Lung cancer screening: utility of molecular applications in conjunction with low-dose computed tomography guidelines.

abstract：:Since aptamers were reported in 1990, research into the applications of aptamers, particularly diagnostic applications, has been growing. Aptamers can act as recognition elements instead of antibodies. In this regard, aptamers have unique characteristics because they are composed of nucleic acids. Intra- and intermole...

journal_title：Expert review of molecular diagnostics

authors： Yoshida W,Abe K,Ikebukuro K

更新日期：2014-03-01 00:00:00

abstract：:Mass spectrometry (MS) has found numerous applications in life sciences. It has high accuracy, sensitivity and wide dynamic range in addition to medium- to high-throughput capabilities. These features make MS a superior platform for analysis of various biomolecules including proteins, lipids, nucleic acids and carbohy...

journal_title：Expert review of molecular diagnostics

authors： Ganova-Raeva LM,Khudyakov YE

更新日期：2013-05-01 00:00:00

abstract：:High throughput gene sequencing is transforming the utilization of genomics in patient care by providing physicians with a powerful tool to aid the diagnosis and management of disease, particularly in precision oncology. As next-generation sequencing (NGS)-based diagnostic assays are developed, significant hurdles suc...

journal_title：Expert review of molecular diagnostics

authors： Kruglyak KM,Lin E,Ong FS

更新日期：2014-07-01 00:00:00

abstract：:A concise survey of the emerging PD-loop technology is presented, which outlines several exemplary methods with robust DNA diagnostic potential: duplex DNA capture, topological DNA labeling, nondenaturing DNA sequencing and hybridization of molecular beacons to double-stranded DNA. Advantages of these new PNA-based as...

journal_title：Expert review of molecular diagnostics

authors： Demidov VV

更新日期：2001-09-01 00:00:00

abstract：:Viral-associated malignancies usually arise in the setting of altered immunity or with declines in immune function associated with aging. The main culprits are the lymphotropic herpesvirus, including Epstein-Barr virus (EBV) and human herpesvirus-8, which are the focus of this review. Chronic persistent infection and ...

journal_title：Expert review of molecular diagnostics

authors： Yin CC,Jones D

更新日期：2006-11-01 00:00:00

abstract：:Acute intermittent porphyria (AIP) is an inherited metabolic disease with an autosomal dominant pattern of inheritance. The disease is caused by a partial deficiency of porphobilinogen deaminase (PBGD) in heme biosynthesis. Since biochemical measurements of patients and their healthy relatives overlap, the diagnosis o...

journal_title：Expert review of molecular diagnostics

authors： Kauppinen R

更新日期：2004-03-01 00:00:00

abstract：:The emergence of drug resistance remains one of the most challenging issues in the treatment of HIV-1 infection. The extreme replication dynamics of HIV facilitates its escape from the selective pressure exerted by the human immune system and by the applied combination drug therapy. This article reviews computational ...

journal_title：Expert review of molecular diagnostics

authors： Cordes F,Kaiser R,Selbig J

更新日期：2006-03-01 00:00:00

abstract：AIM:Molecular diagnosis of fragile X syndrome demands assessment of fragile X mental retardation 1 (FMR1) CGG repeat size and methylation status, while predicting disease transmission risk requires determination of AGG interruption pattern. There is currently no single assay that provides all three categories of inform...

journal_title：Expert review of molecular diagnostics

authors： Rajan-Babu IS,Teo CR,Lian M,Lee CG,Law HY,Chong SS

更新日期：2015-03-01 00:00:00

abstract：:Concomitant advances made by the Human Genome Project and in the development of nucleic acid screening technologies are driving the expansion of pharmacogenomic research and molecular diagnostics. However, most current technologies are restrictive due to their complexity and/or cost, limiting the potential of personal...

journal_title：Expert review of molecular diagnostics

authors： de Arruda M,Lyamichev VI,Eis PS,Iszczyszyn W,Kwiatkowski RW,Law SM,Olson MC,Rasmussen EB

更新日期：2002-09-01 00:00:00

abstract：INTRODUCTION:Rapidity, simplicity, and portability are highly desirable characteristics of tests and devices designed for performing diagnostics at the point of care (POC), either near patients managed in healthcare facilities or to offer bioanalytical alternatives in external settings. By reducing the turnaround time ...

