Abstract:
:Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Mascher M,Jost M,Kuon JE,Himmelbach A,Aßfalg A,Beier S,Scholz U,Graner A,Stein Ndoi
10.1186/gb-2014-15-6-r78subject
Has Abstractpub_date
2014-06-10 00:00:00pages
R78issue
6eissn
1474-7596issn
1474-760Xpii
gb-2014-15-6-r78journal_volume
15pub_type
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