Abstract:
OBJECTIVE:The objective of this study is to examine the efficacy of erlotinib and gefitinib with respect to epidermal growth factor (EGF) and cetuximab response in head and neck cancer cell lines. MATERIALS AND METHODS:Five human head and neck carcinoma cell lines were treated with EGF, cetuximab, erlotinib, and gefitinib, and the effects were measured with a crystal violet assay. The efficacies of cetuximab, erlotinib, and gefitinib in clinically relevant concentrations were statistically analyzed. The expression of the epidermal growth factor receptor (EGFR) and phosphorylation patterns were detected with fluorescence-activated cell sorting (FACS) analysis and western blot analysis. The endogenous production of EGF by the cells was detected with an enzyme-linked immunosorbent assay. Finally, EGFR, KRAS, BRAF, and PI3K mutation analyses were performed. RESULTS:All of the cell lines had a poor or no response to EGF but exhibited distinct EGFR phosphorylation and EGFR expression. Compared to cetuximab, erlotinib and gefitinib demonstrated a greater impact on the majority of the cell lines. The only cell line that showed a concentration-dependent behavior toward EGF and strong EGFR phosphorylation was entirely resistant to cetuximab, erlotinib, and gefitinib. The production of EGF in all cell lines was very low. Mutational analysis of all cell lines revealed wild-type EGFR, KRAS, BRAF, and PI3K. CONCLUSIONS:The prediction of anti-EGFR treatment cannot be based on responsiveness to EGF or EGFR activation. CLINICAL RELEVANCE:Erlotinib and gefitinib show good response in EGF-independent cell lines and might be useful drugs in tumors that are less responsive to cetuximab.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Hartmann S,Neckel N,Seher A,Mutzbauer G,Brands RC,Linz C,Kübler AC,Müller-Richter UDdoi
10.1007/s00784-015-1566-5subject
Has Abstractpub_date
2016-05-01 00:00:00pages
759-69issue
4eissn
1432-6981issn
1436-3771pii
10.1007/s00784-015-1566-5journal_volume
20pub_type
杂志文章abstract::To present the safety profile, the early healing phase and the clinical outcomes at 24 weeks following treatment of human intrabony defects with open flap debridement (OFD) alone or with OFD and rhGDF-5 adsorbed onto a particulate β-tricalcium phosphate (β-TCP) carrier. Twenty chronic periodontitis patients, each with...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-011-0610-3
更新日期:2012-08-01 00:00:00
abstract::This study evaluated the colour parameters of composite and ceramic shade guides determined using a colorimeter and digital imaging method with illuminants at different colour temperatures. Two different resin composite shade guides, namely Charisma (Heraeus Kulzer) and Premise (Kerr Corporation), and two different ce...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0329-6
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was the qualitative and quantitative evaluation of osseous graft consolidation using allogeneic bone blocks for vertical bone augmentation in an animal model. MATERIAL AND METHODS:Standardised allogeneic and autologous bone blocks were fixed on the frontal skull of 20 adult fema...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03151-0
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to evaluate the biomechanical behavior of the interface formed between bone and implants with machined surfaces (MS) and those modified by Al2O3 sandblasting and acid etching (SBAS). MATERIALS AND METHODS:Before surgery, topographic characterization was performed by SEM-EDX and...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02872-6
更新日期:2019-12-01 00:00:00
abstract::The aim of this randomized double-blinded preliminary study was to evaluate the clinical and microbiological long-term effects of an essential-oil-containing mouth rinse as the active agent utilized in the "one-stage full-mouth disinfection protocol." Probing pocket depth and plaque and gingival indices were evaluated...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-008-0219-3
更新日期:2009-06-01 00:00:00
abstract:OBJECTIVE:The purpose of this double-blind, randomised trial was to compare the clinical performance of a hybrid composite (Clearfil AP-X, Kuraray, Tokyo) and a nanocomposite (Filtek Z350, 3M ESPE, St. Paul, MN) over a period of 2 years in non-carious class V lesions using a modified US Public Health Service (USPHS) sy...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-012-0780-7
更新日期:2013-04-01 00:00:00
abstract::Aim of this investigation was to evaluate the validity and reproducibility of the universal visual scoring systems (UniViSS) on occlusal surfaces in vitro. The validity study included 65 third molars. Following to reach a UniViSS consensus diagnosis for each surface, all teeth were histologically prepared and evaluate...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-010-0390-1
更新日期:2011-04-01 00:00:00
abstract::The hypotheses tested were: survival rate of fully and partially retained glass-carbomer sealants is higher than those of high-viscosity glass-ionomer, with and without energy supplied, and that of resin composite; survival rate of fully and partially retained sealants of high-viscosity glass-ionomer with energy suppl...
