KRAS mutation testing in clinical practice.


:Activating mutation of KRAS plays a significant role in the pathogenesis of common human malignancies and molecular testing of KRAS mutation has emerged as an essential biomarker in the current practice of clinical oncology. The presence of KRAS mutation is generally associated with clinical aggressiveness of the cancer and reduced survival of the patient. Therapeutically, KRAS mutation testing has maximum utility in stratifying metastatic colorectal carcinoma and lung cancer patients for treatment with targeted therapy. Diagnostically, KRAS mutation testing is useful in the workup of pancreaticobiliary and thyroid cancers, particularly using cytological specimens. In the era of precision medicine, the role of KRAS mutation testing is poised to expand, likely in a setting of combinatorial therapeutic strategy and requiring additional mutation testing of its upstream and/or downstream effectors.


Expert Rev Mol Diagn


Perincheri S,Hui P




Has Abstract


2015-03-01 00:00:00












  • Overview of procalcitonin assays and procalcitonin-guided protocols for the management of patients with infections and sepsis.

    abstract:INTRODUCTION:Procalcitonin is a surrogate infection blood marker whose levels help estimate the likelihood of bacterial infections and correlate with their resolution. Recent trials have revealed the benefits of inclusion of procalcitonin in antibiotic stewardship protocols for initiation and discontinuation of antimic...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Schuetz P,Bretscher C,Bernasconi L,Mueller B

    更新日期:2017-06-01 00:00:00

  • PD-loop technology: PNA openers at work.

    abstract::A concise survey of the emerging PD-loop technology is presented, which outlines several exemplary methods with robust DNA diagnostic potential: duplex DNA capture, topological DNA labeling, nondenaturing DNA sequencing and hybridization of molecular beacons to double-stranded DNA. Advantages of these new PNA-based as...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章


    authors: Demidov VV

    更新日期:2001-09-01 00:00:00

  • The Presage(®) ST2 Assay: analytical considerations and clinical applications for a high-sensitivity assay for measurement of soluble ST2.

    abstract::The Presage(®) ST2 Assay (Critical Diagnostics, CA, USA) is an in vitro diagnostic device that quantitatively measures soluble suppression of tumorigenicity 2 (sST2) in serum and plasma by ELISA. This assay is US FDA approved and is indicated to be used in conjunction with clinical evaluation as an aid in assessing th...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Mueller T,Dieplinger B

    更新日期:2013-01-01 00:00:00

  • Genetic variation in immune function and susceptibility to human filariasis.

    abstract::The generation of a draft sequence of a the human genome has provided the opportunity to characterize human diversity, even as it pertains to differences in host response to parasitic infection with organisms that cause lymphatic filariasis, malaria and schistosomiasis. Worldwide, human infection with filarial pathoge...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Choi EH,Nutman TB,Chanock SJ

    更新日期:2003-05-01 00:00:00

  • TRAC in high-content gene expression analysis: applications in microbial population studies, process biotechnology and biomedical research.

    abstract::More than a decade of intensive use of microarray technology has flooded the scientific community with genome-wide expression data of diverse biological states. As a result, connection of the expression signatures of a relatively small number of genes related to, for example, disease states, patient responses or toxic...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Rautio JJ,Satokari R,Vehmaan-Kreula P,Serkkola E,Söderlund H

    更新日期:2008-07-01 00:00:00

  • Current concepts in the genetic diagnostics of rheumatoid arthritis.

    abstract::Rheumatoid arthritis (RA) is a systemic, chronic and inflammatory disease of unknown etiology. HLA-DRB1 and PTPN22 1858T gene variants are risk factors of RA, clinical manifestations and rate of progression of joint destruction in this autoimmune disease. Currently, several immunopathogenetic models of other genes (CT...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章


    authors: Nagy ZB,Csanád M,Tóth K,Börzsönyi B,Demendi C,Rigó J Jr,Joó JG

    更新日期:2010-07-01 00:00:00

  • Klinefelter syndrome: clinical and molecular aspects.

    abstract::Klinefelter syndrome is the most common chromosome abnormality in humans. The estimated prevalence is one in 500 to one in 1000 males but due to the widely variable and often aspecific features, only one in four cases are recognized. The most specific clinical features which can be observed at adult age are small test...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Giltay JC,Maiburg MC

    更新日期:2010-09-01 00:00:00

  • Genomic tests to guide prostate cancer management following diagnosis.

    abstract:INTRODUCTION:Prostate cancer (PCa) is a common cancer in men, but variable clinical behaviors make its management challenging. Risk stratification is a key issue in disease management. Patient-tailored strategies are strongly advocated to reduce unnecessary treatment while maximizing the oncological outcomes of patient...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Colicchia M,Morlacco A,Cheville JC,Karnes RJ

    更新日期:2017-04-01 00:00:00

  • Management of metastatic renal cell carcinoma: current trends.

    abstract::Renal cell carcinoma is one of the common malignancies of the genitourinary tract. In approximately one third of patients, distant metastases are present at the time of initial diagnosis and in another third, the tumor will recur even after nephrectomy with a curative intent. Renal cell carcinoma is resistant to all c...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Mohammed A,Shergill I,Little B

