Abstract:
:Double umbilical cord blood transplantation is increasingly applied in the treatment of adult patients with high-risk hematological malignancies and has been associated with improved engraftment as compared to that provided by single unit cord blood transplantation. The mechanism of improved engraftment is, however, still incompletely understood as only one unit survives. In this multicenter phase II study we evaluated engraftment, early chimerism, recovery of different cell lineages and transplant outcome in 53 patients who underwent double cord blood transplantation preceded by a reduced intensity conditioning regimen. Primary graft failure occurred in one patient. Engraftment was observed in 92% of patients with a median time to neutrophil recovery of 36 days (range, 15-102). Ultimate single donor chimerism was established in 94% of patients. Unit predominance occurred by day 11 after transplantation and early CD4(+) T-cell chimerism predicted for unit survival. Total nucleated cell viability was also associated with unit survival. With a median follow up of 35 months (range, 10-51), the cumulative incidence of relapse and non-relapse mortality rate at 2 years were 39% and 19%, respectively. Progressionfree survival and overall survival rates at 2 years were 42% (95% confidence interval, 28-56) and 57% (95% confidence interval, 43-70), respectively. Double umbilical cord blood transplantation preceded by a reduced intensity conditioning regimen using cyclophosphamide/fludarabine/4 Gy total body irradiation results in a high engraftment rate with low non-relapse mortality. Moreover, prediction of unit survival by early CD4(+) lymphocyte chimerism might suggest a role for CD4(+) lymphocyte mediated unit-versus-unit alloreactivity. www.trialregister.nl NTR1573.
journal_name
Haematologicajournal_title
Haematologicaauthors
Somers JA,Braakman E,van der Holt B,Petersen EJ,Marijt EW,Huisman C,Sintnicolaas K,Oudshoorn M,Groenendijk-Sijnke ME,Brand A,Cornelissen JJdoi
10.3324/haematol.2014.106690subject
Has Abstractpub_date
2014-11-01 00:00:00pages
1753-61issue
11eissn
0390-6078issn
1592-8721pii
haematol.2014.106690journal_volume
99pub_type
杂志文章相关文献
HAEMATOLOGICA文献大全abstract:BACKGROUND AND OBJECTIVES:It is often difficult to obtain good karyotypes of cells from children with acute lymphoblastic leukemia (ALL) because of poor morphology and spreading. Detailed karyotyping can be further hampered by the presence of multiple rearrangements. Our objective was to search for cryptic rearrangemen...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract::The majority of responses produced in patients with low-grade lymphomas are unique among non-Hodgkin's lymphomas (NHL), and even with a more intensive chemotherapy regimen, they are only partial; the very few complete responses which are induced are usually of short duration and do not influence overall survival. Ther...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
abstract::A patient with M5b acute nonlymphoblastic leukemia (ANLL) and a 47,XXX del(11) (q23) karyotype is described. Partial remission was obtained after treatment with daunorubicin, arabinosylcytosine and VP-16. Subsequently, two courses of chemotherapy for resistant ANLL were administered without achieving complete remissio...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND:An effective second-line treatment for intermediate and high grade non-Hodgkin's lymphoma is greatly needed since 30% of patients do not achieved complete remission (CR) and another 20% to 30% of the CRs will eventually relapse. METHODS:A four-drug combination with Mitoxantrone, Etoposide, Cisplatin and Dex...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Whether degree of iron stores influences progression of human immunodeficiency virus (HIV) disease is controversial. We studied the relationship of indirect measures of iron stores with mortality in highly active antiretroviral therapy (HAART)-naive participants from the Women's Interagency HI...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究
doi:
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:Resistance to imatinib is an important clinical issue in the treatment of Philadelphia chromosome-positive leukemias which is being tackled by the development of new, more potent drugs, such as the dual Src/Abl tyrosine kinase inhibitors dasatinib and bosutinib and the imatinib analog nilotinib. In the curre...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.12212
更新日期:2008-05-01 00:00:00
abstract:BACKGROUND:alpha thalassemias are very common in all thalassemic areas; however, complete knowledge of the phenotypic, genotypic and epidemiological features of these thalassemias has not yet been achieved for a number of reasons: the frequent absence of a thalassemic hematologic picture, the lack of a specific charact...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::Differentiation of erythroblasts to mature red blood cells involves dynamic changes of the membrane and cytoskeleton networks that are not fully characterized. Using a mouse fetal liver erythroblast culture system and a targeted shRNA functional screening strategy, we identified a critical role of pleckstrin-2 in acti...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2014.105809
更新日期:2014-07-01 00:00:00
abstract::In childhood B-cell precursor acute lymphoblastic leukemia, cytogenetics is important in diagnosis and as an indicator of response to therapy, thus playing a key role in risk stratification of patients for treatment. Little is known of the relationship between different cytogenetic subtypes in B-cell precursor acute l...
