A modified method for preparing meiotic chromosomes based on digesting pollen mother cells in suspension.


BACKGROUND:Meiotic chromosome preparation is a key step in plant meiotic research. Pollen mother cell (PMC) wall elimination is beneficial to cytogenetic experimental procedures. Without wall interference, these procedures are easier and more successful. In existing methods it is difficult to eliminate PMC walls completely and uniformly. In this paper, we present an improved method for digesting PMC walls, and one for providing massive chromosomal spreads on a slide for other cytogenetic experimental procedures. RESULTS:Three plants were selected to exhibit the modified meiotic chromosome preparation method. PMCs were dispersed as single cells and incubated in a mixed enzyme solution (3 % cellulose + 0.3 % pectinase + 1 % snailase) for 1.5-2.5 h. In total, 28.28 % cells were lost during this process. There were 800-1900 spreads on every slide and no PMC wall interference was found on any of the slides. The spreads were also evenly distributed on the slides. More spreads were obtained when PMC and protoplast densities in the suspension were increased. All three plants' spreads were successfully used to locate a 5 s rDNA conserved sequence. The Nicotiana hybrid's spreads were successfully used to identify the hybrid's parental genome. CONCLUSION:This is an alternative method for meiotic chromosome preparation. Through this method, PMC walls can be completely and uniformly eliminated, and hundreds of spreads on every slide can be obtained. These spreads can be successfully used for DNA in situ hybridization.


Mol Cytogenet


Molecular cytogenetics


Dang J,Zhao Q,Yang X,Chen Z,Xiang S,Liang G




Has Abstract


2015-10-24 00:00:00










  • Cytogenetically visible copy number variations (CG-CNVs) in banding and molecular cytogenetics of human; about heteromorphisms and euchromatic variants.

    abstract:BACKGROUND:Copy number variations (CNVs) having no (obvious) clinical effects were rediscovered as major part of human genome in 2004. However, for every cytogeneticist microscopically visible harmless CNVs (CG-CNVs) are well known since decades. Harmless CG-CNVs can be present as heterochromatic or even as euchromatic...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章,评审


    authors: Liehr T

    更新日期:2016-01-22 00:00:00

  • Microarray-based genomic profiling and in situ hybridization on fibrotic bone marrow biopsies for the identification of numerical chromosomal abnormalities in myelodysplastic syndrome.

    abstract:BACKGROUND:Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematological malignancies. In MDS patients with a fibrotic bone marrow the aspiration of cells often fails (dry-tap), which hampers standard karyotyping. Obtaining genetic data from these fibrotic marrows is therefore challenging, and up ti...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Stevens-Kroef MJ,Hebeda KM,Verwiel ET,Kamping EJ,van Cleef PH,Kuiper RP,Groenen PJ

    更新日期:2015-05-28 00:00:00

  • FISH molecular testing in cytological preparations from solid tumors.

    abstract::Many of the exciting new developments in solid tumor molecular cytogenetics impact classical and molecular pathology. Fluorescence in situ hybridization to identify specific DNA target sequences in nuclei of non-dividing cells in solid neoplasms has contributed to the integration of molecular cytogenetics into cytolog...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Caria P,Vanni R

    更新日期:2014-08-22 00:00:00

  • A rare case of acute promyelocytic leukemia with ider(17)(q10)t(15;17)(q22;q21) and favorable outcome.

    abstract:Background:Chromosomal rearrangements in addition to t(15;17) have been reported in 25-40% of APL patients, with a large predominance of trisomy 8. Other abnormalities are far less frequent, particularly as ider(17), and the prognostic significance is still unclear. Case presentation:We present the case of a patient w...

    journal_title:Molecular cytogenetics



    authors: Liu Y,Xu J,Chu L,Yu L,Zhang Y,Ma L,Wang W,Zhang Y,Xu Y,Liu R

    更新日期:2020-04-10 00:00:00

  • Prolonged exposure to acid and bile induces chromosome abnormalities that precede malignant transformation of benign Barrett's epithelium.

