Endogenous siRNAs and piRNAs derived from transposable elements and genes in the malaria vector mosquito Anopheles gambiae.

Abstract:

BACKGROUND:The siRNA and piRNA pathways have been shown in insects to be essential for regulation of gene expression and defence against exogenous and endogenous genetic elements (viruses and transposable elements). The vast majority of endogenous small RNAs produced by the siRNA and piRNA pathways originate from repetitive or transposable elements (TE). In D. melanogaster, TE-derived endogenous siRNAs and piRNAs are involved in genome surveillance and maintenance of genome integrity. In the medically relevant malaria mosquito Anopheles gambiae TEs constitute 12-16% of the genome size. Genetic variations induced by TE activities are known to shape the genome landscape and to alter the fitness in An. gambiae. RESULTS:Here, using bioinformatics approaches we analyzed the small RNA data sets from 6 libraries formally reported in a previous study and examined the expression of the mixed germline/somatic siRNAs and piRNAs produced in adult An. gambiae females. We characterized a large population of TE-derived endogenous siRNAs and piRNAs, which constitutes 56-60% of the total siRNA and piRNA reads in the analysed libraries. Moreover, we identified a number of protein coding genes producing gene-specific siRNAs and piRNAs that were generally expressed at much lower levels than the TE-associated small RNAs. Detailed sequence analysis revealed that An. gambiae piRNAs were produced by both "ping-pong" dependent (TE-associated piRNAs) and independent mechanisms (genic piRNAs). Similarly to D. melanogaster, more than 90% of the detected piRNAs were produced from TE-associated clusters in An. gambiae. We also found that biotic stress as blood feeding and infection with Plasmodium parasite, the etiological agent of malaria, modulated the expression levels of the endogenous siRNAs and piRNAs in An. gambiae. CONCLUSIONS:We identified a large and diverse set of the endogenously derived siRNAs and piRNAs that share common and distinct aspects of small RNA expression across insect species, and inferred their impact on TE and gene activity in An. gambiae. The TE-specific small RNAs produced by both the siRNA and piRNA pathways represent an important aspect of genome stability and genetic variation, which might have a strong impact on the evolution of the genome and vector competence in the malaria mosquitoes.

journal_name

BMC Genomics

journal_title

BMC genomics

authors

Biryukova I,Ye T

doi

10.1186/s12864-015-1436-1

subject

Has Abstract

pub_date

2015-04-10 00:00:00

pages

278

issn

1471-2164

pii

10.1186/s12864-015-1436-1

journal_volume

16

pub_type

杂志文章
  • The action of selection on codon bias in the human genome is related to frequency, complexity, and chronology of amino acids.

    abstract:BACKGROUND:The question of whether synonymous codon choice is affected by cellular tRNA abundance has been positively answered in many organisms. In some recent works, concerning the human genome, this relation has been studied, but no conclusive answers have been found. In the human genome, the variation in base compo...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-7-67

    authors: Kotlar D,Lavner Y

    更新日期:2006-04-03 00:00:00

  • Fine mapping and RNA-Seq unravels candidate genes for a major QTL controlling multiple fiber quality traits at the T1 region in upland cotton.

    abstract:BACKGROUND:Improving fiber quality is a major challenge in cotton breeding, since the molecular basis of fiber quality traits is poorly understood. Fine mapping and candidate gene prediction of quantitative trait loci (QTL) controlling cotton fiber quality traits can help to elucidate the molecular basis of fiber quali...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2605-6

    authors: Liu D,Zhang J,Liu X,Wang W,Liu D,Teng Z,Fang X,Tan Z,Tang S,Yang J,Zhong J,Zhang Z

    更新日期:2016-04-19 00:00:00

  • Phylogenetic patterns of emergence of new genes support a model of frequent de novo evolution.