journal_title：Expert review of molecular diagnostics

authors： Bissonnette L,Bergeron MG

更新日期：2017-05-01 00:00:00

abstract：:Genetic mapping by linkage analysis has been an invaluable tool in the positional strategy to identify the molecular basis of many rare Mendelian disorders. With the attention of the scientific and medical community shifting towards the analysis of more common, complex traits, it has become necessary to develop new ap...

journal_title：Expert review of molecular diagnostics

authors： Forabosco P,Falchi M,Devoto M

更新日期：2005-09-01 00:00:00

abstract：:Cancer becomes the second leading cause of death in the world. An effective strategy for early diagnosis of the disease is key to reduce the mortality and morbidity. Development of effective monoclonal antibody (mAb)-based assays or diagnostic imaging techniques for detection of antigens and small molecules that are r...

journal_title：Expert review of molecular diagnostics

authors： Zhang X,Soori G,Dobleman TJ,Xiao GG

更新日期：2014-01-01 00:00:00

abstract：:Activating mutation of KRAS plays a significant role in the pathogenesis of common human malignancies and molecular testing of KRAS mutation has emerged as an essential biomarker in the current practice of clinical oncology. The presence of KRAS mutation is generally associated with clinical aggressiveness of the canc...

journal_title：Expert review of molecular diagnostics

authors： Perincheri S,Hui P

更新日期：2015-03-01 00:00:00

abstract：:The accuracy of new molecular diagnostics, fluoresence in situ hybridization or quantitative fluorescence-PCR (collectively known as rapid aneuploidy screening), in prenatal diagnosis has already been demonstrated in a number of large studies. The challenge now is how to apply them clinically in the most cost-effectiv...

journal_title：Expert review of molecular diagnostics

authors： Leung WC,Lau ET,Lao TT,Tang MH

更新日期：2004-05-01 00:00:00

abstract：INTRODUCTION:A classification of rhabdomyosarcoma (RMS) with prognostic relevance has primarily relied on clinical features and histologic classification as either embryonal or alveolar RMS. The PAX3-FOXO1 and PAX7-FOXO1 gene fusions occur in 80% of cases with the alveolar subtype and are more predictive of outcome tha...

journal_title：Expert review of molecular diagnostics

authors： Arnold MA,Barr FG

更新日期：2017-02-01 00:00:00

abstract：:Acute pancreatitis has a mortality rate of 5-10%. Early deaths are mainly due to multiorgan failure and late deaths are due to septic complications from pancreatic necrosis. The recently described 2012 Revised Atlanta Classification and the Determinant Classification both provide a more accurate description of edemato...

journal_title：Expert review of molecular diagnostics

authors： Gomatos IP,Xiaodong X,Ghaneh P,Halloran C,Raraty M,Lane B,Sutton R,Neoptolemos JP

更新日期：2014-04-01 00:00:00

abstract：:The possibility to detect viral DNA or RNA in a quantitative manner has already contributed significantly to the management and diagnosis of viral infections, as well as to the understanding of virus-host interactions. New developments in amplification techniques based on real-time detection, as well as automation of ...

journal_title：Expert review of molecular diagnostics

authors： Schutten M,Niesters HG

更新日期：2001-07-01 00:00:00

abstract：:This review focuses on the fluorescent protein microarrays applied to neurodegenerative disorders, a major health problem in our aging society. Biomarker discovery studies and work on new diagnostic tests are both included. Three platforms are described: antibody planar microarrays, comprising an array of well-defined...

journal_title：Expert review of molecular diagnostics

authors： Cretich M,Sola L,Gagni P,Chiari M

更新日期：2013-11-01 00:00:00

abstract：:The measurement of numerous samples as in drug screening or diagnostics has been improved significantly over recent years. The processing of a great number of carriers with 96, 384 or 1536 wells is not the limiting step anymore and more than 100,000 samples can be analyzed within 24 h. New challenges arise in optimizi...

journal_title：Expert review of molecular diagnostics

authors： Schaertl S,Meyer-Almes FJ

更新日期：2001-11-01 00:00:00

abstract：:More than a decade of intensive use of microarray technology has flooded the scientific community with genome-wide expression data of diverse biological states. As a result, connection of the expression signatures of a relatively small number of genes related to, for example, disease states, patient responses or toxic...