    abstract:UNLABELLED:Barrett's esophagus (BE) is an asymptomatic, pre-malignant condition of the esophagus that can progress to esophageal adenocarcinoma (EAC). BE arises typically in individuals with long-standing gastroesophageal reflux disease (GERD). The neoplastic progression of BE has been extensively studied histologicall...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Bajpai M,Aviv H,Das KM

    更新日期:2012-11-29 00:00:00

  • Overexpression of the proneural transcription factor ASCL1 in chronic lymphocytic leukemia with a t(12;14)(q23.2;q32.3).

    abstract:Background:Translocations of the IGH locus on 14q32.3 are present in about 8% of patients with chronic lymphocytic leukemia (CLL) and contribute to leukemogenesis by deregulating the expression of the IGH-partner genes. Identification of these genes and investigation of the downstream effects of their deregulation can ...

    journal_title:Molecular cytogenetics



    authors: Malli T,Rammer M,Haslinger S,Burghofer J,Burgstaller S,Boesmueller HC,Marschon R,Kranewitter W,Erdel M,Deutschbauer S,Webersinke G

    更新日期:2018-01-11 00:00:00

  • Novel complex translocation involving 5 different chromosomes in a chronic myeloid leukemia with Philadelphia chromosome: a case report.

    abstract:BACKGROUND:The well-known typical fusion gene BCR/ABL can be observed in connection with a complex translocation event in only 2-10% of cases with chronic myeloid leukemia (CML). As currently most CML cases are treated with Imatinib, variant rearrangements have in general no specific prognostic significance, though the...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Al Achkar W,Wafa A,Mkrtchyan H,Moassass F,Liehr T

    更新日期:2009-11-09 00:00:00

  • De novo duplication of chromosome 16p in a female infant with signs of neonatal hemochromatosis.

    abstract::Reported cases of "pure" duplication of the entire short arm of chromosome 16 (16p) are rare, with only 7 patients described in the literature. We report on a female infant with de novo 16p duplication localized to the short arm of chromosome 6, detected by chromosomal analysis and characterized by array CGH and fluor...

    journal_title:Molecular cytogenetics



    authors: Schwaibold EM,Bartels I,Küster H,Lorenz M,Burfeind P,Adam R,Zoll B

    更新日期:2014-01-23 00:00:00

  • A de novo duplication of chromosome 9q34.13-qter in a fetus with Tetralogy of Fallot Syndrome.

    abstract:BACKGROUND:Partial duplications of the distal 9q have been rarely reported in literatures. The key features included characteristic facial appearance, long fingers and toes, slight psychomotor retardation, heart murmur et al. But rare severe congenital heart defects (CHD) such as TOF were reported to be associated with...

    journal_title:Molecular cytogenetics



    authors: Liu J,Hu H,Ma N,Jia Z,Zhou Y,Hu J,Wang H

    更新日期:2016-07-25 00:00:00

  • Heteromorphic variants of chromosome 9.

    abstract:BACKGROUND:Heterochromatic variants of pericentromere of chromosome 9 are reported and discussed since decades concerning their detailed structure and clinical meaning. However, detailed studies are scarce. Thus, here we provide the largest ever done molecular cytogenetic research based on >300 chromosome 9 heteromorph...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Kosyakova N,Grigorian A,Liehr T,Manvelyan M,Simonyan I,Mkrtchyan H,Aroutiounian R,Polityko AD,Kulpanovich AI,Egorova T,Jaroshevich E,Frolova A,Shorokh N,Naumchik IV,Volleth M,Schreyer I,Nelle H,Stumm M,Wegner RD,Rei

    更新日期:2013-04-01 00:00:00

  • Comparative analysis of copy number detection by whole-genome BAC and oligonucleotide array CGH.

    abstract:BACKGROUND:Microarray-based comparative genomic hybridization (aCGH) is a powerful diagnostic tool for the detection of DNA copy number gains and losses associated with chromosome abnormalities, many of which are below the resolution of conventional chromosome analysis. It has been presumed that whole-genome oligonucle...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Neill NJ,Torchia BS,Bejjani BA,Shaffer LG,Ballif BC