journal_title:Clinical oral investigations
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00784-011-0633-9
更新日期:2012-10-01 00:00:00
abstract::The aim of the current study was to evaluate the prevalence of psychopathological symptoms in patients who self-reported different forms of bruxism by means of clinical and anamnestic diagnostic criteria. Eighty-five participants were divided into four groups as sleep bruxers (12), awake bruxers (24), sleep-awake brux...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-010-0492-9
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVE:Noninvasive optical methods such as photoplethysmography, established for blood pulse detection in organs, have been proposed for vitality testing of human dental pulp. However, no information is available on the mechanism of action in a closed pulp chamber and on the impairing influence of other than pulpal ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1115-z
更新日期:2014-01-01 00:00:00
abstract::Dear Professor Dr. Matthias Hannig. Editor-in-Chief Clinical Oral Investigations. Following the publication of our paper by Calvo Guirado et al, 2015 [1] in Clinical Oral Investigations, it came to light that a certain part of the text at the materials and methods and results sections were similar. ...
journal_title:Clinical oral investigations
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00784-018-2763-9
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:To assess the association between periodontal disease and Helicobacter pylori (H. pylori) infection in oral cavity. MATERIALS AND METHODS:We searched PubMed, Embase, Web of Science, Cochrane library, Gray literature, and clinicaltrials.gov for eligible studies up to September 25, 2019. Odds ratios (ORs) and...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-020-03330-4
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of daily exposure to a denture cleanser on a multispecies biofilm. MATERIALS AND METHODS:Multispecies biofilms (five bacteria and Candida albicans) were developed for 64.5 h on acrylic resin specimens and randomized into control and experimental groups. In the...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-014-1210-9
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVE:The aim of this in vitro study was to compare fluorescence-aided caries excavation with conventional excavation based on the Martens and Vickers hardness of dentin at the cavity floor after caries removal. MATERIALS AND METHODS:In total, 20 extracted human teeth with dentin caries were bisected through the l...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0999-y
更新日期:2014-01-01 00:00:00
abstract::This study evaluated the surface roughness change and wear provided by different dentifrices on root dentine previously exposed to erosive challenges. According to a randomized complete block design, 150 slabs of bovine root dentine (6 x 3 x 2 mm) were ground flat and polished. In an area of 4 x 3 mm on the dentine su...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-004-0262-7
更新日期:2004-09-01 00:00:00
abstract::The main objective of this study was to identify the risk factors for the incidence of visible caries experience in a cohort of preschool children living in Flanders. Data were collected from 1,057 children; validated questionnaires on oral health-related behaviour were completed by parents at birth (2003-2004), at ag...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-011-0579-y
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVES:The objective of the study was to compare the retreatability of three calcium silicate-containing sealers (BioRoot RCS, MTA Fillapex, Endo C.P.M.) and an epoxy resin-based sealer (AH Plus) with different root canal instruments (Hedström files, Reciproc R40, Mtwo retreatment file R 25/.05 + Mtwo 40/.06, and F...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2156-5
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVES:Investigate the biochemistry of in vivo healthy oral tissues through Raman spectroscopy. We aimed to characterize the biochemical features of healthy condition in oral subsites (buccal mucosa, lip, tongue, and gingiva) of healthy subjects. More specifically, we investigated Raman spectral characteristics and...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2714-5
更新日期:2019-07-01 00:00:00
abstract::The purpose of this study was to determine the risk of microbiological transmission into the dental laboratory via impressions and dental stone models. Metal master models were contaminated with Bacillus subtilis and Streptococcus sanguis. Impressions in alginate, polyvinylsiloxane, and polyether were used, and models...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-002-0174-3