journal_title：Expert review of molecular diagnostics

authors： Rautio JJ,Satokari R,Vehmaan-Kreula P,Serkkola E,Söderlund H

更新日期：2008-07-01 00:00:00

abstract：INTRODUCTION:Drug-induced liver injury (DILI) is a severe adverse drug reaction which is of major concern to patients, clinicians and the pharmaceutical industry. Accurate and rapid detection of DILI is important for patient stratification and treatment in the clinic and benefits preclinical drug design and risk assess...

journal_title：Expert review of molecular diagnostics

authors： Howell LS,Ireland L,Park BK,Goldring CE

更新日期：2018-01-01 00:00:00

abstract：:The therapeutic management of patients with lupus nephritis (LN) remains a major challenge. The availability of biomarkers that accurately predict renal flares, response to immunosuppressive treatment and risk of progression to end-stage renal disease would allow the more effective use of currently available immunosup...

journal_title：Expert review of molecular diagnostics

authors： Edelbauer M,Ho J

更新日期：2011-07-01 00:00:00

abstract：INTRODUCTION:Cytomegalovirus (CMV) is a common opportunistic infection that contributes to poor outcomes in hematopoietic stem cell transplant (HSCT) recipients. Prevention of CMV end-organ disease in allogeneic HSCT recipients is commonly achieved by preemptive antiviral therapy of asymptomatic CMV reactivation that i...

journal_title：Expert review of molecular diagnostics

authors： Ramanan P,Razonable RR

更新日期：2017-07-01 00:00:00

abstract：:The capacity to use molecular techniques to establish the genetic diagnoses of the autosomal dominant ataxias has revolutionized the field. It is now possible to systematically classify these disorders according to the nature of the causative mutation, with implications for diagnostic testing, analysis of pathogenesis...

journal_title：Expert review of molecular diagnostics

authors： Margolis RL

更新日期：2003-11-01 00:00:00

abstract：:The knowledge that the organism's metabolome is a potentially informative mirror of the impact of disease and its dynamics has led to promising developments in cancer research, strongly geared toward the discovery of new biomarkers of disease onset and progression. The present text reviews the advances made in the las...

journal_title：Expert review of molecular diagnostics

authors： Duarte IF,Rocha CM,Gil AM

更新日期：2013-09-01 00:00:00

abstract：:EU regulations stipulate the labeling of food products containing genetically modified organisms (GMOs) unless the GMO content is due to adventitious and unintended 'contamination' and not exceeding the 1% level at ingredient basis. In addition, member states have to ensure full traceability at all stages of the placi...

journal_title：Expert review of molecular diagnostics

authors： Aarts HJ,van Rie JP,Kok EJ

更新日期：2002-01-01 00:00:00

abstract：:Introduction: The use of liquid biopsy on the blood from solid malignancies provides a convenient way of detecting actionable mutations, monitoring treatment response, detecting early recurrence and prognosticating outcomes. The aim of this review is to discuss the current status and future direction of serum biomarke...

journal_title：Expert review of molecular diagnostics

authors： Kouba E,Lopez-Beltran A,Montironi R,Massari F,Huang K,Santoni M,Chovanec M,Cheng M,Scarpelli M,Zhang J,Cimadamore A,Cheng L

更新日期：2020-02-01 00:00:00

abstract：:The generation of a draft sequence of a the human genome has provided the opportunity to characterize human diversity, even as it pertains to differences in host response to parasitic infection with organisms that cause lymphatic filariasis, malaria and schistosomiasis. Worldwide, human infection with filarial pathoge...

journal_title：Expert review of molecular diagnostics

authors： Choi EH,Nutman TB,Chanock SJ

更新日期：2003-05-01 00:00:00

abstract：:The emerging field of metabolomics, in which a large number of small-molecule metabolites from body fluids or tissues are detected quantitatively in a single step, promises immense potential for early diagnosis, therapy monitoring and for understanding the pathogenesis of many diseases. Metabolomics methods are mostly...

journal_title：Expert review of molecular diagnostics

authors： Gowda GA,Zhang S,Gu H,Asiago V,Shanaiah N,Raftery D

更新日期：2008-09-01 00:00:00

abstract：:Whole-exome sequencing (WES) represents a significant breakthrough in the field of human genetics. This technology has largely contributed to the identification of new disease-causing genes and is now entering clinical laboratories. WES represents a powerful tool for diagnosis and could reduce the 'diagnostic odyssey'...

journal_title：Expert review of molecular diagnostics

authors： Tetreault M,Bareke E,Nadaf J,Alirezaie N,Majewski J

更新日期：2015-06-01 00:00:00