...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-015-0119-6

    authors: Palumbo O,Palumbo P,Ferri E,Riviello FN,Cloroformio L,Carella M,Di Giacomo MC

    更新日期:2015-02-28 00:00:00

  • 3D-FISH analysis reveals chromatid cohesion defect during interphase in Roberts syndrome.

    abstract:BACKGROUND:Roberts syndrome (RBS) is a rare autosomal recessive disorder mainly characterized by growth retardation, limb defects and craniofacial anomalies. Characteristic cytogenetic findings are "railroad track" appearance of chromatids and premature centromere separation in metaphase spreads. Mutations in the ESCO2...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-014-0059-6

    authors: Dupont C,Bucourt M,Guimiot F,Kraoua L,Smiljkovski D,Le Tessier D,Lebugle C,Gerard B,Spaggiari E,Bourdoncle P,Tabet AC,Benzacken B,Dupont JM

    更新日期:2014-09-30 00:00:00

  • De novo duplication of chromosome 16p in a female infant with signs of neonatal hemochromatosis.

    abstract::Reported cases of "pure" duplication of the entire short arm of chromosome 16 (16p) are rare, with only 7 patients described in the literature. We report on a female infant with de novo 16p duplication localized to the short arm of chromosome 6, detected by chromosomal analysis and characterized by array CGH and fluor...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/1755-8166-7-7

    authors: Schwaibold EM,Bartels I,Küster H,Lorenz M,Burfeind P,Adam R,Zoll B

    更新日期:2014-01-23 00:00:00

  • Unexpected structural complexity of supernumerary marker chromosomes characterized by microarray comparative genomic hybridization.

    abstract:BACKGROUND:Supernumerary marker chromosomes (SMCs) are structurally abnormal extra chromosomes that cannot be unambiguously identified by conventional banding techniques. In the past, SMCs have been characterized using a variety of different molecular cytogenetic techniques. Although these techniques can sometimes iden...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/1755-8166-1-7

    authors: Tsuchiya KD,Opheim KE,Hannibal MC,Hing AV,Glass IA,Raff ML,Norwood T,Torchia BA

    更新日期:2008-04-21 00:00:00

  • Kleefstra syndrome in Hungarian patients: additional symptoms besides the classic phenotype.

    abstract:BACKGROUND:Kleefstra syndrome is a rare genetic disorder, with core phenotypic features encompassing developmental delay/intellectual disability, characteristic facial features - brachy(micro)cephaly, unusual shaped eyebrows, flat face with hypertelorism, short nose with anteverted nostrils, thickened lower lip, carpmo...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-016-0231-2

    authors: Hadzsiev K,Komlosi K,Czako M,Duga B,Szalai R,Szabo A,Postyeni E,Szabo T,Kosztolanyi G,Melegh B

    更新日期:2016-02-25 00:00:00

  • Meiotic outcome in two carriers of Y autosome reciprocal translocations: selective elimination of certain segregants.

    abstract:BACKGROUND:Reciprocal Y autosome translocations are rare but frequently associated with male infertility. We report on the meiotic outcome in embryos fathered by two males with the karyotypes 46,X,t(Y;4)(q12;p15.32) and 46,X,t(Y;16)(q12;q13). The two couples underwent preimplantation genetic diagnosis (PGD) enabling de...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-017-0303-y

    authors: Ghevaria H,Naja R,SenGupta S,Serhal P,Delhanty J

    更新日期:2017-02-02 00:00:00

  • Global DNA Methylation patterns on marsupial and devil facial tumour chromosomes.

    abstract:BACKGROUND:Despite DNA methylation being one of the most widely studied epigenetic modifications in eukaryotes, only a few studies have examined the global methylation status of marsupial chromosomes. The emergence of devil facial tumour disease (DFTD), a clonally transmissible cancer spreading through the Tasmanian de...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-015-0176-x

    authors: Ingles ED,Deakin JE

    更新日期:2015-10-01 00:00:00

  • Cytogenetically visible copy number variations (CG-CNVs) in banding and molecular cytogenetics of human; about heteromorphisms and euchromatic variants.

    abstract:BACKGROUND:Copy number variations (CNVs) having no (obvious) clinical effects were rediscovered as major part of human genome in 2004. However, for every cytogeneticist microscopically visible harmless CNVs (CG-CNVs) are well known since decades. Harmless CG-CNVs can be present as heterochromatic or even as euchromatic...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章,评审

    doi:10.1186/s13039-016-0216-1

    authors: Liehr T

    更新日期:2016-01-22 00:00:00

  • GSTM1 copy number variation in the context of single nucleotide polymorphisms in the human GSTM cluster.

