当前位置： SCI文献检索 > Molecular Cytogenetics期刊下所有文献 > A modified method for preparing meiotic chromosomes based on digesting pollen mother cells in suspension.

A modified method for preparing meiotic chromosomes based on digesting pollen mother cells in suspension.

Abstract：

BACKGROUND:Meiotic chromosome preparation is a key step in plant meiotic research. Pollen mother cell (PMC) wall elimination is beneficial to cytogenetic experimental procedures. Without wall interference, these procedures are easier and more successful. In existing methods it is difficult to eliminate PMC walls completely and uniformly. In this paper, we present an improved method for digesting PMC walls, and one for providing massive chromosomal spreads on a slide for other cytogenetic experimental procedures. RESULTS:Three plants were selected to exhibit the modified meiotic chromosome preparation method. PMCs were dispersed as single cells and incubated in a mixed enzyme solution (3 % cellulose + 0.3 % pectinase + 1 % snailase) for 1.5-2.5 h. In total, 28.28 % cells were lost during this process. There were 800-1900 spreads on every slide and no PMC wall interference was found on any of the slides. The spreads were also evenly distributed on the slides. More spreads were obtained when PMC and protoplast densities in the suspension were increased. All three plants' spreads were successfully used to locate a 5 s rDNA conserved sequence. The Nicotiana hybrid's spreads were successfully used to identify the hybrid's parental genome. CONCLUSION:This is an alternative method for meiotic chromosome preparation. Through this method, PMC walls can be completely and uniformly eliminated, and hundreds of spreads on every slide can be obtained. These spreads can be successfully used for DNA in situ hybridization.

journal_name

Mol Cytogenet

journal_title

Molecular cytogenetics

authors

Dang J,Zhao Q,Yang X,Chen Z,Xiang S,Liang G

doi

10.1186/s13039-015-0184-x

subject

Has Abstract

pub_date

2015-10-24 00:00:00

pages

80

issn

1755-8166

pii

184

journal_volume

8

pub_type

杂志文章

相关文献

Molecular Cytogenetics文献大全
  • Rare gene fusion rearrangement SPTNB1-PDGFRB in an atypical myeloproliferative neoplasm.

    abstract：:The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia recognizes a distinct class of myeloid and lymphoid tumors with eosinophilia-related proliferations associated with specific gene rearrangements, one of which involves rearrangements of platelet-derived growth fac...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/s13039-018-0405-1

    authors： Furtado VF,Saini NY,Walsh W,Bathini V,Miron PM

    更新日期：2018-10-19 00:00:00

  • A maternally inherited 8.05 Mb Xq21 deletion associated with Choroideremia, deafness, and mental retardation syndrome in a male patient.

    abstract：BACKGROUND:Deletions in Xq21 cause various congenital defects in males including choroideremia, deafness and mental retardation, depending on their size and gene content. Until now only a limited number of patients with Xq21 deletions has been reported. CASE PRESENTATION:Here we describe a 17-year-old male with choroi...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-017-0324-6

    authors： Liang S,Jiang N,Li S,Jiang X,Yu D

    更新日期：2017-06-14 00:00:00

  • Chromothripsis 18 in multiple myeloma patient with rapid extramedullary relapse.

    abstract：Background:Catastrophic chromosomal event known as chromothripsis was proven to be a significant hallmark of poor prognosis in several cancer diseases. While this phenomenon is very rare in among multiple myeloma (MM) patients, its presence in karyotype is associated with very poor prognosis. Case presentation:In our ...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-018-0357-5

    authors： Smetana J,Oppelt J,Štork M,Pour L,Kuglík P

    更新日期：2018-01-18 00:00:00

  • Partial monosomy14q involving FOXG1 and NOVA1 in an infant with microcephaly, seizures and severe developmental delay.

    abstract：BACKGROUND:FOXG1 gene mutations have been associated with the congenital variant of Rett syndrome (RTT) since the initial description of two patients in 2008. The on-going accumulation of clinical data suggests that the FOXG1-variant of RTT forms a distinguishable phenotype, consisting mainly of postnatal microcephaly,...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-016-0269-1

    authors： Fryssira H,Tsoutsou E,Psoni S,Amenta S,Liehr T,Anastasakis E,Skentou Ch,Ntouflia A,Papoulidis I,Manolakos E,Chaliasos N