    abstract:BACKGROUND:New gene emergence is so far assumed to be mostly driven by duplication and divergence of existing genes. The possibility that entirely new genes could emerge out of the non-coding genomic background was long thought to be almost negligible. With the increasing availability of fully sequenced genomes across ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-14-117

    authors: Neme R,Tautz D

    更新日期:2013-02-21 00:00:00

  • Sequence differences in the seed dormancy gene Qsd1 among various wheat genomes.

    abstract:BACKGROUND:Pre-harvest sprouting frequently occurs in Triticum aestivum (wheat) and Hordeum vulgare (barley) at the end of the maturity period due to high rainfall, particularly in Asian monsoon areas. Seed dormancy is a major mechanism preventing pre-harvest sprouting in these crops. RESULTS:We identified orthologous...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-3880-6

    authors: Onishi K,Yamane M,Yamaji N,Tokui M,Kanamori H,Wu J,Komatsuda T,Sato K

    更新日期:2017-06-29 00:00:00

  • Gene expression profiling in peanut using high density oligonucleotide microarrays.

    abstract:BACKGROUND:Transcriptome expression analysis in peanut to date has been limited to a relatively small set of genes and only recently has a significant number of ESTs been released into the public domain. Utilization of these ESTs for oligonucleotide microarrays provides a means to investigate large-scale transcript res...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-265

    authors: Payton P,Kottapalli KR,Rowland D,Faircloth W,Guo B,Burow M,Puppala N,Gallo M

    更新日期:2009-06-12 00:00:00

  • Transcriptome sequencing of a keystone aquatic herbivore yields insights on the temperature-dependent metabolism of essential lipids.

    abstract:BACKGROUND:Nutritional quality of phytoplankton is a major determinant of the trophic transfer efficiency at the plant-herbivore interface in freshwater food webs. In particular, the phytoplankton's content of the essential polyunsaturated omega-3 fatty acid eicosapentaenoic acid (EPA) has been repeatedly shown to limi...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-6268-y

    authors: Windisch HS,Fink P

    更新日期:2019-11-21 00:00:00

  • Differential control of Zap1-regulated genes in response to zinc deficiency in Saccharomyces cerevisiae.

    abstract:BACKGROUND:The Zap1 transcription factor is a central player in the response of yeast to changes in zinc status. We previously used transcriptome profiling with DNA microarrays to identify 46 potential Zap1 target genes in the yeast genome. In this new study, we used complementary methods to identify additional Zap1 ta...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-9-370

    authors: Wu CY,Bird AJ,Chung LM,Newton MA,Winge DR,Eide DJ

    更新日期:2008-08-01 00:00:00

  • A systematic evaluation of expression of HERV-W elements; influence of genomic context, viral structure and orientation.

    abstract:BACKGROUND:One member of the W family of human endogenous retroviruses (HERV) appears to have been functionally adopted by the human host. Nevertheless, a highly diversified and regulated transcription from a range of HERV-W elements has been observed in human tissues and cells. Aberrant expression of members of this f...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-22

    authors: Li F,Nellåker C,Yolken RH,Karlsson H

    更新日期:2011-01-12 00:00:00

  • Comparative transcript profiling of the fertile and sterile flower buds of pol CMS in B. napus.

    abstract:BACKGROUND:The Polima (pol) system of cytoplasmic male sterility (CMS) and its fertility restoration gene Rfp have been used in hybrid breeding in Brassica napus, which has greatly improved the yield of rapeseed. However, the mechanism of the male sterility transition in pol CMS remains to be determined. RESULTS:To in...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-258

    authors: An H,Yang Z,Yi B,Wen J,Shen J,Tu J,Ma C,Fu T

    更新日期:2014-04-03 00:00:00

  • RNA profiles of rat olfactory epithelia: individual and age related variations.

    abstract:BACKGROUND:Mammalian genomes contain a large number (approximately 1000) of olfactory receptor (OR) genes, many of which (20 to 50%) are pseudogenes. OR gene transcription is not restricted to the olfactory epithelium, but is found in numerous tissues. Using microarray hybridization and RTqPCR, we analyzed the mRNA pro...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-572

    authors: Rimbault M,Robin S,Vaysse A,Galibert F

    更新日期:2009-12-02 00:00:00

  • Transcriptome analysis revealed the drought-responsive genes in Tibetan hulless barley.