    abstract:BACKGROUND:GSTM1 gene deletion is one of the most known copy number polymorphisms in human genome. It is most likely caused by homologous recombination between the repeats flanking the gene. However, taking into account that the deletion has no crucial effects on human well-being, and the ability of other GSTMs to comp...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-016-0241-0

    authors: Khrunin AV,Filippova IN,Aliev AM,Tupitsina TV,Slominsky PA,Limborska SA

    更新日期:2016-04-19 00:00:00

  • A familial chromosomal complex rearrangement confirms RUNX1T1 as a causative gene for intellectual disability and suggests that 1p22.1p21.3 duplication is likely benign.

    abstract:Background:Complex chromosomal rearrangements are constitutive structural aberrations involving three or more breaks. They can be balanced or unbalanced and result in different outcomes, depending on deletion/duplication of genomic material, gene disruption, or position effects. Case presentation:We report on a patien...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-019-0440-6

    authors: Restaldi F,Alesi V,Aquilani A,Genovese S,Russo S,Coletti V,Pompili D,Falasca R,Dallapiccola B,Capolino R,Luciani M,Novelli A

    更新日期:2019-06-14 00:00:00

  • On the significance of germline cytogenetic rearrangements at MYCN locus in neuroblastoma.

    abstract:BACKGROUND:MYCN oncogene amplification is the most important prognostic factor in neuroblastoma. 25% neuroblastoma tumors have somatic amplifications at this locus but little is known about its constitutional aberrations and their potential role in carcinogenesis. Here, we have performed an array-CGH and qPCR character...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/1755-8166-6-43

    authors: Lipska BS,Koczkowska M,Wierzba J,Ploszynska A,Iliszko M,Izycka-Swieszewska E,Adamkiewicz-Drozynska E,Limon J

    更新日期:2013-10-16 00:00:00

  • Microdeletions in 9q33.3-q34.11 in five patients with intellectual disability, microcephaly, and seizures of incomplete penetrance: is STXBP1 not the only causative gene?

    abstract:BACKGROUND:Most microdeletions involving chromosome sub-bands 9q33.3-9q34.11 to this point have been detected by analyses focused on STXBP1, a gene known to cause early infantile epileptic encephalopathy 4 and other seizure phenotypes. Loss-of-function mutations of STXBP1 have also been identified in some patients with...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-015-0178-8

    authors: Ehret JK,Engels H,Cremer K,Becker J,Zimmermann JP,Wohlleber E,Grasshoff U,Rossier E,Bonin M,Mangold E,Bevot A,Schön S,Heilmann-Heimbach S,Dennert N,Mathieu-Dramard M,Lacaze E,Plessis G,de Broca A,Jedraszak G,Röthlis

    更新日期:2015-09-29 00:00:00

  • Chromosomal copy number analysis on chorionic villus samples from early spontaneous miscarriages by high throughput genetic technology.

    abstract:BACKGROUND:About 10 -15 % of all clinically recognized pregnancies result in spontaneous miscarriages, and chromosomal abnormalities are the most common reason. The conventional karyotyping on chorionic villus samples (CVSs) is limited by cell culture and its resolution. This study aimed at evaluating the efficiency of...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章

    doi:10.1186/s13039-015-0210-z

    authors: Shen J,Wu W,Gao C,Ochin H,Qu D,Xie J,Gao L,Zhou Y,Cui Y,Liu J

    更新日期:2016-01-26 00:00:00

  • A rare case of acute promyelocytic leukemia with ider(17)(q10)t(15;17)(q22;q21) and favorable outcome.

    abstract:Background:Chromosomal rearrangements in addition to t(15;17) have been reported in 25-40% of APL patients, with a large predominance of trisomy 8. Other abnormalities are far less frequent, particularly as ider(17), and the prognostic significance is still unclear. Case presentation:We present the case of a patient w...

    journal_title:Molecular cytogenetics

    pub_type:

    doi:10.1186/s13039-020-00479-1

    authors: Liu Y,Xu J,Chu L,Yu L,Zhang Y,Ma L,Wang W,Zhang Y,Xu Y,Liu R

    更新日期:2020-04-10 00:00:00