    更新日期：2016-08-02 00:00:00

  • Report of a patient and further clinical and molecular characterization of interstitial 4p16.3 microduplication.

    abstract：BACKGROUND:Pure interstitial duplications of chromosome band 4p16.3 represent an infrequent chromosomal finding with, to the best of our knowledge, only two patients to date reported. CASE PRESENTATION:We report on a 13-year-old boy showing a set of dysmorphic facial features, attention deficit hyperactivity disorders...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-015-0119-6

    authors： Palumbo O,Palumbo P,Ferri E,Riviello FN,Cloroformio L,Carella M,Di Giacomo MC

    更新日期：2015-02-28 00:00:00

  • Genomic instability in a chronic lymphocytic leukemia patient with mono-allelic deletion of the DLEU and RB1 genes.

    abstract：Background:The most frequent cytogenetic abnormality detected in chronic lymphocytic leukemia (CLL) patients is the presence of a deletion within the chromosome band 13q14. Deletions can be heterogeneous in size, generally encompassing the DLEU1 and DLEU2 genes (minimal deleted region), but at times also including the ...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-019-0417-5

    authors： Nava-Rodríguez MP,Domínguez-Cruz MD,Aguilar-López LB,Borjas-Gutiérrez C,Magaña-Torres MT,González-García JR

    更新日期：2019-01-31 00:00:00

  • Heteromorphic variants of chromosome 9.

    abstract：BACKGROUND:Heterochromatic variants of pericentromere of chromosome 9 are reported and discussed since decades concerning their detailed structure and clinical meaning. However, detailed studies are scarce. Thus, here we provide the largest ever done molecular cytogenetic research based on >300 chromosome 9 heteromorph...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/1755-8166-6-14

    authors： Kosyakova N,Grigorian A,Liehr T,Manvelyan M,Simonyan I,Mkrtchyan H,Aroutiounian R,Polityko AD,Kulpanovich AI,Egorova T,Jaroshevich E,Frolova A,Shorokh N,Naumchik IV,Volleth M,Schreyer I,Nelle H,Stumm M,Wegner RD,Rei

    更新日期：2013-04-01 00:00:00

  • Overexpression of the proneural transcription factor ASCL1 in chronic lymphocytic leukemia with a t(12;14)(q23.2;q32.3).

    abstract：Background:Translocations of the IGH locus on 14q32.3 are present in about 8% of patients with chronic lymphocytic leukemia (CLL) and contribute to leukemogenesis by deregulating the expression of the IGH-partner genes. Identification of these genes and investigation of the downstream effects of their deregulation can ...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-018-0355-7

    authors： Malli T,Rammer M,Haslinger S,Burghofer J,Burgstaller S,Boesmueller HC,Marschon R,Kranewitter W,Erdel M,Deutschbauer S,Webersinke G

    更新日期：2018-01-11 00:00:00

  • Monoallelic and biallelic deletions of 13q14 in a group of CLL/SLL patients investigated by CGH Haematological Cancer and SNP array (8x60K).

    abstract：BACKGROUND:Deletion of 13q14 is the most common cytogenetic change in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and is detected in about 50 % of patients by fluorescence in situ hybridization (FISH), which can reveal presence of del(13)(q14) and mono- or biallelic deletion status without informa...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/s13039-015-0212-x

    authors： Grygalewicz B,Woroniecka R,Rygier J,Borkowska K,Rzepecka I,Łukasik M,Budziłowska A,Rymkiewicz G,Błachnio K,Nowakowska B,Bartnik M,Gos M,Pieńkowska-Grela B

    更新日期：2016-01-06 00:00:00

  • A familial chromosomal complex rearrangement confirms RUNX1T1 as a causative gene for intellectual disability and suggests that 1p22.1p21.3 duplication is likely benign.

    abstract：Background:Complex chromosomal rearrangements are constitutive structural aberrations involving three or more breaks. They can be balanced or unbalanced and result in different outcomes, depending on deletion/duplication of genomic material, gene disruption, or position effects. Case presentation:We report on a patien...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-019-0440-6

    authors： Restaldi F,Alesi V,Aquilani A,Genovese S,Russo S,Coletti V,Pompili D,Falasca R,Dallapiccola B,Capolino R,Luciani M,Novelli A

    更新日期：2019-06-14 00:00:00

  • Identifying novel genetic alterations in pediatric acute lymphoblastic leukemia based on copy number analysis.