    更新日期:2010-06-29 00:00:00

  • Characterization of a prenatally assessed de novo supernumerary minute ring chromosome 20 in a phenotypically normal male.

    abstract:BACKGROUND:The heterogeneous group of small supernumerary marker chromosomes (sSMCs) presents serious counseling problems, especially if they are present de novo and diagnosed prenatally. The incidence has been estimated at 1 in 1000 prenatal samples. We present a case of mosaic sSMC diagnosed prenatally after amniocen...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Kitsiou-Tzeli S,Manolakos E,Lagou M,Kontodiou M,Kosyakova N,Ewers E,Weise A,Garas A,Orru S,Liehr T,Metaxotou A

    更新日期:2009-01-07 00:00:00

  • Report of a patient and further clinical and molecular characterization of interstitial 4p16.3 microduplication.

    abstract:BACKGROUND:Pure interstitial duplications of chromosome band 4p16.3 represent an infrequent chromosomal finding with, to the best of our knowledge, only two patients to date reported. CASE PRESENTATION:We report on a 13-year-old boy showing a set of dysmorphic facial features, attention deficit hyperactivity disorders...

    journal_title:Molecular cytogenetics



    authors: Palumbo O,Palumbo P,Ferri E,Riviello FN,Cloroformio L,Carella M,Di Giacomo MC

    更新日期:2015-02-28 00:00:00

  • Monoallelic and biallelic deletions of 13q14 in a group of CLL/SLL patients investigated by CGH Haematological Cancer and SNP array (8x60K).

    abstract:BACKGROUND:Deletion of 13q14 is the most common cytogenetic change in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and is detected in about 50 % of patients by fluorescence in situ hybridization (FISH), which can reveal presence of del(13)(q14) and mono- or biallelic deletion status without informa...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Grygalewicz B,Woroniecka R,Rygier J,Borkowska K,Rzepecka I,Łukasik M,Budziłowska A,Rymkiewicz G,Błachnio K,Nowakowska B,Bartnik M,Gos M,Pieńkowska-Grela B

    更新日期:2016-01-06 00:00:00

  • Hyperdiploidy associated with T315I mutation in BCR-ABL kinase domain in an accelerated phase-chronic myeloid leukemia case.

    abstract:BACKGROUND:Chronic myeloid leukemia (CML) is genetically characterized by the occurrence of a reciprocal translocation t(9;22)(q34;q11), resulting in a BCR/ABL gene fusion on the derivative chromosome 22, i.e. the Philadelphia (Ph) chromosome. During CML progression 60-80% of the cases acquire additional genetic change...

    journal_title:Molecular cytogenetics



    authors: Al-Achkar W,Moassass F,Ikhtiar A,Liehr T,Othman MA,Wafa A

    更新日期:2014-11-29 00:00:00

  • Partial monosomy14q involving FOXG1 and NOVA1 in an infant with microcephaly, seizures and severe developmental delay.

    abstract:BACKGROUND:FOXG1 gene mutations have been associated with the congenital variant of Rett syndrome (RTT) since the initial description of two patients in 2008. The on-going accumulation of clinical data suggests that the FOXG1-variant of RTT forms a distinguishable phenotype, consisting mainly of postnatal microcephaly,...

    journal_title:Molecular cytogenetics



    authors: Fryssira H,Tsoutsou E,Psoni S,Amenta S,Liehr T,Anastasakis E,Skentou Ch,Ntouflia A,Papoulidis I,Manolakos E,Chaliasos N

    更新日期:2016-08-02 00:00:00

  • 13q deletion syndrome resulting from balanced chromosomal rearrangement in father: the significance of parental karyotyping.

    abstract:Background:Retinoblastoma is a malignancy of the eye in children characterized by biallelic inactivation of the retinoblastoma 1 gene (RB1), located at chromosome 13q14.2. Children with interstitial chromosome 13q deletions that include the RB1 gene show a predisposition to develop retinoblastoma and variable other fea...

