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Elucidating the native architecture of the YidC: ribosome complex.

Abstract：

:Membrane protein biogenesis in bacteria occurs via dedicated molecular systems SecYEG and YidC that function independently and in cooperation. YidC belongs to the universally conserved Oxa1/Alb3/YidC family of membrane insertases and is believed to associate with translating ribosomes at the membrane surface. Here, we have examined the architecture of the YidC:ribosome complex formed upon YidC-mediated membrane protein insertion. Fluorescence correlation spectroscopy was employed to investigate the complex assembly under physiological conditions. A slightly acidic environment stimulates binding of detergent-solubilized YidC to ribosomes due to electrostatic interactions, while YidC acquires specificity for translating ribosomes at pH-neutral conditions. The nanodisc reconstitution of the YidC to embed it into a native phospholipid membrane environment strongly enhances the YidC:ribosome complex formation. A single copy of YidC suffices for the binding of translating ribosome both in detergent and at the lipid membrane interface, thus being the minimal functional unit. Data reveal molecular details on the insertase functioning and interactions and suggest a new structural model for the YidC:ribosome complex.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kedrov A,Sustarsic M,de Keyzer J,Caumanns JJ,Wu ZC,Driessen AJ

doi

10.1016/j.jmb.2013.07.042

subject

Has Abstract

pub_date

2013-11-15 00:00:00

pages

4112-24

issue

22

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(13)00503-2

journal_volume

425

pub_type

杂志文章

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