    abstract：:Copy number variations (CNVs) analysis may reveal molecular biomarkers and provide information on the pathogenesis of acute lymphoblastic leukemia (ALL). We investigated the gene copy number in childhood ALL by microarray and select three new recurrent CNVs to evaluate by real-time PCR assay: DMBT1, KIAA0125 and PRDM1...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/s13039-020-00491-5

    authors： Batista-Gomes JA,Mello FAR Jr,de Oliveira EHC,de Souza MPC,Wanderley AV,da Costa Pantoja L,Dos Santos NPC,Khayat BCM,Khayat AS

    更新日期：2020-06-26 00:00:00

  • Chromosome 15q13 microduplication in a fetus with cardiac rhabdomyoma: a case report.

    abstract：Background:Copy number variation (CNV) is a complex genomic rearrangement that has been linked to a large number of human diseases. Chromosome 15q13 microduplication is a rare form of CNV, which has been proved to be associated with multiple human disorders; however, the association between chromosome 15q13 microduplic...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-019-0437-1

    authors： Lin CZ,Qi BR,Hu JS,Huang YD,Huang XQ

    更新日期：2019-05-27 00:00:00

  • Short stature and primary ovarian insufficiency possibly due to chromosomal position effect in a balanced X;1 translocation.

    abstract：BACKGROUND:Primary ovarian insufficiency (POI) is defined as a primary ovarian defect characterized by absent menarche (primary amenorrhea), a decrease in the initial primordial follicle number, high follicle-stimulating hormone (FSH) levels and hypoestrogenism. Although the etiology of a majority of POI cases is not y...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-015-0154-3

    authors： Genesio R,Mormile A,Licenziati MR,De Brasi D,Leone G,Balzano S,Izzo A,Bonfiglio F,Conti A,Fioretti G,Lenta S,Poggiano MR,Siani P,Nitsch L

    更新日期：2015-07-15 00:00:00

  • A rare coincidence of different types of driver mutations among uterine leiomyomas (UL).

    abstract：:Mutations of mediator subcomplex 12 (MED12) and of high mobility group protein AT-hook 2 (HMGA2) are driver mutations in uterine leiomyomas (UL) that have not been observed to coexist in one tumor and even rarely coexist in different UL tumors of one patient. Here we describe a patient who underwent hysterectomy becau...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-015-0177-9

    authors： Holzmann C,Markowski DN,Bartnitzke S,Koczan D,Helmke BM,Bullerdiek J

    更新日期：2015-10-14 00:00:00

  • Microdeletions in 9q33.3-q34.11 in five patients with intellectual disability, microcephaly, and seizures of incomplete penetrance: is STXBP1 not the only causative gene?

    abstract：BACKGROUND:Most microdeletions involving chromosome sub-bands 9q33.3-9q34.11 to this point have been detected by analyses focused on STXBP1, a gene known to cause early infantile epileptic encephalopathy 4 and other seizure phenotypes. Loss-of-function mutations of STXBP1 have also been identified in some patients with...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/s13039-015-0178-8

    authors： Ehret JK,Engels H,Cremer K,Becker J,Zimmermann JP,Wohlleber E,Grasshoff U,Rossier E,Bonin M,Mangold E,Bevot A,Schön S,Heilmann-Heimbach S,Dennert N,Mathieu-Dramard M,Lacaze E,Plessis G,de Broca A,Jedraszak G,Röthlis

    更新日期：2015-09-29 00:00:00

  • 7p15 deletion as the cause of hand-foot-genital syndrome: a case report, literature review and proposal of a minimum region for this phenotype.

    abstract：Background:Hand-foot-genital syndrome (HFGS) is a rare condition characterized by congenital malformations in the limbs and genitourinary tract. Generally, this syndrome occurs due to point mutations that cause loss of function of the HOXA13 gene, which is located on 7p15; however, there are some patients with HFGS cau...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-017-0345-1

    authors： Yokoyama E,Smith-Pellegrin DL,Sánchez S,Molina B,Rodríguez A,Juárez R,Lieberman E,Avila S,Castrillo JL,Del Castillo V,Frías S

    更新日期：2017-11-15 00:00:00

  • Jumping translocations of chromosome 1q occurring by a multi-stage process in an acute myeloid leukemia progressed from myelodysplastic syndrome with a TET2 mutation.