    更新日期:2009-01-01 00:00:00

  • Application of fetal DNA in maternal plasma for noninvasive prenatal diagnosis.

    abstract::Prenatal diagnosis of fetal genetic conditions is a standard part of modern obstetric care. Many of the current methods rely on invasive methods and are associated with an inherent risk of fetal loss. Consequently, there has been a long-term goal for development of noninvasive prenatal diagnostic methods. In 1997, the...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Chiu RW,Lo YM

    更新日期:2002-01-01 00:00:00

  • Rapid tests for detection of viral markers in blood transfusion.

    abstract::Since the early 1990s, rapid tests have been available for detection of HIV infection. They were intended for field diagnosis, emergency and home testing. In addition, rapid tests for anti-HIV, hepatitis B surface antigen and antihepatitis C virus have been used for blood screening in many resource-poor areas to save ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章


    authors: Allain JP,Lee H

    更新日期:2005-01-01 00:00:00

  • Contemporary genetics-based diagnostics of male infertility.

    abstract::Introduction Thousands of genes are implicated in spermatogenesis, testicular development and endocrine regulation of testicular function. The genetic contribution to male infertility is therefore considerable, and basic and clinical research in the last years found a number of genes that could potentially be used in ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Ferlin A,Dipresa S,Delbarba A,Maffezzoni F,Porcelli T,Cappelli C,Foresta C

    更新日期:2019-07-01 00:00:00

  • High serum caspase-3 levels in hepatocellular carcinoma prior to liver transplantation and high mortality risk during the first year after liver transplantation.

    abstract::Background: Higher liver caspase-3 activity has been found in patients with different liver diseases. However, there is no published data about circulating caspase-3 levels in patients with hepatocellular carcinoma (HCC) who underwent liver transplantation (LT). Therefore, our objective in this study was to determine ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章


    authors: Lorente L,Rodriguez ST,Sanz P,González-Rivero AF,Pérez-Cejas A,Padilla J,Díaz D,González A,Martín MM,Jiménez A,Cerro P,Portero J,Barrera MA

    更新日期:2019-07-01 00:00:00

  • Hereditary sensorineural hearing loss: advances in molecular genetics and mutation analysis.

    abstract::Hearing loss has a genetic etiology in the majority of cases and is very common. The universal newborn hearing screening program, together with remarkable recent progress in the characterization of genes associated with the function of hearing, have resulted in increased demand and exciting possibilities of detecting ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Schrijver I,Gardner P

    更新日期:2006-05-01 00:00:00

  • Prognostic and predictive biomarkers in prostate cancer.

    abstract::Prostate cancer (PCa) is one of the leading causes of cancer death among males, especially in more developed countries. Diagnosis is often achieved at an early stage of the disease with prostate biopsy, following a screening test showing elevated serum levels of prostate-specific antigen or a positive digital rectal e...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Kontos CK,Adamopoulos PG,Scorilas A

    更新日期:2015-01-01 00:00:00

  • Lipoprotein-associated phospholipase A2: a new biomarker for cardiovascular risk assessment and potential therapeutic target.

    abstract::Lipoprotein-associated phospholipase (Lp-PL)A2 is a recently described and potentially useful plasma biomarker associated with cardiovascular disease. The enzyme, originally named platelet-activating factor acetylhydrolase (PAF-AH), has two prominent biological activities. First, it inactivates the prominent proinflam...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Carlquist JF,Muhlestein JB,Anderson JL

    更新日期:2007-09-01 00:00:00

  • Contribution of serum biomarkers to the diagnosis of invasive candidiasis.

    abstract::Invasive candidiasis is the most important opportunistic fungal infection, causing high morbidity and mortality. Traditional methods of diagnosis, which include blood culture and biopsy, usually lack both sensitivity and specificity, or become positive late in the course of the infection. Therefore, new nonculture-bas...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Laín A,Elguezabal N,Moragues MD,García-Ruiz JC,del Palacio A,Pontón J

    更新日期:2008-05-01 00:00:00

  • Next-generation sequencing in precision oncology: challenges and opportunities.

    abstract::High throughput gene sequencing is transforming the utilization of genomics in patient care by providing physicians with a powerful tool to aid the diagnosis and management of disease, particularly in precision oncology. As next-generation sequencing (NGS)-based diagnostic assays are developed, significant hurdles suc...

    journal_title:Expert review of molecular diagnostics

    pub_type: 社论


    authors: Kruglyak KM,Lin E,Ong FS

    更新日期:2014-07-01 00:00:00

  • Molecular analysis of the destruction of articular joint tissues by Raman spectroscopy.

    abstract:INTRODUCTION:Osteoarthritis (OA) is a highly heterogenous disease influenced by different molecular, anatomic, and physiologic imbalances. Some of the bottlenecks for enhanced diagnosis and therapeutic assessment are the lack of validated biomarkers and early diagnosis tools. In this narrative review, we analyze the po...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章


    authors: Casal-Beiroa P,González P,Blanco FJ,Magalhães J

    更新日期:2020-08-01 00:00:00

  • Primary and secondary prevention of cervical cancer.