    abstract:BACKGROUND:Hulless barley, also called naked barley, is an important cereal crop worldwide, serving as a healthy food both for human consumption and animal feed. Nevertheless, it often suffered from drought stress during its growth and development, resulting in a drastic reduction in barley yields. Therefore, study on ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2685-3

    authors: Zeng X,Bai L,Wei Z,Yuan H,Wang Y,Xu Q,Tang Y,Nyima T

    更新日期:2016-05-20 00:00:00

  • BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.).

    abstract:BACKGROUND:Although second generation sequencing (2GS) technologies allow re-sequencing of previously gold-standard-sequenced genomes, whole genome shotgun sequencing and de novo assembly of large and complex eukaryotic genomes is still difficult. Availability of a genome-wide physical map is therefore still a prerequi...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-247

    authors: Schulte D,Ariyadasa R,Shi B,Fleury D,Saski C,Atkins M,deJong P,Wu CC,Graner A,Langridge P,Stein N

    更新日期:2011-05-19 00:00:00

  • Characterization of the lipoxygenase (LOX) gene family in the Chinese white pear (Pyrus bretschneideri) and comparison with other members of the Rosaceae.

    abstract:BACKGROUND:Lipoxygenases (LOXs), a type of non-haem iron-containing dioxygenase, are ubiquitous enzymes in plants and participate in the formation of fruit aroma which is a very important aspect of fruit quality. Amongst the various aroma volatiles, saturated and unsaturated alcohols and aldehydes provide the character...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-444

    authors: Li M,Li L,Dunwell JM,Qiao X,Liu X,Zhang S

    更新日期:2014-06-07 00:00:00

  • Leaf transcriptome analysis of a subtropical evergreen broadleaf plant, wild oil-tea camellia (Camellia oleifera), revealing candidate genes for cold acclimation.

    abstract:BACKGROUND:Cold tolerance is a key determinant of the geographical distribution range of a plant species and crop production. Cold acclimation can enhance freezing-tolerance of plant species through a period of exposure to low nonfreezing temperatures. As a subtropical evergreen broadleaf plant, oil-tea camellia demons...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-3570-4

    authors: Chen J,Yang X,Huang X,Duan S,Long C,Chen J,Rong J

    更新日期:2017-02-28 00:00:00

  • A multispecies comparison of the metazoan 3'-processing downstream elements and the CstF-64 RNA recognition motif.

    abstract:BACKGROUND:The Cleavage Stimulation Factor (CstF) is a required protein complex for eukaryotic mRNA 3'-processing. CstF interacts with 3'-processing downstream elements (DSEs) through its 64-kDa subunit, CstF-64; however, the exact nature of this interaction has remained unclear. We used EST-to-genome alignments to ide...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-7-55

    authors: Salisbury J,Hutchison KW,Graber JH

    更新日期:2006-03-16 00:00:00

  • Dissection of complicate genetic architecture and breeding perspective of cottonseed traits by genome-wide association study.

    abstract:BACKGROUND:Cottonseed is one of the most important raw materials for plant protein, oil and alternative biofuel for diesel engines. Understanding the complex genetic basis of cottonseed traits is requisite for achieving efficient genetic improvement of the traits. However, it is not yet clear about their genetic archit...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4837-0

    authors: Du X,Liu S,Sun J,Zhang G,Jia Y,Pan Z,Xiang H,He S,Xia Q,Xiao S,Shi W,Quan Z,Liu J,Ma J,Pang B,Wang L,Sun G,Gong W,Jenkins JN,Lou X,Zhu J,Xu H

    更新日期:2018-06-13 00:00:00

  • Transcriptomic time-series analysis of early development in olive from germinated embryos to juvenile tree.

