Abstract：
:The ability to selectively control the differentiation of neural stem cells (NSCs) into cholinergic neurons in vivo would be an important step toward cell replacement therapy. First, green fluorescent protein (GFP)-NSCs were induced to differentiate into cholinergic neuron-like cells (CNLs) with retinoic acid (RA) pre-induction followed by nerve growth factor (NGF) induction. Then, these CNLs were transplanted into bilateral hippocampus of APP/PS1 transgenic mice. Behavioral parameters showed by Morris water maze (MWM) tests and the percentages of GFP-labeled cholinergic neurons of CNL transplanted mice were compared with those of controls. Brain levels of choline acetyltransferase (ChAT) mRNA and proteins were analyzed by quantitative real-time PCR and Western blotting, ChAT activity and acetylcholine (ACh) concentration were also evaluated by ChAT activity and ACh concentration assay kits. Immunofluorescence analysis showed that 80.3±1.5% NSCs differentiated into CNLs after RA pre-induction followed by NGF induction in vitro. Three months after transplantation, 82.4±6.3% CNLs differentiated into cholinergic neurons in vivo. APP/PS1 mice transplanted with CNLs showed a significant improvement in learning and memory ability compared with control groups at different time points. Furthermore, CNLs transplantation dramatically increased in the expressions of ChAT mRNA and protein, as well ChAT activity and ACh concentration in APP/PS1 mice. Our findings support the prospect of using NSC-derived CNLs in developing therapies for Alzheimer's disease (AD).
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Gu G,Zhang W,Li M,Ni J,Wang Pdoi
10.1016/j.neuroscience.2015.01.073subject
Has Abstractpub_date
2015-04-16 00:00:00pages
81-92eissn
0306-4522issn
1873-7544pii
S0306-4522(15)00141-4journal_volume
291pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:We used whole-cell patch recording to study 102 local interneurons in the rat dorsal lateral geniculate nucleus in vitro. Input impedance with this technique (607.0+/-222.4 MOhm) was far larger than that measured with sharp electrode techniques, suggesting that interneurons may be more electrotonically compact than pr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00665-4
更新日期：1999-01-01 00:00:00
abstract：:Free radicals have been implicated in a number of pathological conditions. To evaluate the neurophysiological consequences of free radical exposure, slices of hippocampus isolated from guinea-pigs were exposed to hydrogen peroxide which reacts with tissue iron to generate hydroxyl free radicals. Long-term potentiation...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90060-2
更新日期：1991-01-01 00:00:00
abstract：:While systemic capsaicin in adult rats is known to reduce substance P and somatostatin in primary sensory nerves, it is still unknown if it also affects the production of these peptides at the genetic level. Therefore, we examined the effects of systemically administered capsaicin on the expression of the beta-preprot...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00334-x
更新日期：1997-01-01 00:00:00
abstract：:The concentrations of fourteen neurochemicals associated with metabolism, neurotransmission, antioxidant capacity, and cellular structure were measured noninvasively from two distinct brain regions using 1H magnetic resonance spectroscopy. Seventeen young adults (age 19-22years) and sixteen cognitively normal older ad...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.04.035
更新日期：2017-06-23 00:00:00
abstract：:Our study examined how different housing conditions modulated the acquisition of a spatial reference memory task and also, a reversal task in the 4-radial arm water maze (4-RAWM). The animals were randomly assigned to standard or enriched cages, and, as a type of complementary stimulation along with the environmental ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.08.038
更新日期：2015-10-29 00:00:00
abstract：:The dorsal portion of the nucleus submedius of the rat thalamus receives spinal and trigeminal projections which may convey noxious inputs. The present study was undertaken to analyse the fine structure of the nucleus with particular reference to a possible trigemino-thalamo-prefrontal cortical pathway relaying in nuc...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(88)90133-9
更新日期：1988-07-01 00:00:00
abstract：:Aging occurs due to a combination of several factors, such as telomere attrition, cellular senescence, and stem cell exhaustion. The telomere attrition-dependent cellular senescence is regulated by increased levels of SMAD specific E3 ubiquitin protein ligase 2 (smurf2). With age smurf2 expression increases and Smurf2...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.04.003
更新日期：2020-06-01 00:00:00