    abstract::The burden of cervical cancer is very high in low-resource countries, while it is lower in several high-income countries. Secondary prevention based on cervical screening has been very successful in countries where the resources exist to ensure high-quality and good coverage of the population at risk. In many develope...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Grce M

    更新日期:2009-11-01 00:00:00

  • Immune-related biomarkers for diagnosis/prognosis and therapy monitoring of cutaneous melanoma.

    abstract::Skin melanoma, a life-threatening disease, has a recently reported worldwide increase in incidence, despite primary prevention. Skin melanoma statistics emphasize the need for finding markers related to the immune response of the host. The mechanisms that are able to over-power the local immune surveillance comprise m...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Neagu M,Constantin C,Tanase C

    更新日期:2010-10-01 00:00:00

  • Rapid aneuploidy screening (FISH or QF-PCR): the changing scene in prenatal diagnosis?

    abstract::The accuracy of new molecular diagnostics, fluoresence in situ hybridization or quantitative fluorescence-PCR (collectively known as rapid aneuploidy screening), in prenatal diagnosis has already been demonstrated in a number of large studies. The challenge now is how to apply them clinically in the most cost-effectiv...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Leung WC,Lau ET,Lao TT,Tang MH

    更新日期:2004-05-01 00:00:00

  • Fluorescence in situ hybridization: a multitarget approach in diagnosis and management of urothelial cancer.

    abstract::Noninvasive tests for detecting genetic or molecular alterations in urine indicative of urothelial cancer are increasingly becoming the focus of urological cancer research. Since its approval by the US FDA in 2001, the fluorescence in situ hybridization test (Vysis UroVysion) has been widely evaluated. In general, pub...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Arentsen HC,de la Rosette JJ,de Reijke TM,Langbein S

    更新日期:2007-01-01 00:00:00

  • A review of novel technologies and techniques associated with identification of bloodstream infection etiologies and rapid antimicrobial genotypic and quantitative phenotypic determination.

    abstract:INTRODUCTION:The antimicrobial aspect of management of patients with blood stream infections (BSI) and sepsis is time critical. In an era of increasing antimicrobial resistance, rapid detection and identification of bacteria with antimicrobial susceptibility is crucial to direct therapy early in the course of illness. ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Poole S,Kidd SP,Saeed K

    更新日期:2018-06-01 00:00:00

  • Information management driven by diagnostic patient data: right information for the right patient.

    abstract::Existing and future diagnostic technologies are providing a huge amount of data about the patient, which are to be sensibly managed by the treating physician. More than that, the sheer complexity of the interrelation between these data are calling for a radically new approach in the handling of medical data and inform...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Fierz W

    更新日期:2002-07-01 00:00:00

  • CSF biomarkers for Alzheimer's disease: use in early diagnosis and evaluation of drug treatment.

    abstract::Early diagnosis of Alzheimer's disease is important in initiating symptomatic treatment with acetylcholine esterase inhibitors, and will be of even greater significance if drugs with a potential to slow down the degenerative process, such as beta-secretase inhibitors and beta-amyloid vaccination, prove to have a clini...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Blennow K

    更新日期:2005-09-01 00:00:00

  • Application of mass spectrometry to molecular diagnostics of viral infections.

    abstract::Mass spectrometry (MS) has found numerous applications in life sciences. It has high accuracy, sensitivity and wide dynamic range in addition to medium- to high-throughput capabilities. These features make MS a superior platform for analysis of various biomolecules including proteins, lipids, nucleic acids and carbohy...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Ganova-Raeva LM,Khudyakov YE

    更新日期:2013-05-01 00:00:00

  • Emergent FDA biodefense issues for microarray technology: process analytical technology.

    abstract::A successful biodefense strategy relies upon any combination of four approaches. A nation can protect its troops and citizenry first by advanced mass vaccination, second, by responsive ring vaccination, and third, by post-exposure therapeutic treatment (including vaccine therapies). Finally, protection can be achieved...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章


    authors: Weinberg S

    更新日期:2004-11-01 00:00:00

  • Plasma methylated septin 9: a colorectal cancer screening marker.

    abstract::Colorectal cancer (CRC) is a slow-developing cancer (10-15 years) with one of the highest frequencies in the world's population. Many countries have implemented various CRC screening programs, but have not achieved the desired compliance. Colonoscopy - considered the gold standard for CRC screening - has its limitatio...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章


    authors: Molnár B,Tóth K,Barták BK,Tulassay Z

    更新日期:2015-02-01 00:00:00

  • Evaluating the use of PCR for diagnosing invasive aspergillosis.

    abstract:INTRODUCTION:Aspergillus species, primarily Aspergillus fumigatus, are still the most emerging fungal pathogens. Within recent years, novel molecular methods have been developed to improve the diagnosis of life-threatening invasive aspergillosis in high risk patients. Especially patients with malignant hematological di...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审


    authors: Buchheidt D,Reinwald M,Hofmann WK,Boch T,Spiess B

    更新日期:2017-06-01 00:00:00