Thymidine and leucine incorporation in soil bacteria with different cell size.

Abstract:

:Thymidine and leucine incorporation into macromolecules of soil bacteria extracted by homogenization-centrifugation were measured after size-fractionation of the bacterial suspension through different sized filters (1.0, 0.8, 0.6, 0.4 μm). The specific thymidine incorporation rate was highest for the unfiltered and 1.0 μm filtered suspensions (approximately 10 × 10(-21) mol thymidine bacteria(-1) h(-1)), but decreased to 1.39 × 10(-21) mol bacteria(-1) h(-1) for bacteria passing the 0.4 μm filter. The proportion of culturable bacteria (percent colony forming units/acridine orange direct counts) also decreased with bacterial cell size from 5.0% for the unfiltered bacterial suspension to 0.8% in the 0.4 µm filtrate. A strong linear correlation (r (2) = 0.995) was found between the specific thymidine incorporation rate and the proportion of culturable bacteria. Leucine incorporation gave similar results to the thymidine incorporation. No effects of cell size on the degree of isotope dilution or unspecific labeling of other macromolecules were found either for the thymidine or the leucine incorporation technique. These data indicate that small bacteria, although more numerous than larger ones, not only constitute a smaller proportion of the soil bacterial biomass than larger bacteria, but also contribute to a lesser degree to carbon transformations in soil.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Bååth E

doi

10.1007/BF00182410

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

267-78

issue

3

eissn

0095-3628

issn

1432-184X

journal_volume

27

pub_type

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