    abstract：Background:Jumping translocations (JTs) are rare chromosome rearrangements characterized by re-localization of one donor chromosome to multiple recipient chromosomes. Here, we describe an acute myeloid leukemia (AML) that progressed from myelodysplastic syndrome (MDS) in association with acquisition of 1q JTs. The sequ...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-019-0460-2

    authors： Lee I,Gudipati MA,Waters E,Duong VH,Baer MR,Zou Y

    更新日期：2019-11-19 00:00:00

  • GSTM1 copy number variation in the context of single nucleotide polymorphisms in the human GSTM cluster.

    abstract：BACKGROUND:GSTM1 gene deletion is one of the most known copy number polymorphisms in human genome. It is most likely caused by homologous recombination between the repeats flanking the gene. However, taking into account that the deletion has no crucial effects on human well-being, and the ability of other GSTMs to comp...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/s13039-016-0241-0

    authors： Khrunin AV,Filippova IN,Aliev AM,Tupitsina TV,Slominsky PA,Limborska SA

    更新日期：2016-04-19 00:00:00

  • Deletions in the 17q chromosomal region and their influence on the clonal cytogenetic evolution of recurrent meningiomas.

    abstract：Objective:Meningiomas are among the most frequent intracranial tumors. Although the majority of meningiomas can be cured by surgical resection, up to 20% of the patients develop an aggressive clinical course with tumor recurrence or progressive disease.Cytogenetically, meningiomas frequently harbour a normal karyotype ...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/s13039-019-0434-4

    authors： Hemmer S,Urbschat S,Oertel J,Ketter R

    更新日期：2019-05-24 00:00:00

  • Unexpected structural complexity of supernumerary marker chromosomes characterized by microarray comparative genomic hybridization.

    abstract：BACKGROUND:Supernumerary marker chromosomes (SMCs) are structurally abnormal extra chromosomes that cannot be unambiguously identified by conventional banding techniques. In the past, SMCs have been characterized using a variety of different molecular cytogenetic techniques. Although these techniques can sometimes iden...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/1755-8166-1-7

    authors： Tsuchiya KD,Opheim KE,Hannibal MC,Hing AV,Glass IA,Raff ML,Norwood T,Torchia BA

    更新日期：2008-04-21 00:00:00

  • Frequency of the 7q11.23 inversion polymorphism in transmitting parents of children with Williams syndrome and in the general population does not differ between North America and Europe.

    abstract：: Inversion of the Williams syndrome (WS) region on chromosome 7q11.23 has previously been shown to occur at a higher frequency in the transmitting parents of children with WS than in the general population, suggesting that it predisposes to the WS deletion. Frohnauer et al. recently reported that the frequency of this...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/1755-8166-4-7

    authors： Morris CA,Mervis CB,Osborne LR

    更新日期：2011-02-28 00:00:00

  • 13q deletion syndrome resulting from balanced chromosomal rearrangement in father: the significance of parental karyotyping.

    abstract：Background:Retinoblastoma is a malignancy of the eye in children characterized by biallelic inactivation of the retinoblastoma 1 gene (RB1), located at chromosome 13q14.2. Children with interstitial chromosome 13q deletions that include the RB1 gene show a predisposition to develop retinoblastoma and variable other fea...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-020-00500-7

    authors： Dittner-Moormann S,Reschke M,Biewald E,Kuechler A,Klein B,Timmermann B,Lohmann D,Ketteler P,Kanber D

    更新日期：2020-07-23 00:00:00

  • Hyperdiploidy associated with T315I mutation in BCR-ABL kinase domain in an accelerated phase-chronic myeloid leukemia case.

    abstract：BACKGROUND:Chronic myeloid leukemia (CML) is genetically characterized by the occurrence of a reciprocal translocation t(9;22)(q34;q11), resulting in a BCR/ABL gene fusion on the derivative chromosome 22, i.e. the Philadelphia (Ph) chromosome. During CML progression 60-80% of the cases acquire additional genetic change...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-014-0089-0

    authors： Al-Achkar W,Moassass F,Ikhtiar A,Liehr T,Othman MA,Wafa A

    更新日期：2014-11-29 00:00:00

  • Identification of small segmental translocations in patients with repeated implantation failure and recurrent miscarriage using next generation sequencing after in vitro fertilization/intracytoplasmic sperm injection.