    journal_title:Molecular cytogenetics



    authors: Dittner-Moormann S,Reschke M,Biewald E,Kuechler A,Klein B,Timmermann B,Lohmann D,Ketteler P,Kanber D

    更新日期:2020-07-23 00:00:00

  • Cytogenetics and stripe rust resistance of wheat-Thinopyrum elongatum hybrid derivatives.

    abstract:Background:Amphidiploids generated by distant hybridization are commonly used as genetic bridge to transfer desirable genes from wild wheat species into cultivated wheat. This method is typically used to enhance the resistance of wheat to biotic or abiotic stresses, and to increase crop yield and quality. Tetraploid Th...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Li D,Long D,Li T,Wu Y,Wang Y,Zeng J,Xu L,Fan X,Sha L,Zhang H,Zhou Y,Kang H

    更新日期:2018-02-05 00:00:00

  • Candidate metastasis suppressor genes uncovered by array comparative genomic hybridization in a mouse allograft model of prostate cancer.

    abstract:BACKGROUND:The purpose of this study was to identify candidate metastasis suppressor genes from a mouse allograft model of prostate cancer (NE-10). This allograft model originally developed metastases by twelve weeks after implantation in male athymic nude mice, but lost the ability to metastasize after a number of in ...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Yi Y,Nandana S,Case T,Nelson C,Radmilovic T,Matusik RJ,Tsuchiya KD

    更新日期:2009-09-26 00:00:00

  • Chromosome 15q13 microduplication in a fetus with cardiac rhabdomyoma: a case report.

    abstract:Background:Copy number variation (CNV) is a complex genomic rearrangement that has been linked to a large number of human diseases. Chromosome 15q13 microduplication is a rare form of CNV, which has been proved to be associated with multiple human disorders; however, the association between chromosome 15q13 microduplic...

    journal_title:Molecular cytogenetics



    authors: Lin CZ,Qi BR,Hu JS,Huang YD,Huang XQ

    更新日期:2019-05-27 00:00:00

  • De novo mosaic and partial monosomy of chromosome 21 in a case with superior vena cava duplication.

    abstract:Background:Full or partial monosomy of chromosome (chr) 21 is a very rare abnormal cytogenetic finding. It is characterized by variable sizes and deletion breakpoints on the long arm (q) of chr 21 that lead to a broad spectrum of phenotypes that include an increased risk of birth defects, developmental delay and intell...

    journal_title:Molecular cytogenetics



    authors: Azad AK,Yanakakis L,Issleb S,Turina J,Drabik K,Bonner C,Simi E,Wagner A,Fiddler M,Naeem R

    更新日期:2020-09-12 00:00:00

  • Global DNA Methylation patterns on marsupial and devil facial tumour chromosomes.

    abstract:BACKGROUND:Despite DNA methylation being one of the most widely studied epigenetic modifications in eukaryotes, only a few studies have examined the global methylation status of marsupial chromosomes. The emergence of devil facial tumour disease (DFTD), a clonally transmissible cancer spreading through the Tasmanian de...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Ingles ED,Deakin JE

    更新日期:2015-10-01 00:00:00

  • Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species.

    abstract:BACKGROUND:The uncultivated species Dasypyrum breviaristatum carries novel diseases resistance and agronomically important genes of potential use for wheat improvement. The development of new wheat-D. breviaristatum derivatives lines with disease resistance provides an opportunity for the identification and localizatio...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Li G,Gao D,Zhang H,Li J,Wang H,La S,Ma J,Yang Z

    更新日期:2016-01-25 00:00:00

  • A new case of de novo 19p13.2p13.12 deletion in a girl with overgrowth and severe developmental delay.

    abstract:BACKGROUND:We report clinical and molecular cytogenetic characterization of a 2 year-old girl with 19p13.2p13.12 microdeletion and compare her clinical features with those of three other patients reported before. RESULT:Array comparative genomic hybridization (aCGH) revealed in the present patient a de novo microdelet...