    abstract:BACKGROUND:Despite its relevance, almost no studies account for the genetic control in the early stages of tree development, i.e. from germination on. This study seeks to make a quite complete transcriptome for olive development and to elucidate the dynamic regulation of the transcriptomic response during the early-juv...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5232-6

    authors: Jiménez-Ruiz J,de la O Leyva-Pérez M,Vidoy-Mercado I,Barceló A,Luque F

    更新日期:2018-11-19 00:00:00

  • Transcriptome analysis reveals key roles of AtLBR-2 in LPS-induced defense responses in plants.

    abstract:BACKGROUND:Lipopolysaccharide (LPS) from Gram-negative bacteria cause innate immune responses in animals and plants. The molecules involved in LPS signaling in animals are well studied, whereas those in plants are not yet as well documented. Recently, we identified Arabidopsis AtLBR-2, which binds to LPS from Pseudomon...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-4372-4

    authors: Iizasa S,Iizasa E,Watanabe K,Nagano Y

    更新日期:2017-12-29 00:00:00

  • Exclusivity offers a sound yet practical species criterion for bacteria despite abundant gene flow.

    abstract:BACKGROUND:The question of whether bacterial species objectively exist has long divided microbiologists. A major source of contention stems from the fact that bacteria regularly engage in horizontal gene transfer (HGT), making it difficult to ascertain relatedness and draw boundaries between taxa. A natural way to defi...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5099-6

    authors: Wright ES,Baum DA

    更新日期:2018-10-03 00:00:00

  • Different transcriptional response between susceptible and resistant common carp (Cyprinus carpio) fish hints on the mechanism of CyHV-3 disease resistance.

    abstract:BACKGROUND:Infectious disease outbreaks form major setbacks to aquaculture production and to further development of this important sector. Cyprinid herpes virus-3 (CyHV-3) is a dsDNA virus widely hampering production of common carp (Cyprinus carpio), one of the most farmed fish species worldwide. Genetically disease re...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-6391-9

    authors: Tadmor-Levi R,Doron-Faigenboim A,Marcos-Hadad E,Petit J,Hulata G,Forlenza M,Wiegertjes GF,David L

    更新日期:2019-12-26 00:00:00

  • Highly expressed captured genes and cross-kingdom domains present in Helitrons create novel diversity in Pleurotus ostreatus and other fungi.

    abstract:BACKGROUND:Helitrons are class-II eukaryotic transposons that transpose via a rolling circle mechanism. Due to their ability to capture and mobilize gene fragments, they play an important role in the evolution of their host genomes. We have used a bioinformatics approach for the identification of helitrons in two Pleur...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-1071

    authors: Castanera R,Pérez G,López L,Sancho R,Santoyo F,Alfaro M,Gabaldón T,Pisabarro AG,Oguiza JA,Ramírez L

    更新日期:2014-12-05 00:00:00

  • Complete chloroplast genome sequence of Betula platyphylla: gene organization, RNA editing, and comparative and phylogenetic analyses.

    abstract:BACKGROUND:Betula platyphylla is a common tree species in northern China that has high economic and medicinal value. Our laboratory has been devoted to genome research on B. platyphylla for approximately 10 years. As primary organelle genomes, the complete genome sequences of chloroplasts are important to study the div...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5346-x

    authors: Wang S,Yang C,Zhao X,Chen S,Qu GZ

    更新日期:2018-12-20 00:00:00

  • Differences in transcription between free-living and CO2-activated third-stage larvae of Haemonchus contortus.

    abstract:BACKGROUND:The disease caused by Haemonchus contortus, a blood-feeding nematode of small ruminants, is of major economic importance worldwide. The infective third-stage larva (L3) of this gastric nematode is enclosed in a cuticle (sheath) and, once ingested with herbage by the host, undergoes an exsheathment process th...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-266

    authors: Cantacessi C,Campbell BE,Young ND,Jex AR,Hall RS,Presidente PJ,Zawadzki JL,Zhong W,Aleman-Meza B,Loukas A,Sternberg PW,Gasser RB

    更新日期:2010-04-27 00:00:00

  • Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages.

    abstract:BACKGROUND:Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3, a kind of ω-3 FAs)....