abstract：:Parkinson's disease (PD) is a common movement disorder. Alpha-synuclein (α-synuclein) plays a critical role in PD. In this study, we evaluated the level of central nervous system (CNS)-derived exosomal α-synuclein in serum, which may be regarded as a specific peripheral biomarker for PD. We recruited patients with PD ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.05.015
更新日期：2019-08-10 00:00:00
abstract：:Rats exposed to repeated unavoidable stress show decreased dopamine output in the nucleus accumbens shell (NAcS) and do not acquire vanilla sugar (VS)-sustained appetitive behavior (VAB). Rats treated with lithium for 3 weeks also show decreased NAcS dopamine output, yet they acquire VAB. Feeding a novel palatable foo...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(03)00383-x
更新日期：2003-01-01 00:00:00
abstract：:We have recently shown that the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate enhances the depolarization induced, calcium dependent release of [3H]dopamine from cultured brain neurons in the rat. In the present study the effects of 12-O-tetradecanoyl-phorbol-13-acetate on the kinetic parameters of depolarizatio...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(86)90140-5
更新日期：1986-12-01 00:00:00
abstract：:Whole-cell voltage-clamp recordings were made from acutely dissociated neurons obtained from the embryonic chick ciliary ganglion. Recording pipettes were filled with salines containing 120 mM CsCl or 120 mM tetraethylammonium-Cl. Application of depolarizing voltage commands evoked L-type Ca2+ currents and, at voltage...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90086-4
更新日期：1991-01-01 00:00:00
abstract：:Olvanil ((N-vanillyl)-9-oleamide), a non-pungent transient receptor potential vanilloid type 1 agonist, desensitizes nociceptors and alleviates pain. But its molecular targets and signaling mechanisms are little known. Calcium influx through voltage-activated Ca(2+) channels plays an important role in neurotransmitter...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.03.023
更新日期：2006-08-11 00:00:00
abstract：:The innervation of the suprachiasmatic nucleus by serotoninergic fibers has been studied in rats from the 22nd embryonic until the 21st postnatal day. The serotoninergic fibers were detected with the pre-embedding immunocytochemical technique at the electron-microscopic level using antibodies to serotonin. Serotonin-i...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90163-5
更新日期：1994-01-01 00:00:00
abstract：:A lack of thyroid hormone in the postnatal period causes an irreversible mental retardation, characterized by a slowing of thoughts and movements accompanied by prolonged latencies of several evoked potentials and slowed electroencephalographic rhythms. Here we show that in cultured hippocampal and cortical neurons fr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.01.047
更新日期：2004-01-01 00:00:00
abstract：:The responses of non-dopamine neurons in substantia nigra and ventral tegmental area to systemic amphetamine were investigated in the behaving rat chronically implanted with multiple fine-wire electrodes. The neurons were identified with electrophysiological criteria requiring that the signals be of biphasic shape, sh...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(88)90343-0
更新日期：1988-02-01 00:00:00
abstract：:Nerve cells have been found in hydra, which react with antisera to oxytocin, vasopressin and mesotocin. These nerve cells have a high density in the ectoderm of basal disk and tentacles and lower density in the ectoderm of peduncle, gastric region and hypostome. A very small number of nerve cells occur also in the end...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(82)90241-x
更新日期：1982-01-01 00:00:00
abstract：:In amyotrophic lateral sclerosis (ALS) there is a selective degeneration of motor neurons leading to muscle paralysis and death. The mechanism underlying cell demise in ALS is not fully understood, but involves the activation of different proteolytic enzymes, including the caspase family of cysteine proteases. We have...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.07.048
更新日期：2006-12-01 00:00:00
abstract：:The interaction between the dopaminergic and glutamatergic systems governs normal behavior and is perturbed in many psychiatric disorders including schizophrenia. Hypofunction of the D1 family of receptors, to which the D(1) and D(5) subtypes belong, is a typical feature of schizophrenia. Here we have used confocal li...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.08.052
更新日期：2009-12-01 00:00:00
abstract：:Using spinal cord and dorsal root ganglion cell cultures, we have studied the immuno-histochemical distribution of several peptide products of the dynorphin gene. With antibody directed toward the midregion of dynorphin A, peptide-immunoreactivity was found exclusively in the cell bodies of spinal cord neurons. Antibo...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(86)90110-7