journal_title:Haematologica
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3324/haematol.2013.085175
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Cord blood (CB) is a valuable source of stem cells. Most CB units are still cryopreserved in single bags in the world's CB banks. Thawing a single CB unit, dividing it into two parts, expanding the smaller one and refreezing the other would optimize ex vivo expansion of CB progenitors prior to...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Germline RUNX1 mutations lead to thrombocytopenia and platelet dysfunction in familial platelet disorder with predisposition to acute myelogenous leukemia (AML). Multiple aspects of platelet function are impaired in these patients, associated with altered expression of genes regulated by RUNX1 We aimed to identify RUN...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2018.188904
更新日期:2019-06-01 00:00:00
abstract::Rituximab has improved response rates and overall survival in B-cell lymphoma (DLBCL). Radiotherapy is an effective treatment modality for lymphomas, but there is uncertainty on its use as consolidation after chemo-immunotherapy mainly in advanced stages. We evaluated its efficacy with a comprehensive meta-analysis an...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2020.249680
更新日期:2020-06-18 00:00:00
abstract:BACKGROUND:Dyskeratosis congenita is a cancer-prone bone marrow failure syndrome caused by aberrations in telomere biology. DESIGN AND METHODS:We studied 65 patients with dyskeratosis congenita and 127 unaffected relatives. Telomere length was measured by automated multicolor flow fluorescence in situ hybridization in...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:10.3324/haematol.2011.055269
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Transferrin receptor (TfR) expression in erythroid cells is regulated by a number of factors, including iron status and erythropoietin (Epo) stimulation. However, the impact of these factors on reticulocyte TfR expression in vivo has never been studied. A soluble form of TfR (sTfR) is present ...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract:BACKGROUND:There is variability in the outcome of patients with chronic lymphocytic leukemia with apparently the same stage of disease. Identifying genetic variants that influence patients' outcome and response to treatment may provide important insights into the biology of the disease. DESIGN AND METHODS:We investiga...