更新日期:2002-09-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to evaluate the association between salivary and plaque arginine levels/ADS activities with dental caries. MATERIALS AND METHODS:A systematic search was performed as per PRISMA statement using PubMed, Scopus, Cochrane Library, and Web of Science. Published studies that invest...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00784-017-2322-9
更新日期:2018-03-01 00:00:00
abstract::This study compared shear bond strengths of six self-adhesive resin cements to zirconium oxide ceramic with and without air-particle abrasion. One hundred twenty zirconia samples were air-abraded (group SB; n = 60) or left untreated (group NO). Composite cylinders were bonded to the zirconia samples with either BisCem...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0278-0
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate differences between the virtually planned and clinically achieved implant positions in completely template-guided implantations as a function of the type of edentulous space, the residual natural dentition, and the surgical implementation. MATERIALS AND METHODS:Fifty-...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2339-8
更新日期:2018-07-01 00:00:00
abstract::Squamous cell carcinoma (SCC) of the oral cavity is an extremely invasive tumour of stratified squamous epithelium that spreads throughout degradation of the basement membrane (BM) and extra-cellular matrix. Oral verrucous carcinoma (VC) is a rare low-grade variant of oral SCC that penetrates into the subepithelial co...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0296-y
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate repair after endodontic surgery using two- and tridimensional imaging methods. MATERIALS AND METHODS:Periapical radiographs and cone beam computed tomography (CBCT) were performed before the surgeries and after 48 h (baseline), 4 months, and 8 months. The area (square mi...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1225-2
更新日期:2015-01-01 00:00:00
abstract::Resin-bonded fixed partial dentures (RBFPD) are used as a minimal invasive, tooth-preventing alternative for replacing anterior teeth. Zirconia cantilever restorations were supposed to show sufficient strength for a clinical application. The aim of this investigation was to determine the fracture characteristics of ca...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0254-8
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVE:The study aimed to evaluate the effect of internal silver coating as a countermeasure to crestal bone loss around implants with or without multiple abutment disconnections/reconnections. MATERIALS AND METHODS:Following tooth extraction, 48 implants with connected healing abutments (24 implants internally coa...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03416-z
更新日期:2021-02-01 00:00:00
abstract:OBJECTIVES:The aims of this study are to evaluate the dentofacial morphology of patients with rheumatoid arthritis (RA) and to compare the morphological data with those of healthy age- and sex-matched control subjects. METHODS:Twenty-seven RA patients (mean age, 45.77 ± 8.64 years) and 25 healthy subjects (mean age, 4...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-012-0861-7
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVES:This case series compared the clinical survival of tooth-implant-supported (TI-S) and tooth-supported (T-S) three-unit fixed dental prostheses (FDPs) with zirconia frameworks and described the incidence of biological and technical complications. MATERIALS AND METHODS:Forty-four patients received 27 TI-S FDP...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1594-1
更新日期:2016-06-01 00:00:00
abstract::The aim of this study was to determine the prevalence of dental erosion in young Icelandic adults (19-22 years old) and patients with gastroesophageal reflux disease (GERD), in relation to their soft drink consumption and gastroesophageal reflux. Eighty subjects (40 males and 40 females), comprising 57 young adults (m...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-003-0252-1
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVES:To analyze the potential of human dental pulp stem cells (hDPSCs) for maintaining their undifferentiated status and osteogenic differentiation capacity when arranged in cell sheets (CSs) for future application in bone replacement. MATERIALS AND METHODS:CSs were formed after being induced for 10-15 days by c...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2630-8
更新日期:2019-06-01 00:00:00