更新日期：1986-08-01 00:00:00
abstract：:Physiological (pH 7.2 and 7.0) and pathological (pH 6.8 and 6.6) changes of external pH, as they are measured in vivo, were imposed on mouse astrocytes in primary cultures and the effect on components of K+ transport pathways across the cell membrane was measured. Physiological pH changes had no effect at all. Patholo...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(87)90024-8
更新日期：1987-01-01 00:00:00
abstract：:Spreading depolarizations are accompanied by transient changes in cerebral blood flow (CBF). In a post hoc analysis of previously studied control rats we analyzed CBF time courses after middle cerebral artery occlusion in the rat in order to test whether intra-ischemic flow, reperfusion, and different parameters of pe...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.08.049
更新日期：2012-12-13 00:00:00
abstract：:An electropharmacological analysis of the type(s) of calcium channel controlling neurotransmitter release in preganglionic sympathetic nerve terminals in the guinea-pig anterior pelvic ganglion has been carried out. Conventional intracellular recording techniques were used to record excitatory postsynaptic potentials ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00286-9
更新日期：1999-01-01 00:00:00
abstract：:This immunocytochemical study, using a double-staining method, showed that calcitonin gene-related peptide-like immunoreactive structures are widely distributed in the peripheral nervous system and that many of them coexist with substance P-like immunoreactive structures in single sensory ganglion cells. Neurons posit...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(85)90265-9
更新日期：1985-08-01 00:00:00
abstract：:Astrocytes in the somatosensory ventrobasal (VB) thalamus of rats respond to glutamatergic synaptic input with metabotropic glutamate receptor (mGluR) mediated intracellular calcium ([Ca²⁺](i)) elevations. Astrocytes in the VB thalamus also release the gliotransmitter (GT) glutamate in a Ca²⁺-dependent manner. The tri...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.12.049
更新日期：2012-03-15 00:00:00
abstract：:I.v. injection of secretin activates neurons in brain areas controlling autonomic function and emotion. Peripheral administration of secretin inhibits gastric functions through a central mechanism that is mediated by vagal dependent pathways. We investigated whether the vagus nerve is involved in i.p. injection of sec...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.06.027
更新日期：2004-01-01 00:00:00
abstract：:Oxytocin and vasopressin binding sites were localized and characterized by quantitative autoradiography on consecutive sections of Long-Evans rat forebrains and pituitary glands, incubated in the presence of 5 nM [3H]oxytocin or 5 nM [3H]vasopressin. In the forebrain, two types of neurohypophysial hormone binding site...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(88)90143-1
更新日期：1988-07-01 00:00:00
abstract：:Oxidative stress is central to the pathology of several neurodegenerative diseases, including Parkinson's disease (PD), and therapeutics designed to enhance antioxidant potential could have clinical value. In this study, we investigated whether dimethyl fumarate (DMF) has therapeutic effects in cellular and animal mod...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2014.11.047
更新日期：2015-02-12 00:00:00
abstract：:The presynaptic changes caused by 4-aminopyridine were studied in the electric organ of Torpedo marmorata, in the resting state and during the period following transmission of a single giant discharge. Incubation with 4-aminopyridine provoked a 30-40% decrease in the density of synaptic vesicles in nerve terminals, an...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(87)90367-8
更新日期：1987-08-01 00:00:00
abstract：:In the nervous system, the aminoacylhistidine dipeptide carnosine (beta-alanyl-L-histidine) has been shown to be expressed in the olfactory receptor neurons and in brain astrocytes. Using immunocytochemical techniques, we report here a dense carnosine-like immunoreactivity in the subependymal layer of the rodent foreb...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00596-4
更新日期：1998-07-01 00:00:00
abstract：:Simvastatin is an HMG-CoA reductase inhibitor commonly used in the clinic to treat hypercholesterolemia. In addition, simvastatin has been shown to cross the blood-brain barrier and pleiotropic effects of simvastatin have been reported including anti-inflammatory properties, enhancement of neurite outgrowth, and memor...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.02.023
更新日期：2015-04-16 00:00:00