journal_title:Haematologica
pub_type: 杂志文章,随机对照试验
doi:10.3324/haematol.2011.043471
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The diagnosis of T-cell neoplasia requires the use of immunohistochemistry on tumor sections or molecular genetic analysis of T-cell receptor (TCR) clonality. Multiplex polymerase chain reactions (PCR) offer a sensitive and expeditious approach to determining clonality early in the diagnostic ...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
abstract::Anemia is highly prevalent, especially in older individuals. In selected populations, anemia has been reported to be associated with impaired survival and health-related quality of life. However, data on this impact in the general population are rare. Furthermore, discussions on the optimal definition of anemia have n...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2018.195552
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Familial aggregation has been recognized in patients with several lymphoid neoplasms, but the genetic basis for this familial clustering is not known. Germ-line mutations in the ataxia-telangiectasia mutated (ATM) and CHK2 genes have been detected in patients with mantle cell lymphoma (MCL), s...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract::Whole blood donors, especially frequently donating donors, have a risk of iron deficiency and low hemoglobin levels, which may affect their health and eligibility to donate. Lifestyle behaviors, such as dietary iron intake and physical activity, may influence iron stores and thereby hemoglobin levels. We aimed to inve...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2019.229450
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:The myeloproliferative neoplasms, essential thrombocytosis, polycythemia vera and primary myelofibrosis, share the same acquired genetic lesion, but the concept of JAK2 V617F serving as the sole lesion responsible for these neoplasms is under question, and there has been interest in identifying additional mu...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2011.045591
更新日期:2011-10-01 00:00:00
abstract::We reviewed cardiac T2* assessments from 77 thalassemia major patients between the ages of 2.5 and 18 years to study optimal timing of cardiac iron screening by magnetic resonance imaging. No patient under 9.5 years of age showed detectable cardiac iron in contrast to 36% of patients between the ages of 15-18 years ol...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.12513
更新日期:2008-06-01 00:00:00
abstract::Hematopoietic differentiation is driven by transcription factors, which orchestrate a finely tuned transcriptional network. At bipotential branching points lineage decisions are made, where key transcription factors initiate cell type-specific gene expression programs. These programs are stabilized by the epigenetic a...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2017.174516
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:The clonal origin of myeloproliferative disorders has been clearly demonstrated and it is known that reactive thrombocytosis occurs as a non specific response to various inflammatory or neoplastic conditions. Only a few papers have discussed the topic of myeloproliferative diseases in blood donors. MATERIAL...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::Leukemia stem cells contribute to drug-resistance and relapse in chronic myeloid leukemia (CML) and BCR-ABL1 inhibitor monotherapy fails to eliminate these cells, thereby necessitating alternate therapeutic strategies for patients CML. The peroxisome proliferator-activated receptor-γ (PPARγ) agonist pioglitazone downr...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2018.194910
更新日期:2020-04-01 00:00:00
abstract::BACKGROUND. Hypofibrinogenemia and increased fibrin(ogen) degradation products in acute leukemia have been attributed to intravascular thrombin generation triggered by leukemic cells. However, the strict relationship between fibrinogen catabolism and turnover of fibrinopeptide A (FPA), which is a sensitive and specifi...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract:BACKGROUND:Polycythemia vera is a clonal hematopoietic stem cell disorder in which the JAK2 V617F mutation is observed in >95% of patients, but an as yet unidentified process appears to initiate the clonal expansion of hematopoiesis. Because microRNA regulate hematopoietic differentiation, we hypothesized that dysregul...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.12706
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:Chronic lymphocytic leukemia has a variable clinical course. Genomic aberrations identify prognostic subgroups, pointing towards distinct underlying biological mechanisms that are poorly understood. In particular it remains unclear whether the prognostic subgroups of chronic lymphocytic leukemia are characte...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.025734
更新日期:2010-11-01 00:00:00
abstract::BCL11A was the focus of recent studies on its inhibiting effect when bound onto the β-globin cluster in the mechanism of hemoglobin switching and HbF downregulation. We examined a cohort of 10 patients displaying different HbF levels and short deletions within the γβ-δ intergenic region to find a possible correlation ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2012.061994
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Pediatric acute myeloid leukemia is a heterogeneous disease characterized by non-random genetic aberrations related to outcome. The genetic subtype is currently detected by different diagnostic procedures which differ in success rate and/or specificity. DESIGN AND METHODS:We examined the potential of gene e...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.029660
更新日期:2011-02-01 00:00:00
abstract::The discovery that the Ten-Eleven Translocation (TET) hydroxylases cause DNA demethylation has fundamentally changed the notion of how DNA methylation is regulated. Clonal analysis of the hematopoetic stem cell compartment suggests that TET2 mutations can be early events in hematologic cancers and recent investigation...
journal_title:Haematologica
pub_type: 杂志文章,随机对照试验
doi:10.3324/haematol.2013.088740
更新日期:2013-12-01 00:00:00