    abstract：BACKGROUND:To develop a novel preimplantation genetic screening (PGS) test using next generation sequencing(NGS) as a alternative to current array comparative genomic hybridization (array CGH) method for detection of small segmental translocations in two patients with repeated implantation failure (RIF) and recurrent m...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-015-0207-7

    authors： Ou J,Wang W,Feng T,Liao L,Meng Q,Zou Q,Ding J,Zheng A,Duan C,Li P,Liu Q,Lin C,Li H

    更新日期：2015-12-30 00:00:00

  • Position of chromosomes 18, 19, 21 and 22 in 3D-preserved interphase nuclei of human and gorilla and white hand gibbon.

    abstract：BACKGROUND:Even though comparative nuclear architecture studies in hominoids are sparse, nuclear chromosome architecture was shown to be conserved during hominoid evolution. Thus, it is suspected that yet unknown biological mechanisms must underlie this observation. RESULTS:Here for the first time a combination of mul...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/1755-8166-1-9

    authors： Manvelyan M,Hunstig F,Mrasek K,Bhatt S,Pellestor F,Weise A,Liehr T

    更新日期：2008-04-29 00:00:00

  • Prolonged exposure to acid and bile induces chromosome abnormalities that precede malignant transformation of benign Barrett's epithelium.

    abstract：UNLABELLED:Barrett's esophagus (BE) is an asymptomatic, pre-malignant condition of the esophagus that can progress to esophageal adenocarcinoma (EAC). BE arises typically in individuals with long-standing gastroesophageal reflux disease (GERD). The neoplastic progression of BE has been extensively studied histologicall...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/1755-8166-5-43

    authors： Bajpai M,Aviv H,Das KM

    更新日期：2012-11-29 00:00:00

  • Automated detection of residual cells after sex-mismatched stem-cell transplantation - evidence for presence of disease-marker negative residual cells.

    abstract：BACKGROUND:A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.Cells of 58 patients were characterized as disease-associated due to presence of a ...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/1755-8166-2-12

    authors： Erlecke J,Hartmann I,Hoffmann M,Kroll T,Starke H,Heller A,Gloria A,Sayer HG,Johannes T,Claussen U,Liehr T,Loncarevic IF

    更新日期：2009-05-29 00:00:00

  • Early detection of Angelman syndrome resulting from de novo paternal isodisomic 15q UPD and review of comparable cases.

    abstract：BACKGROUND:Angelman syndrome is a rare neurogenetic disorder that results in intellectual and developmental disturbances, seizures, jerky movements and frequent smiling. Angelman syndrome is caused by two genetic disturbances: either genes on the maternally inherited chromosome 15 are deleted or inactivated or two pate...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/1755-8166-6-35

    authors： Horváth E,Horváth Z,Isaszegi D,Gergev G,Nagy N,Szabó J,Sztriha L,Széll M,Endreffy E

    更新日期：2013-09-08 00:00:00

  • Maternal uniparental isodisomy for chromosome 6 discovered by paternity testing: a case report.

    abstract：Background:Uniparental disomy (UPD) is a rare condition in which a child inherits both copies of a chromosome or chromosome segment from one parent. Medical consequences of UPD may include abnormal imprinting, unmasking of genetic disease, and somatic mosaicism; alternatively, the condition may be clinically silent. We...

    journal_title：Molecular cytogenetics

    pub_type：

    doi：10.1186/s13039-018-0411-3

    authors： Kerr ER,Stuhlmiller GM,Maha GC,Ladd MA,Mikhail FM,Koester RP,Hurst ACE

    更新日期：2018-12-20 00:00:00

  • Characterization of a prenatally assessed de novo supernumerary minute ring chromosome 20 in a phenotypically normal male.

    abstract：BACKGROUND:The heterogeneous group of small supernumerary marker chromosomes (sSMCs) presents serious counseling problems, especially if they are present de novo and diagnosed prenatally. The incidence has been estimated at 1 in 1000 prenatal samples. We present a case of mosaic sSMC diagnosed prenatally after amniocen...

    journal_title：Molecular cytogenetics

    pub_type： 杂志文章

    doi：10.1186/1755-8166-2-1

    authors： Kitsiou-Tzeli S,Manolakos E,Lagou M,Kontodiou M,Kosyakova N,Ewers E,Weise A,Garas A,Orru S,Liehr T,Metaxotou A

    更新日期：2009-01-07 00:00:00