    journal_title:Molecular cytogenetics



    authors: Natiq A,Elalaoui SC,Miesch S,Bonnet C,Jonveaux P,Amzazi S,Sefiani A

    更新日期:2014-06-05 00:00:00

  • Cytogenetic analysis in the neotropical fish Astyanax goyacensis Eigenmann, 1908 (Characidae, incertae sedis): karyotype description and occurrence of B microchromosomes.

    abstract:BACKGROUND:B chromosomes, also known as supernumerary or accessory chromosomes, are additional chromosomes over the standard complement found in various groups of plants and animals. We investigated the presence of, and characterized, supernumerary microchromosomes in Astyanax goyacensis using classical and molecular c...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Dos Santos LP,Castro JP,Francisco CM,Vicari MR,de Almeida MC,Goll LG,Morelli S,Artoni RF

    更新日期:2013-11-05 00:00:00

  • Automated detection of residual cells after sex-mismatched stem-cell transplantation - evidence for presence of disease-marker negative residual cells.

    abstract:BACKGROUND:A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.Cells of 58 patients were characterized as disease-associated due to presence of a ...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Erlecke J,Hartmann I,Hoffmann M,Kroll T,Starke H,Heller A,Gloria A,Sayer HG,Johannes T,Claussen U,Liehr T,Loncarevic IF

    更新日期:2009-05-29 00:00:00

  • Chromothripsis 18 in multiple myeloma patient with rapid extramedullary relapse.

    abstract:Background:Catastrophic chromosomal event known as chromothripsis was proven to be a significant hallmark of poor prognosis in several cancer diseases. While this phenomenon is very rare in among multiple myeloma (MM) patients, its presence in karyotype is associated with very poor prognosis. Case presentation:In our ...

    journal_title:Molecular cytogenetics



    authors: Smetana J,Oppelt J,Štork M,Pour L,Kuglík P

    更新日期:2018-01-18 00:00:00

  • Kleefstra syndrome in Hungarian patients: additional symptoms besides the classic phenotype.

    abstract:BACKGROUND:Kleefstra syndrome is a rare genetic disorder, with core phenotypic features encompassing developmental delay/intellectual disability, characteristic facial features - brachy(micro)cephaly, unusual shaped eyebrows, flat face with hypertelorism, short nose with anteverted nostrils, thickened lower lip, carpmo...

    journal_title:Molecular cytogenetics



    authors: Hadzsiev K,Komlosi K,Czako M,Duga B,Szalai R,Szabo A,Postyeni E,Szabo T,Kosztolanyi G,Melegh B

    更新日期:2016-02-25 00:00:00

  • DNA secondary structure is influenced by genetic variation and alters susceptibility to de novo translocation.

    abstract:BACKGROUND:Cumulative evidence suggests that DNA secondary structures impact DNA replication, transcription and genomic rearrangements. One of the best studied examples is the recurrent constitutional t(11;22) in humans that is mediated by potentially cruciform-forming sequences at the breakpoints, palindromic AT-rich ...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Kato T,Inagaki H,Tong M,Kogo H,Ohye T,Yamada K,Tsutsumi M,Emanuel BS,Kurahashi H

    更新日期:2011-09-08 00:00:00

  • On the significance of germline cytogenetic rearrangements at MYCN locus in neuroblastoma.

    abstract:BACKGROUND:MYCN oncogene amplification is the most important prognostic factor in neuroblastoma. 25% neuroblastoma tumors have somatic amplifications at this locus but little is known about its constitutional aberrations and their potential role in carcinogenesis. Here, we have performed an array-CGH and qPCR character...

    journal_title:Molecular cytogenetics

    pub_type: 杂志文章


    authors: Lipska BS,Koczkowska M,Wierzba J,Ploszynska A,Iliszko M,Izycka-Swieszewska E,Adamkiewicz-Drozynska E,Limon J

    更新日期:2013-10-16 00:00:00