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-716

    authors: Wang X,Xu R,Wang R,Liu A

    更新日期:2012-12-20 00:00:00

  • Inference of gene interaction networks using conserved subsequential patterns from multiple time course gene expression datasets.

    abstract:MOTIVATION:Deciphering gene interaction networks (GINs) from time-course gene expression (TCGx) data is highly valuable to understand gene behaviors (e.g., activation, inhibition, time-lagged causality) at the system level. Existing methods usually use a global or local proximity measure to infer GINs from a single dat...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-16-S12-S4

    authors: Liu Q,Song R,Li J

    更新日期:2015-01-01 00:00:00

  • Characteristics and clustering of human ribosomal protein genes.

    abstract:BACKGROUND:The ribosome is a central player in the translation system, which in mammals consists of four RNA species and 79 ribosomal proteins (RPs). The control mechanisms of gene expression and the functions of RPs are believed to be identical. Most RP genes have common promoters and were therefore assumed to have a ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-7-37

    authors: Ishii K,Washio T,Uechi T,Yoshihama M,Kenmochi N,Tomita M

    更新日期:2006-02-28 00:00:00

  • Mitochondrial dysregulation and oxidative stress in patients with chronic kidney disease.

    abstract:BACKGROUND:Chronic renal disease (CKD) is characterized by complex changes in cell metabolism leading to an increased production of oxygen radicals, that, in turn has been suggested to play a key role in numerous clinical complications of this pathological condition. Several reports have focused on the identification o...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-388

    authors: Granata S,Zaza G,Simone S,Villani G,Latorre D,Pontrelli P,Carella M,Schena FP,Grandaliano G,Pertosa G

    更新日期:2009-08-21 00:00:00

  • Structure and evolution of barley powdery mildew effector candidates.

    abstract:BACKGROUND:Protein effectors of pathogenicity are instrumental in modulating host immunity and disease resistance. The powdery mildew pathogen of grasses Blumeria graminis causes one of the most important diseases of cereal crops. B. graminis is an obligate biotrophic pathogen and as such has an absolute requirement to...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-694

    authors: Pedersen C,Ver Loren van Themaat E,McGuffin LJ,Abbott JC,Burgis TA,Barton G,Bindschedler LV,Lu X,Maekawa T,Wessling R,Cramer R,Thordal-Christensen H,Panstruga R,Spanu PD

    更新日期:2012-12-11 00:00:00

  • Genome-wide study on genetic diversity and phylogeny of five species in the genus Cervus.

    abstract:BACKGROUND:Previous investigations of phylogeny in Cervus recovered many clades without whole genomic support. METHODS:In this study, the genetic diversity and phylogeny of 5 species (21 subspecies/populations from C. unicolor, C. albirostris, C. nippon, C. elaphus and C. eldii) in the genus Cervus were analyzed using...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-5785-z

    authors: Hu P,Shao Y,Xu J,Wang T,Li Y,Liu H,Rong M,Su W,Chen B,Cui S,Cui X,Yang F,Tamate H,Xing X

    更新日期:2019-05-17 00:00:00

  • QTLs associated with dry matter intake, metabolic mid-test weight, growth and feed efficiency have little overlap across 4 beef cattle studies.

    abstract:BACKGROUND:The identification of genetic markers associated with complex traits that are expensive to record such as feed intake or feed efficiency would allow these traits to be included in selection programs. To identify large-effect QTL, we performed a series of genome-wide association studies and functional analyse...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-1004

    authors: Saatchi M,Beever JE,Decker JE,Faulkner DB,Freetly HC,Hansen SL,Yampara-Iquise H,Johnson KA,Kachman SD,Kerley MS,Kim J,Loy DD,Marques E,Neibergs HL,Pollak EJ,Schnabel RD,Seabury CM,Shike DW,Snelling WM,Spangler ML,

    更新日期:2014-11-20 00:00:00