Abstract:
:The Killer-cell Immunoglobulin-like Receptor (KIR) gene complex has considerable biomedical importance. Patterns of polymorphism in the KIR region include variability in the gene content of haplotypes and diverse structural arrangements. Droplet digital PCR (ddPCR) was used to identify different haplotype motifs and to enumerate KIR copy number variants (CNVs). ddPCR detected a variety of KIR haplotype configurations in DNA from well-characterized cell lines. Mendelian segregation of ddPCR-estimated KIR2DL5 CNVs was observed in Gambian families and CNV typing of other KIRs was shown to be accurate when compared to an established quantitative PCR method.
journal_name
Genome Medjournal_title
Genome medicineauthors
Roberts CH,Jiang W,Jayaraman J,Trowsdale J,Holland MJ,Traherne JAdoi
10.1186/gm537subject
Has Abstractpub_date
2014-03-05 00:00:00pages
20issue
3issn
1756-994Xpii
gm537journal_volume
6pub_type
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